Lewis A Blood Group Antigen Antibody (7LE) [Alexa Fluor™ Plus 647]

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 7LE
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 647

Lewis A Blood Group Antigen Antibody (7LE) [Alexa Fluor™ Plus 647] Summary

• Immunogen: Mucin isolated by size-exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification).
• Specificity: The antibody 7LE recognizes Lewisa blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed in colonic epithelial cells. Lewisa may be useful for detection of gastrointestinal, pancreatic and colorectal tumors.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ABO
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Lewis A Blood Group Antigen Antibody (7LE) [Alexa Fluor™ Plus 647]

• A transferase
• A3GALNT
• A3GALT1
• ABO
• B transferase
• EC=2.4.1.37
• EC=2.4.1.40
• Fucosylglycoprotein 3-alpha-galactosyltransferase
• Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form
• Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase
• Glycoprotein-fucosylgalactoside alpha-galactosyltransferase
• Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase
• GTB
• Histo-blood group A transferase
• Histo-blood group ABO system transferase
• Histo-blood group B transferase
• NAGAT

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ABO