Leptin R Antibody (52263) [Unconjugated]

Images

 
Western blot shows 25 ng of Recombinant Human Leptin R Fc Chimera (Catalog # 389-LR) and Recombinant Mouse Leptin R Fc Chimera (Catalog # 497-LR). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human ...read more
Human whole blood monocytes were stained with Human Leptin R Monoclonal Antibody (Catalog # MAB867, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clone
52263
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Leptin R Antibody (52263) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Leptin R
Phe22-Asp839 (predicted)
Accession # P48357
Specificity
Detects human Leptin R in direct ELISAs and Western blots. In Western blots, cross reactivity with recombinant mouse Leptin R is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
LEPR
Purity Statement
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB867 in the following applications:

Publications
Read Publications using
MAB867 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Leptin R Antibody (52263) [Unconjugated]

  • B219
  • CD295 antigen
  • CD295
  • DB
  • DKFZp686B1731
  • huB219
  • LEPR
  • LEP-R
  • Leptin R
  • leptin receptor
  • LeptinR
  • OB R
  • OB receptor
  • OB-R
  • OBRCD295

Background

Leptin receptor (OB-R), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OB-R, including a long form (OB-RL) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OB-Rs) lacking signal-transducing capabilities, have been identified. The extracellular domains of the short and long forms of OB-R are identical. An OB-R transcript, lacking a transmembrane domain and potentially encoding a soluble form of the receptor, has also been described. OB-RL transcripts were reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. Expression of OB-Rs transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney, and primitive hematopoietic cell populations. OB-R has been shown to be encoded by the mouse diabetes (db) and rat fatty (fa) genes. Rodents homozygous for the db or fa mutations have been known to exhibit an obesity phenotype. Human OB-R long form encodes a 1165 amino acid (aa) precursor protein with a 22 aa signal peptide, an 819 aa extracellular domain, a 21 aa transmembrane domain and a 303 aa cytoplasmic domain. The extracellular domain of OB-R contain two hemopoietin receptor domains, a fibronectin type III domain and the WSXWS domain. Recombinant soluble OB-R has been shown to bind Leptin with high affinity and is a potent Leptin antagonist.

  1. Tartaglia, L.A. et al. (1995) Cell 83:1263.
  2. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
  3. Tartaglia, L.A. (1997) J. Biol. Chem. 272:6093.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Leptin R Antibody (MAB867)(4)

Review for Leptin R Antibody (MAB867) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB867:
Filter by Applications
Flow
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Flow Cytometry Leptin R MAB867
Enlarge
4
reviewed by:
Craig Dorrell
Flow Human 12/06/2016
View

Summary

ApplicationFlow Cytometry
Sample TestedHuman pancreatic islet cells
SpeciesHuman
LotDAQ071610A

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Leptin R Antibody (MAB867) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB867U
Alexa Fluor 405 FAB867V
Alexa Fluor 488 FAB867G
Alexa Fluor 532 FAB867X
Alexa Fluor 594 FAB867T
Alexa Fluor 647 FAB867R
Alexa Fluor 700 FAB867N
Alexa Fluor 750 FAB867S
Allophycocyanin FAB867A
Biotin FAB867B
DyLight 350 FAB867D
DyLight 405 FAB867E
DyLight 488 FAB867K
DyLight 550 FAB867L
DyLight 594 FAB867M
DyLight 650 FAB867W
DyLight 680 FAB867Y
DyLight 755 FAB867Z
Fluorescein FAB867F
HRP FAB867H
Janelia Fluor 549 FAB867I
Janelia Fluor 646 FAB867J
PerCP FAB867C
Phycoerythrin FAB867P

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Craig Dorrell
12/06/2016
Application: Flow
Species: Human

Bioinformatics

Gene Symbol LEPR
Uniprot