Leptin R Antibody (52263) [Unconjugated]

Western blot shows 25 ng of Recombinant Human Leptin R Fc Chimera (Catalog # 389-LR) and Recombinant Mouse Leptin R Fc Chimera (Catalog # 497-LR). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human Leptin R Monoclonal Antibody (Catalog # MAB867) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Leptin R at approximately 155-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Human whole blood monocytes were stained with Human Leptin R Monoclonal Antibody (Catalog # MAB867, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).

Leptin R was detected in immersion fixed paraffin-embedded sections of human hypothalamus using Mouse Anti-Human Leptin R Monoclonal Antibody (Catalog # MAB867) at 1.7 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface of neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: 52263
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Leptin R Antibody (52263) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Leptin R
  Phe22-Asp839 (predicted)
  Accession # P48357
• Specificity: Detects human Leptin R in direct ELISAs and Western blots. In Western blots, cross reactivity with recombinant mouse Leptin R is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: LEPR
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 1.7-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Leptin R Antibody (52263) [Unconjugated]

• B219
• CD295 antigen
• CD295
• DB
• DKFZp686B1731
• huB219
• LEPR
• LEP-R
• Leptin R
• leptin receptor
• LeptinR
• OB R
• OB receptor
• OB-R
• OBRCD295

Background

Leptin receptor (OB-R), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OB-R, including a long form (OB-R_L) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OB-R_s) lacking signal-transducing capabilities, have been identified. The extracellular domains of the short and long forms of OB-R are identical. An OB-R transcript, lacking a transmembrane domain and potentially encoding a soluble form of the receptor, has also been described. OB-R_L transcripts were reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. Expression of OB-R_s transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney, and primitive hematopoietic cell populations. OB-R has been shown to be encoded by the mouse diabetes (db) and rat fatty (fa) genes. Rodents homozygous for the db or fa mutations have been known to exhibit an obesity phenotype. Human OB-R long form encodes a 1165 amino acid (aa) precursor protein with a 22 aa signal peptide, an 819 aa extracellular domain, a 21 aa transmembrane domain and a 303 aa cytoplasmic domain. The extracellular domain of OB-R contain two hemopoietin receptor domains, a fibronectin type III domain and the WSXWS domain. Recombinant soluble OB-R has been shown to bind Leptin with high affinity and is a potent Leptin antagonist.

1. Tartaglia, L.A. et al. (1995) Cell 83:1263.
2. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
3. Tartaglia, L.A. (1997) J. Biol. Chem. 272:6093.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Leptin R Antibody (MAB867)(6)

Publications using MAB867

• Y Chen, H Wang, Q Yang, W Zhao, Y Chen, Q Ni, W Li, J Shi, W Zhang, L Li, Y Xu, H Zhang, D Miao, L Xing, W Sun Single-cell RNA landscape of the osteoimmunology microenvironment in periodontitis Theranostics, 2022-01-01;12(3):1074-1096. 2022-01-01 [PMID: 35154475] (Flow Cytometry, IHC, Human)
• K Watanabe, M Suzukawa, S Arakawa, K Kobayashi, S Igarashi, H Tashimo, H Nagai, S Tohma, T Nagase, K Ohta Leptin enhances cytokine/chemokine production by normal lung fibroblasts by binding to leptin receptor Allergol Int, 2019-04-24;0(0):. 2019-04-24 [PMID: 31029506] (Flow Cytometry, Human)
• D. Törőcsik, D. Kovács, E. Camera, M. Lovászi, K. Cseri, G.G. Nagy et al. Leptin promotes a proinflammatory lipid profile and induces inflammatory pathways in human SZ95 sebocytes British Journal of Dermatology 2014-12-01 [PMID: 24975960]
• Liang G, Gao W, Liang A Normal leptin expression, lower adipogenic ability, decreased leptin receptor and hyposensitivity to Leptin in Adolescent Idiopathic Scoliosis. PLoS ONE, 2012-05-15;7(5):e36648. 2012-05-15 [PMID: 22615788] (ICC, Western Blot, Human)
• Hongo S, Watanabe T, Arita S Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages. Am. J. Physiol. Endocrinol. Metab., 2009-08-01;297(2):E474-82. 2009-08-01 [PMID: 19625677] (Western Blot, Human)
• Francois F, Roper J, Goodman AJ, Pei Z, Ghumman M, Mourad M, de Perez AZ, Perez-Perez GI, Tseng CH, Blaser MJ The association of gastric leptin with oesophageal inflammation and metaplasia. Gut, 2007-08-30;57(1):16-24. 2007-08-30 [PMID: 17761783] (IHC-P, Human)

Review for Leptin R Antibody (MAB867) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Craig Dorrell Flow Human 12/06/2016

Summary

• Application: Flow Cytometry
• Sample Tested: Human pancreatic islet cells
• Species: Human
• Lot: DAQ071610A

Bioinformatics

• Gene Symbol: LEPR
• Uniprot:
  • P48357()