Leptin R Antibody (52263) [Fluorescein]

Human peripheral blood monocytes were stained with Mouse Anti-Human Leptin R Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB867F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 52263
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

Leptin R Antibody (52263) [Fluorescein] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Leptin R
  Phe22-Asp839 (predicted)
  Accession # P48357
• Specificity: Detects human Leptin R in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse Leptin R is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: LEPR
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  12 months from date of receipt, 2 to 8 degreesC as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Leptin R Antibody (52263) [Fluorescein]

• B219
• CD295 antigen
• CD295
• DB
• DKFZp686B1731
• huB219
• LEPR
• LEP-R
• Leptin R
• leptin receptor
• LeptinR
• OB R
• OB receptor
• OB-R
• OBRCD295

Background

Leptin Receptor (OB-R), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity to gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OB-R, including a long form (OB-R L ) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OB-R s ) lacking signal-transducing capabilities, have been identified. The extracellular domains of the short and long forms of OB-R are identical. An OB-R transcript, lacking a transmembrane domain and encoding a potentially soluble form of the receptor has also been described. OB-R L transcripts were reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. Expression of OB-R s transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney, and primitive hematopoietic cell populations. OB-R has been shown to be encoded by the mouse diabetes (db) and rat fatty (fa) genes. Rodents homozygous for db or fa mutations have been known to exhibit an obesity phenotype. Human OB-R long form encodes a 1165 amino acid (aa) precursor protein with a 22 aa signal peptide, an 819 aa extracellular domain, a 21 aa transmembrane domain and a 303 aa cytoplasmic domain. The extracellular domain of OB-R contains two hemopoietin receptor domains, a fibronectin type III domain and the WSXWS domain. Recombinant soluble OB-R has been shown to bind Leptin with high affinity and is a potent Leptin antagonist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Leptin R Antibody (FAB867F)(3)

Publications using FAB867F

• Michaela Tencerova, Lilli Lundby, Steen Buntzen, Stig Norderval, Helene Tarri Hougaard, Bodil Ginnerup Pedersen et al. Molecular differences of adipose-derived mesenchymal stem cells between non-responders and responders in treatment of transphincteric perianal fistulas Stem Cell Research & Therapy 2021-12-01 [PMID: 34819138]
• I Bähr, J Jahn, A Zipprich, I Pahlow, J Spielmann, H Kielstein Impaired natural killer cell subset phenotypes in human obesity Immunol. Res., 2018-04-01;0(0):. 2018-04-01 [PMID: 29560551] (Flow Cytometry, Human)
• Carbone F, De Rosa V, Carrieri P, Montella S, Bruzzese D, Porcellini A, Procaccini C, La Cava A, Matarese G Regulatory T cell proliferative potential is impaired in human autoimmune disease. Nat Med, 2013-12-08;20(1):69-74. 2013-12-08 [PMID: 24317118] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: LEPR
• Uniprot:
  • P48357()