Recombinant Rat Leptin/OB Protein

Product Discontinued

Recombinant Rat Leptin/OB Protein Summary

• Description: Recombinant biologically active Leptin/OB protein.

  Source:E. coli

  Amino Acid Sequence: Ala-Val-Pro-Ile-His

• Preparation Method: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.201 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a calibrated solution of rat Leptin a Reference Standard.
• Details of Functionality: Biological activity of rat Leptin is evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: LEP
• Purity: >95%, by HPLC
• Endotoxin Note: Less than 0.1 ng/ug (IEU/ug) ofrHuLeptin.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a concentrated solution with 0.0045mM NaHCO3.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by HPLC
• Reconstitution Instructions: Reconstitute with sterilized 18 M omega-cm water to a final concentration of at least 0.1 mg/ml.

Notes

After reconstitution, store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Alternate Names for Recombinant Rat Leptin/OB Protein

• FLJ94114
• LEP
• leptin (murine obesity homolog)
• leptin (obesity homolog, mouse)
• Leptin
• OB
• Obese protein
• obese, mouse, homolog of
• Obesity factor
• OBOBS

Background

A peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: LEP