Leptin/OB Antibody (4F8)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
4F8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Leptin/OB Antibody (4F8) Summary

Immunogen
Recombinant full length protein.
Localization
Secreted
Specificity
This recognizes recombinant human leptin and leptin circulating in human blood.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LEP
Purity
Protein A purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
Application Notes
ELISA and Western Blot: This antibody was tested in sandwich ELISA as capture and detection antibodies with human blood samples. This antibody detects antigen in human blood with high sensitivity. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species, but may be cross reactive to mouse and rat due to 85% and 84% identity to human leptin respectively.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Alternate Names for Leptin/OB Antibody (4F8)

  • FLJ94114
  • LEP
  • leptin (murine obesity homolog)
  • leptin (obesity homolog, mouse)
  • Leptin
  • OB
  • Obese protein
  • obese, mouse, homolog of
  • Obesity factor
  • OBOBS

Background

Leptin (from the Greek leptos, meaning thin) is a protein hormone with important effects in regulating body weight, metabolism and reproductive function. The protein is approximately 16 kDa in mass and encoded by the obese (ob) gene. Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells. Leptin's effects on body weight are mediated through effects on hypothalamic centers that control feeding behavior and hunger, body temperature and energy expenditure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol LEP
Entrez