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LEDGF Antibody (6E4)

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Western Blot: LEDGF Antibody (6E4) [NBP2-37646] - Western blot analysis using PSIP1 mouse mAb against HepG2 (1), Jurkat (2), K562 (3), Cos7 (4), PC-12 (5), Hela (6), and NIH/3T3 (7) cell lysate.
Western Blot: LEDGF Antibody (6E4) [NBP2-37646] - Western blot analysis using PSIP1 mouse mAb against HepG2 (1), Jurkat (2), K562 (3), Cos7 (4), PC-12 (5), Hela (6), and NIH/3T3 (7) cell lysate.
Immunocytochemistry/ Immunofluorescence: LEDGF Antibody (6E4) [NBP2-37646] - Immunofluorescence analysis of NIH/3T3 cells using PSIP1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 ...read more
Immunohistochemistry: LEDGF Antibody (6E4) [NBP2-37646] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and ovarian cancer tissues (right) using PSIP1 mouse mAb with DAB staining.
Immunohistochemistry: LEDGF Antibody (6E4) [NBP2-37646] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and brain tissues (right) using PSIP1 mouse mAb with DAB staining.
ELISA: LEDGF Antibody (6E4) [NBP2-37646] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity Hu, Rt, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
6E4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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LEDGF Antibody (6E4) Summary

Immunogen
Purified recombinant fragment of human PSIP1 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PSIP1
Purity
Unpurified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
LEDGF Knockout 293T Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for LEDGF Antibody (6E4)

  • CLL-associated antigen KW-7
  • Dense fine speckles 70 kDa protein
  • DFS 70
  • DFS70
  • LEDGF
  • LEDGFDFS 70
  • Lens epithelium-derived growth factor
  • MGC74712
  • p52
  • p75
  • PAIP
  • PC4 and SFRS1 interacting protein 1
  • PC4 and SFRS1 interacting protein 2
  • PC4 and SFRS1-interacting protein
  • PSIP1
  • PSIP2
  • transcriptional coactivator p52/p75
  • Transcriptional coactivator p75/p52

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Knockout Validated: c-Fos Antibody (2H2) [NBP2-50037] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and c-Fox knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Mouse Anti-Human c-Fox Monoclonal Antibody (Catalog # NBP2-50037) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for c-Fox at approximately 52 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: c-Fos Antibody (2H2) [NBP2-50037] - Analysis of cell lysates using mouse c-Fos mAb, dilution 1:1,000 (Green), and rabbit GAPDH pAb, dilution 1:20,000 (Red) used as a loading control. [1] protein standard (red), [2] HeLa cells in serum free media. [3] HeLa cells stimulated with 20% fetal bovine serum for 2hrs after 36hrs in serum free media. [4] rat cortical neurons. [5] rat cortical neurons treated with membrane depolarization buffer for 5hrs. Multiple bands at 50-65kDa in stimulated or treated cell lysates correspond to different forms of the c-Fos proten. The single band at 37 kDa represents GAPDH protein.
Immunocytochemistry/Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Section of rat hippocampus stained with mouse monoclonal antibody to c-FOS NBP2-50037 in red and counterstained with rabbit polyclonal antibody to FOX3/NeuN. DAPI reveals nuclei of neurons and glia in blue. The hippocampal neurons stain green for FOX3/NeuN and a few also are expressing c-FOS, and so appear orange. These cells were spontaneously active at the time the animal was sacrificed.
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Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.
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Control Lysate(s)

LEDGF Knockout 293T Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PSIP1
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