LEDGF Antibody (6E4)

Western Blot: LEDGF Antibody (6E4) [NBP2-37646] - Western blot analysis using PSIP1 mouse mAb against HepG2 (1), Jurkat (2), K562 (3), Cos7 (4), PC-12 (5), Hela (6), and NIH/3T3 (7) cell lysate.

Immunocytochemistry/Immunofluorescence: LEDGF Antibody (6E4) [NBP2-37646] - Immunofluorescence analysis of NIH/3T3 cells using PSIP1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry: LEDGF Antibody (6E4) [NBP2-37646] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and ovarian cancer tissues (right) using PSIP1 mouse mAb with DAB staining.

Immunohistochemistry: LEDGF Antibody (6E4) [NBP2-37646] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and brain tissues (right) using PSIP1 mouse mAb with DAB staining.

ELISA: LEDGF Antibody (6E4) [NBP2-37646] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Discontinued

LEDGF Antibody (6E4) Summary

• Immunogen: Purified recombinant fragment of human PSIP1 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PSIP1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 75 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for LEDGF Antibody (6E4)

• CLL-associated antigen KW-7
• Dense fine speckles 70 kDa protein
• DFS 70
• DFS70
• LEDGF
• LEDGFDFS 70
• Lens epithelium-derived growth factor
• MGC74712
• p52
• p75
• PAIP
• PC4 and SFRS1 interacting protein 1
• PC4 and SFRS1 interacting protein 2
• PC4 and SFRS1-interacting protein
• PSIP1
• PSIP2
• transcriptional coactivator p52/p75
• Transcriptional coactivator p75/p52

Background

The human p75 protein is a non TAF transcription coactivator that mediates activator dependent transcription by RNA polymerase II in vitro through most tested activators. Although p75 and p52 are derived from alternatively splicing of a single gene and share most coding sequence, they reveal different function in several aspects. In addition to functioning as a transcription coactivator, p75 has been reported to be involved in regulating growth of epithelial cells, pathogenesis of atopic dermatitis and activity of HIV1 integrase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSIP1