LC3 Antibody Pack

Images

 
Western Blot: LC3 Antibody Pack [NB910-40435] - Detection of autophagic LC3 in mouse ES cell lysate using NB 100-2331. The atg5-/- lane (ES cells, cultured to form embryonic bodies, that are deficient in conversion of ...read more
Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Brain, Cerebral Cortex, Cell Processes in Gray Matter 40x.
Western Blot: LC3 Antibody Pack [NB910-40435] - Detection of LC3 in human brain lysate using NB 100-2220. Atg5-/- ES cells from Dr. Noboru Mizushima [Mizushima, N. et al. J. Cell Biol. 152 (2001)] Photo courtesy of Dr. ...read more
Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Brain, Cerebral Cortex, Neurons with Cell Processes 40x.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC

Order Details

LC3 Antibody Pack Summary

Marker
Autophagosome Marker
Gene
MAP1LC3B

Applications/Dilutions

Reviewed Applications
Read 2 Reviews rated 5
using
NB910-40435 in the following application:

Publications
Read Publications using
NB910-40435 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Kit Components

Notes

1 vial each of: NB100-2220 (0.1ml) and NB100-2331 (0.1ml).

Background

LC3, a mammalian homologue of Apg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. However, LC3 is now thought to also be involved in autophagy. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. LC3-II is the first mammalian protein identified that specifically associates with the autophagosome membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

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Publications for LC3 Antibody Pack (NB910-40435)(2)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(1)
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Filter By Species
Rat
(1)
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Reviews for LC3 Antibody Pack (NB910-40435) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.
We have 2 reviews tested in 2 application: WB, IHC.

Reviews using NB910-40435:
Filter by Applications
WB
(1)
IHC
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot LC3 NB910-40435
Enlarge
reviewed by:
Anonymous
WB Mouse 07/11/2014
View

Summary

ApplicationWestern Blot
SpeciesMouse
LotS-2
Pub Med ID24614889
FileView PDF
Immunohistochemistry LC3 NB910-40435
Enlarge
reviewed by:
Anonymous
IHC Mouse 04/29/2014
View

Summary

ApplicationImmunohistochemistry
Sample TestedHuman Brain
SpeciesMouse
LotNB100-2331
CommentsFormalin fixed(fresh cut). Experimental steps are being evaluated

Secondary Antibody

Secondary DescriptionGoat anti-Mouse
Secondary Manufacturer Cat#Vactor BA-9200
Secondary Concentration1:600

Comments

CommentsFormalin fixed(fresh cut). Experimental steps are being evaluated

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for LC3 Antibody Pack (NB910-40435). (Showing 1 - 1 of 1 FAQs).

  1. Do you have a suggestion regarding the starting point for this antibody's dilution in Western blot?
    • We recommend starting with 2.0ug/ml when using this antibody in Western blot.

Additional LC3 Products

LC3 NB910-40435

Bioinformatics Tool for LC3 Antibody Pack (NB910-40435)

Discover related pathways, diseases and genes to LC3 Antibody Pack (NB910-40435). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for LC3 Antibody Pack (NB910-40435)

Discover more about diseases related to LC3 Antibody Pack (NB910-40435).
 

Pathways for LC3 Antibody Pack (NB910-40435)

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PTMs for LC3 Antibody Pack (NB910-40435)

Learn more about PTMs related to LC3 Antibody Pack (NB910-40435).

Blogs on LC3. Showing 1-10 of 19 blog posts - Show all blog posts.

Key Targets in Apoptosis, Necroptosis, and Autophagy
Cell death/recycling pathways such as apoptosis, necroptosis, and autophagy are an integral part of the growth, development, homeostasis as well as the pathophysiology in the life of living organisms. These signaling pathways are highly regulated and...  Read full blog post.

Required proteins for p62/SQSTM1 regulation and a role for p62/SQSTM1 in neuronal autophagy
Autophagy is a crucial cellular process that clears the cell of protein aggregates, toxins, and damaged cell products. Accumulation of toxins, damaged cell products and unwanted proteins has been proven to play a role in aging and many forms of dis...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

Three things everyone studying autophagy should know
Novus Biologicals' antibodies are the gold standard to monitor autophagy and detect LC3 expression. The recently published Guidelines for the Use and Interpretation of Assays for Monitoring Autophagy (3rd Edition) comprehensively details methods...  Read full blog post.

WIPI1 - An essential regulator of early autophagosome assembly
WD repeat domain phosphoinositide-interacting protein 1 (WIPI) is involved in the lysosomal degradation of cytoplasmic components during starvation-induced autophagy. WIPI1 is a seven bladed beta-propeller protein that provides a scaffold for the ...  Read full blog post.

ATG4C - A regulator of the early steps of autophagosome assembly
Autophagy is an important cellular process that maintains homeostasis by degrading and recycling damaged proteins and organelles. Autophagy receptors, such as p62/SQSTM1, recognize these intracellular cargo and mediate their engulfment by the doubl...  Read full blog post.

CHOP/GADD153 - A regulator and marker for ER-stress induced apoptosis
C/EBP homologous protein (CHOP) is a transcription factor that regulates apoptosis in response to cellular stress. CHOP also known as growth arrest and DNA damage 153 (GADD153) was first cloned because of its induction in response to genotoxic str...  Read full blog post.

ATG4D - A regulator of autophagy and apoptosis
Autophagy is an essential cellular process whereby damaged proteins and organelles are degraded and recycled. Autophagy, while happening constantly at a basal level, is tightly regulated and can be further induced under cellular stress. One of the...  Read full blog post.

Beclin 2, a mammal-specific homolog of Beclin 1 with unique functional similarities and differences
Beclin 2 (BECN2) is also called Beclin-1-like protein 1/ BECN1P1 and it was recently identified by He et al 2013 as a mammal-specific homolog of the evolutionarily conserved protein Beclin 1 which is well established for its role in the regulation...  Read full blog post.

p62/SQSTM1 - targeting ubiquitinated proteins for autophagic degradation
During autophagy ubiquitinated cargo or substrates are engulfed in a double-membrane autophagosome and transported to the lysosome for degradation. This process is important for maintaining cellular homeostasis and for degrading damaged organelles...  Read full blog post.

Showing 1-10 of 19 blog posts - Show all blog posts.

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Contact Information

Product PDFs

Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
07/11/2014
Application: WB
Species: Mouse

Anonymous
04/29/2014
Application: IHC
Species: Mouse

Bioinformatics

Gene Symbol MAP1LC3B