LC3 Antibody Pack

Images

 
Biological Strategies: Knockout Validated: LC3 Antibody Pack [NB910-40435] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 ...read more
Biological Strategies: Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Rapamycin increases autophagy in brains of PDAPP mice. Representative epifluorescent (c200x) image of hippocampal CA1 in control- and ...read more
Genetic Strategies: Knockout Validated: LC3 Antibody Pack [NB910-40435] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using ...read more
Biological Strategies: Western Blot: LC3 Antibody Pack [NB910-40435] - High autophagosome concentration is consumed during early immortalized human mesenchymal stem cell differentiation. Immortalized human ...read more
Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Localization of LC3 by immunohistochemical method. The immunohistochemical staining shows that LC3, CRF, and HIF-1alpha have an overlapping localization in villous ...read more
Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Brain, Cerebral Cortex, Cell Processes in Gray Matter 40x.
Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Analysis in PFA fixed NIH/3T3 cells using anti-LC3A antibody. Image from verified customer review. LC3A Antibody [NB100-2331]

Product Details

Summary
Reactivity Hu, Mu, FiSpecies Glossary
Applications WB, IHC, KO

Order Details

LC3 Antibody Pack Summary

Immunogen
For NB100-2331: A synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25-121) [Uniprot: Q9H492].
For NB100-2220: Polyclonal LC3B Antibody was made to a synthetic peptide made to an N-terminal portion of the human LC3B protein sequence (between residues 1-100) [UniProt# Q9GZQ8].
Marker
Autophagosome Marker
Gene
MAP1LC3B

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Knockout Validated
  • Western Blot
Application Notes
See individual datasheets for validated applications.
Reviewed Applications
Read 3 Reviews rated 5
using
NB910-40435 in the following applications:

Publications
Read Publications using
NB910-40435 in the following applications:

  • 3 publications
  • IHC
    2 publications
  • WB
    9 publications

Reactivity Notes

Fish reactivity reported in scientific literature (PMID:32818499).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Kit Components

Notes

This pack contains 1 vial each of: NB100-2220 (0.1 mL) and NB100-2331 (0.1 mL).

Alternate Names for LC3 Antibody Pack

  • ATG8F
  • LC3B
  • MAP1A/1BLC3
  • map1lc3b
  • MAP1LC3B-a
  • microtubule associated protein 1 light chain 3 beta

Background

Autophagy (macroautophagy) is a catabolic process which targets intracellular components such as proteins and organelles for degradation. Originally described as a bulk degradation process, current research supports its selective nature (1). Selective autophagy targets specific cellular components for degradation including the endoplasmic reticulum (2) (ER-phagy), mitochondria3 (mitophagy), peroxisomes (3) (pexophagy), ribosomes (4) (ribophagy) and bacteria (5) (xenophagy). Autophagy relies on a newly formed phagophore, a membrane structure which elongates, sequesters cellular content, and fuses to form a double membrane vesicle known as the autophagosome. Fusion of autophagosomes with lysosomes gives rise to the autophagolysosome, where cellular components are degraded by lysosome hydrolases (1).

Autophagic flux is supported by autophagy-related proteins (Atgs) initially identified in yeast (6,7). The core autophagy machinery is comprised of 17 Atg proteins that play specific roles in autophagosome formation. Among these Atg proteins, Atg8 is not only involved in autophagosome formation but also functions in cargo selection. In mammals, several Atg8 homologues have been identified including microtubule-associated protein 1 light chain 3 alpha, beta and gamma - LC3A, LC3B, and LC3C (8) respectively, as well as GABA type A receptor-associated protein (GABARAP), GABARAP-Like1, and GABARAP-Like2 (9). LC3 (predicted molecular weight 14kD) is ubiquitously expressed and undergoes posttranslational processing after synthesis. First, the cysteine protease Atg4 cleaves a carboxy terminal sequence to generate the cytosolic form LC3-I. Next, E1-like (Atg7) and E2-like (Atg3) enzymes conjugate phosphatidylethanolamine to the newly exposed carboxyterminal glycine, generating LC3-II. Finally, the Atg12-Atg5-Atg16L1 complex participates in LC3 lipidation and autophagosome formation (10). LC3B-I to LC3B-II conversion correlates with autophagosome number and is considered the best marker to monitor autophagy.

References

1. Yu, L., Chen, Y., & Tooze, S. A. (2018). Autophagy pathway: Cellular and molecular mechanisms. Autophagy. https://doi.org/10.1080/15548627.2017.1378838

2. Forrester, A., De Leonibus, C., Grumati, P., Fasana, E., Piemontese, M., Staiano, L., ... Settembre, C. (2019). A selective ER-phagy exerts procollagen quality control via a Calnexin-FAM 134B complex. The EMBO Journal. https://doi.org/10.15252/embj.201899847

3. He, X., Zhu, Y., Zhang, Y., Geng, Y., Gong, J., Geng, J., ... Zhong, H. (2019). RNF34 functions in immunity and selective mitophagy by targeting MAVS for autophagic degradation. The EMBO Journal. https://doi.org/10.15252/embj.2018100978

4. Mathai, B., Meijer, A., & Simonsen, A. (2017). Studying Autophagy in Zebrafish. Cells. https://doi.org/10.3390/cells6030021

5. Losier, T. T., Akuma, M., McKee-Muir, O. C., LeBlond, N. D., Suk, Y., Alsaadi, R. M., ... Russell, R. C. (2019). AMPK Promotes Xenophagy through Priming of Autophagic Kinases upon Detection of Bacterial Outer Membrane Vesicles. Cell Reports. https://doi.org/10.1016/j.celrep.2019.01.062

6. Nakatogawa, H., Suzuki, K., Kamada, Y., & Ohsumi, Y. (2009). Dynamics and diversity in autophagy mechanisms: Lessons from yeast. Nature Reviews Molecular Cell Biology. https://doi.org/10.1038/nrm2708

7. Tsukada, M., & Ohsumi, Y. (1993). Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae. FEBS Letters. https://doi.org/10.1016/0014-5793(93)80398-E

8. Wild, P., McEwan, D. G., & Dikic, I. (2014). The LC3 interactome at a glance. Journal of Cell Science. https://doi.org/10.1242/jcs.140426

9. Igloi, G. L. (2001). Cloning, expression patterns, and chromosome localization of three human and two mouse homologues of GABAA receptor-associated protein. Genomics. https://doi.org/10.1006/geno.2001.6555

10. Glick, D., Barth, S., & Macleod, K. F. (2010). Autophagy: Cellular and molecular mechanisms. Journal of Pathology. https://doi.org/10.1002/path.2697

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-2331
Species: Am, Ca, Fi, Hu, Mu, Pl, Rt, Ze
Applications: ChIP, ELISA, Flow, IB, ICC/IF, IHC-WhMt, IHC, IHC-Fr, IHC-P, IP, Simple Western, SB, WB
H00008878-M01
Species: Bv, Ha, Hu, Mu, Rb, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NB500-249
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, Simple Western, WB
NBP2-82090
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP1-55202
Species: Hu
Applications: IHC, IHC-P, WB
H00002965-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, KD, S-ELISA, WB
NBP1-31381
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP2-37399
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-02627
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-19151
Species: Bv, Hu, Ma
Applications: ICC/IF, IHC, IHC-P, IP, WB
NB600-607
Species: Hu, Rt
Applications: ELISA, IHC, IHC-P, NULL, WB
NB100-56708
Species: Ch, ChHa, Hu, Ma, Mu, Po, Rt
Applications: CyTOF-ready, EM, Flow, HEStain, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, KD, KO, Simple Western, WB
MAB6608
Species: Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow, Simple Western, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NB110-53818
Species: Al, Bv, Dr, Fi, Gp, Hu, Mu, Po, Pm, Rt, Xp, Ze
Applications: EM, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP, KD, KO, PLA, RIA, Simple Western, WB
NBP2-24709
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NB600-1335
Species: Hu, Mu, Pm, Rt
Applications: ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-P, IP, KD, Simple Western, WB
NB100-56098
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
NBP3-03280
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB910-40435
Species: Hu, Mu, Fi
Applications: WB, IHC, KO

Publications for LC3 Antibody Pack (NB910-40435)(14)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Fish.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(3)
IHC
(2)
WB
(9)
All Applications
Filter By Species
Human
(4)
Mouse
(5)
Rat
(4)
Fish
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB910-40435 Applications Species
Wang Y, Zhao H, Nie X et al. Zinc application alleviates the adverse renal effects of arsenic stress in a protein quality control way in common carp Environ. Res. Aug 17 2020 [PMID: 32818499] (WB, Fish) WB Fish
Wang SL, Shao BZ, Zhao SB et al. Intestinal autophagy links psychosocial stress with gut microbiota to promote inflammatory bowel disease Cell Death Dis [PMID: 31564717] (WB, Mouse) WB Mouse
Ye B, Wang Q, Hu H et al. Restoring autophagic flux attenuates cochlear spiral ganglion neuron degeneration by promoting TFEB nuclear translocation via inhibiting MTOR Autophagy Feb 1 2019 [PMID: 30706760] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Wu A, Hu P, Lin J et al. Activating Cannabinoid Receptor 2 Protects Against Diabetic Cardiomyopathy Through Autophagy Induction. BMC Cancer. Nov 15 2018 [PMID: 30459625] (WB, Mouse) WB Mouse
Kang I, Lee BC, Lee JY et al. Stem cell-secreted 14,15- epoxyeicosatrienoic acid rescues cholesterol homeostasis and autophagic flux in Niemann-Pick-type C disease. Arch Med Sci. Oct 1 2018 [PMID: 30429460] (WB, Mouse) WB Mouse
Xie Q, Liu M, Yan YF et al. Exogenous tetranectin protects against MPP+-induced neurotoxicity by inhibiting apoptosis and autophagy through p70S6K1 World Neurosurg Oct 17 2018 [PMID: 30342268] (WB, Human) WB Human
Xie W, Zheng W, Liu M et al. BRF1 ameliorates LPS-induced inflammation through autophagy crosstalking with MAPK/ERK signaling Genes Dis Sep 1 2018 [PMID: 30320187] (ICC/IF, Mouse) ICC/IF Mouse
Song R, Yang Y, Lei H et al. HDAC6 inhibition protects cardiomyocytes against doxorubicin-induced acute damage by improving a-tubulin acetylation J. Mol. Cell. Cardiol. Oct 10 2018 [PMID: 30315806] (WB, Rat) WB Rat
An Q, Yan W, Zhao Y, Yu K. Enhanced neutrophil autophagy and increased concentrations of IL-6, IL-8, IL-10 and MCP-1 in rheumatoid arthritis Int. Immunopharmacol. Oct 9 2018 [PMID: 30312880] (IHC, ICC/IF, Human) IHC, ICC/IF Human
Bao J, Shi Y, Tao M et al. Pharmacological inhibition of autophagy by 3-MA attenuates hyperuricemic nephropathy Clin. Sci. Oct 7 2018 [PMID: 30293967] (WB, Rat) WB Rat
Show All 14 Publications.

Reviews for LC3 Antibody Pack (NB910-40435) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using NB910-40435:
Filter by Applications
ICC
(1)
WB
(1)
IHC
(1)
All Applications
Filter by Species
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Christiana Doulami
ICC Mouse 02/12/2019
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman cell
SpeciesMouse
LotNB100-2341
Western Blot LC3 NB910-40435
Enlarge
5
reviewed by:
Verified Customer
WB Mouse 07/11/2014
View

Summary

ApplicationWestern Blot
SpeciesMouse
LotS-2
Pub Med ID24614889
FileView PDF
Immunohistochemistry LC3 NB910-40435
Enlarge
5
reviewed by:
Verified Customer
IHC Mouse 04/29/2014
View

Summary

ApplicationImmunohistochemistry
Sample TestedHuman Brain
SpeciesMouse
LotNB100-2331
CommentsFormalin fixed(fresh cut). Experimental steps are being evaluated

Secondary Antibody

Secondary DescriptionGoat anti-Mouse
Secondary Manufacturer Cat#Vactor BA-9200
Secondary Concentration1:600

Comments

CommentsFormalin fixed(fresh cut). Experimental steps are being evaluated

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for LC3 Antibody Pack (NB910-40435). (Showing 1 - 1 of 1 FAQs).

  1. Do you have a suggestion regarding the starting point for this antibody's dilution in Western blot?
    • We recommend starting with 2.0ug/ml when using this antibody in Western blot.

Additional LC3 Products

Array NB910-40435

Bioinformatics Tool for LC3 Antibody Pack (NB910-40435)

Discover related pathways, diseases and genes to LC3 Antibody Pack (NB910-40435). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for LC3 Antibody Pack (NB910-40435)

Discover more about diseases related to LC3 Antibody Pack (NB910-40435).
 

Pathways for LC3 Antibody Pack (NB910-40435)

View related products by pathway.

PTMs for LC3 Antibody Pack (NB910-40435)

Learn more about PTMs related to LC3 Antibody Pack (NB910-40435).

Blogs on LC3. Showing 1-10 of 40 blog posts - Show all blog posts.

Autophagy and Metastasis
  By Christina Towers, PhD The majority of cancer patients die from metastatic disease at secondary sites. The threshold to undergo metastasis is high. Only a minority of cancer cells acquire invasive phenotypes...  Read full blog post.

Optogenetic Control of Mitophagy: AMBRA1 based mitophagy switch
By Christina Towers, PhD Mitophagy in the BrainSelective autophagic degradation of damaged mitochondria, known as mitophagy, has been described as a cyto-protective process. Accordingly, defects in mitophagy h...  Read full blog post.

Autophagic flux: Is p62 a good indicator?
By Christina Towers, PhD Is p62 a good indicator of autophagic flux? The short answer: Yes … but … SQSTM1 encodes the cargo adaptor protein, p62, which interacts with autophagic substrates and delivers them to aut...  Read full blog post.

Best genes to knockout to control autophagic flux
By Christina Towers, PhD Autophagy flux: Basic principlesAutophagic flux is defined as the amount of cellular material degraded and recycled through the process of autophagy, whereby cells break down and disca...  Read full blog post.

Make each cell count: How to assess autophagy using flow cytometry
Kristy R. Howell, PhDThe cellular recycling process known as autophagy may be induced by a variety of conditions including reduced nutrient availability, serum starvation and pharmacological agents (e.g., Rapamyci...  Read full blog post.

How to visualize autophagy by microscopy
By Christina Towers, PhD Autophagy is a recycling process that relies on the formation of a unique organelle termed an autophagosome. An elegant way to monitor autophagy is through various microscopy techniques to...  Read full blog post.

Microglia: pruning shears for homeostatic maintenance in the brain
By Jennifer Sokolowski, MD, PhD.Microglia play a critical role in pruning neurons and synapses during homeostatic maintenance in the adult brain.1 A recent study by Ayata et al. (2018) identified regional differe...  Read full blog post.

The LC3 A, B, C’s and 1, 2, 3’s
By Christina Towers, PhD Autophagy is a catabolic process used to breakdown and recycle damaged proteins and organelles. It is a multistep process that, in its simplest form, consists of 4 steps: initiation, phago...  Read full blog post.

Best way to quantitatively measure Autophagic Flux
By Christina Towers, PhD Autophagy is a stress-induced cellular recycling process that plays an important physiological role in many diseases. It is induced by a variety of stimuli, both intracellular and extracel...  Read full blog post.

Best Methods to Induce and Inhibit Autophagy Pharmacologically
By Christina Towers, PhD Autophagy facilitates the degradation and recycling of damaged cytoplasmic material. The multistep process includes a double membrane structure called an autophagosome that engulfs pr...  Read full blog post.

Showing 1-10 of 40 blog posts - Show all blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

 
Christiana Doulami
02/12/2019
Application: ICC
Species: Mouse

Verified Customer
07/11/2014
Application: WB
Species: Mouse

Verified Customer
04/29/2014
Application: IHC
Species: Mouse

Bioinformatics

Gene Symbol MAP1LC3B