LASS6 Antibody - BSA Free

Western Blot: LASS6 Antibody [NBP1-76965] - Rat brain tissue lysate with LASS6 antibody at (A) 1 and (B) 2 ug/ml.

Western Blot: LASS6 Antibody [NBP1-76965] - CerS6 (LASS6)-dependent C16-ceramide generation mediates stress response to serum starvation. Serum withdrawal elevates CerS6 in cultured cells with concomitant increase of p53 and p21 while CerS6 siRNA silencing prevents the p53 and p21 accumulation in response to serum withdrawal. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-018-06650-y) licensed under a CC-BY license.

Immunohistochemistry: LASS6 Antibody - BSA Free [NBP1-76965] - Immunohistochemistry of LASS6 in mouse brain tissue with LASS6 antibody at 2.5 ug/mL.

Immunocytochemistry/ Immunofluorescence: LASS6 Antibody - BSA Free [NBP1-76965] - Immunofluorescence of LASS6 in Mouse Brain cells with LASS6 antibody at 5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

LASS6 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit LASS6 Antibody - BSA Free (NBP1-76965) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-LASS6 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 13 amino acid synthetic peptide near the carboxy terminus of the human LASS6. The immunogen is located within the last 50 amino acids of LASS6. Amino Acid Squence: DSEPPGKNPHTAT
• Specificity: At least two isoforms of LASS6 are known to exist. This antibody is predicted not to cross-react with LASS5.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CERS6
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Knockdown Validated
  • Western Blot 1-2 ug/mL
• Control Peptide:

  LASS6 Peptide (NBP1-76965PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for LASS6 Antibody - BSA Free

• CerS6
• LAG1 homolog, ceramide synthase 6
• LAG1 longevity assurance homolog 6 (S. cerevisiae)
• LAG1 longevity assurance homolog 6
• longevity assurance homolog 6
• MGC129949
• MGC129950

Background

The LASS (longevity assurance homolog) family members represent a subgroup of the homeobox gene family and are highly conserved from yeasts to mammals. Six members of this family of proteins have been characterized (LASS1-6) and all are involved in ceramide synthesis during cell growth regulation and cancer differentiation. Like the highly homologous LASS5, LASS6 is also an endoplasmic reticulum, multi-pass membrane protein. LASS6 is also involved in the synthesis of C14, C16 and C18-ceramide, but shows a preference for unsaturated fatty acids. LASS6 is broadly expressed in a wide range of tissues and microarray data suggests that it may play a role in cancer differentiation and early embryonic development. At least two isoforms of LASS6 are known to exist. This antibody is predicted not to cross-react with LASS5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LASS6 Antibody (NBP1-76965)(1)

Publication using NBP1-76965

• Fekry B, Jeffries kA, Esmaeilniakooshkghazi A et al. C16-ceramide is a natural regulatory ligand of p53 in cellular stress response. Nat Commun. 2018-10-08 [PMID: 30297838] (WB, Human)

Bioinformatics

• Gene Symbol: CERS6
• Uniprot:
  • AAI09285(Human)