LASS3 Antibody - BSA Free

Immunohistochemistry-Paraffin: LASS3 Antibody [NBP1-84536] - Staining of human Skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: LASS3 Antibody [NBP1-84536] - Staining of human skin shows weak to moderate nuclear positivity in keratinocytes.

Immunohistochemistry-Paraffin: LASS3 Antibody [NBP1-84536] - Staining of human testis shows weak to moderate nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: LASS3 Antibody [NBP1-84536] - Staining of human cervix shows very weak nuclear postivity in squamous epithelial cells.

Staining of human cell line U-251 MG shows localization to nucleoplasm.

• Reactivity: Hu
• Applications: ICC/IF, IHC, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

LASS3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit LASS3 Antibody - BSA Free (NBP1-84536) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-LASS3 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: FVASPLAKSFGIKETVRKVTPNTVLENFFKHSTRQPLQTDIYGLAKKCNLTERQVERWFRSR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CERS3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
  
  Although previous lots of this product were validated for use in Western Blot, this product is no longer validated for use in that application.
• Control Peptide:

  LASS3 Protein (NBP1-84536PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for LASS3 Antibody - BSA Free

• CerS3
• LAG1 homolog, ceramide synthase 3
• LAG1 longevity assurance homolog 3 (S. cerevisiae)
• LAG1 longevity assurance homolog 3
• MGC27091

Background

LASS3 may be involved in sphingolipid synthesis or its regulation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LASS3 Antibody (NBP1-84536)(2)

Publications using NBP1-84536

• Radner FP, Marrakchi S, Kirchmeier P et al. Mutations in CERS3 Cause Autosomal Recessive Congenital Ichthyosis in Humans. PLoS Genet 2013-06-01 [PMID: 23754960]
• Thakoersing VS, van Smeden J, Mulder AA et al. Increased presence of monounsaturated Fatty acids in the stratum corneum of human skin equivalents. J Invest Dermatol 2013-01-01 [PMID: 22895362]

Bioinformatics

• Gene Symbol: CERS3