Langerin/CD207 Antibody - BSA Free

Western Blot: Langerin/CD207 Antibody [NB100-56733] - Analysis of langerin (CD207) in mouse lung tissue lysate using 2 ug/ml of langerin antibody. Lane 1, Without blocking peptide; Lane 2, With blocking peptide.

Immunohistochemistry-Paraffin: Langerin/CD207 Antibody [NB100-56733] - Human tonsil tissue. Heat mediated antigen retrieval in citrate buffer, 95C for 30 min. Image from verified customer review.

Immunohistochemistry-Paraffin: Langerin/CD207 Antibody [NB100-56733] - Tissue microarray from normal mouse organs (NBP2-30225) using rabbit polyclonal Langerin/CD207 antibody at 5ug/ml concentration. The IHC-P protocol involved antigen retrieval with 10mM sodium citrate buffer pH 6.0 and this antibody generated specific staining of the Langerhans cells in mouse ear lobe sections.

Immunohistochemistry-Paraffin: Langerin/CD207 Antibody [NB100-56733] - Tissue microarray from normal mouse organs (NBP2-30225) using rabbit polyclonal Langerin/CD207 antibody at 5ug/ml concentration. The IHC-P protocol involved antigen retrieval with 10mM sodium citrate buffer pH 6.0 and this antibody generated specific staining of the Langerhans cells in mouse ear lobe sections.

Immunohistochemistry-Paraffin: Langerin/CD207 Antibody [NB100-56733] - Analysis of langerin (CD207) in mouse breast tissue using an isotype control antibody (left) and langerin (CD207) antibody (right) at 5 ug/ml.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Langerin/CD207 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Langerin/CD207 Antibody - BSA Free (NB100-56733) is a polyclonal antibody validated for use in IHC, WB and Flow. Anti-Langerin/CD207 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Amino acids 26-44 of mouse Langerin were used as immunogen (Uniprot # Q8VBX4)
• Predicted Species: Rat (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CD207
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular) reported in scientific literature (PMID 18369922)
  • Flow Cytometry 1:20-1:2000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Frozen 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1 - 3 ug/ml
• Application Notes: A protein band of ~36 kDa is detected in mouse lung tissue lysate. Pre-incubation with the immunizing peptide completely removes langerin reactivity from NB100-56733.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Langerin/CD207 Antibody - BSA Free

• CD207 antigen
• CD207 antigen, langerin
• CD207 molecule, langerin
• CD207
• CLEC4KLangerhans cell specific c-type lectin
• C-type lectin domain family 4 member K
• C-type lectin domain family 4, member K
• Langerin

Background

Langerin (CD207) is a type II membrane-associated C-type lectin known to be expressed exclusively by Langerhans cells. Langerin recognizes mannose residues via its single carbohydrate recognition domain (CRD). Langerin is localized not only on the cell surface, but also intracellularly in close association with Birbeck granules. Transfection of Langerin cDNA into fibroblasts creates a compact network of membrane structures with typical features of Birbeck granules (BG). Langerin is thus a potent inducer of membrane superimposition and zippering leading to BG formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Langerin/CD207 Antibody (NB100-56733)(5)

Publications using NB100-56733

• Yasuda T Ura T Taniguchi M, Yoshida H. Intradermal Delivery of Antigens Enhances Specific IgG and Diminishes IgE Production: Potential Use for Vaccination and Allergy Immunotherapy. PLoS ONE. 2016-12-15 [PMID: 27973543] (IF/IHC, Mouse)
• Legge KL, Schlueter AJ. Dendritic cells in chronic in vivo ethanol exposure models. Methods Mol Biol. 2008-01-01 [PMID: 18369922] (Flow - IC, Mouse)
  
  Details:
  Flow (intracellular), mouse dentritic cells, Fig. 4.
• Ness KJ, Fan J, Wilke WW et al. Chronic ethanol consumption decreases murine Langerhans cell numbers and delays migration of Langerhans cells as well as dermal dendritic cells. Alcohol Clin Exp Res. 2008-04-01 [PMID: 18241312] (Flow - IC, Mouse)
  
  Details:
  Flow (intracellular), mouse lymph node cells, Figs. 4A, 5A.
• Dajee M, Muchamuel T, Schryver B et al. Blockade of experimental atopic dermatitis via topical NF-kappaB decoy oligonucleotide. J Invest Dermatol. 2006-08-01 [PMID: 16628194] (IHC-Fr)
• Sung SS, Fu SM, Rose CE Jr et al. A major lung CD103 (alphaE)-beta7 integrin-positive epithelial dendritic cell population expressing Langerin and tight junction proteins. J Immunol. 2006-02-15 [PMID: 16455972] (IF/IHC)

Reviews for Langerin/CD207 Antibody (NB100-56733) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Santhosh Sivajothi IHC-P Human 09/25/2018

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Tonsil tissue
• Species: Human
• Lot: 1110729A-08

reviewed by: Verified Customer IHC-P Mouse 05/04/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: mouse skin
• Species: Mouse
• Lot: 12230729A-05

FAQs for Langerin/CD207 Antibody (NB100-56733). (Showing 1 - 2 of 2 FAQs).

1. I’m contacting you because I’m using often your Antibodies and I would like to conjugate some of them by myself. For that purpose I would need to have Antibodies in a buffer solution that do not contains any additive like NaAz or BSA. Could you please tell me if it is possible to order such antibodies, and if there is a minimum volume quantity to order?
   • Typically there is a minimum order quantity for custom formats of our products (1mg on average). However we are transitioning to offering more of our products in an azide/BSA free format. Please let us know which products are needed, and we can check the feasibility of offering them in the desired format.
2. Would you please recommend a dilution for this application? Do you have any suggested protocol for IHC?-EndFragment-->
   • For IHC-P we recommend a dilution range of 1:100-1:500. The lab used the following protocol

Bioinformatics

• Gene Symbol: CD207
• Entrez:
  • 246278(Mouse)
  • 502852(Rat)
• Uniprot:
  • Q8VBX4(Mouse)