Langerin/CD207 Antibody

Images

 
Western Blot: Langerin/CD207 Antibody [NB100-56733] - Analysis of langerin (CD207) in mouse lung tissue lysate using 2 ug/ml of langerin antibody. Lane 1, Without blocking peptide; Lane 2, With blocking peptide.
Immunohistochemistry-Paraffin: Langerin/CD207 Antibody [NB100-56733] - Analysis of langerin (CD207) in paraffin-embedded formalin-fixed mouse breast tissue using an isotype control antibody (left) and langerin (CD207) ...read more
Immunohistochemistry-Paraffin: Langerin/CD207 Antibody [NB100-56733] - IHC analysis of a formalin fixed paraffin embedded tissue microarray from normal mouse organs (NBP2-30225) using rabbit polyclonal Langerin/CD207 ...read more
Immunohistochemistry-Paraffin: Langerin/CD207 Antibody [NB100-56733] - IHC analysis of a formalin fixed paraffin embedded tissue microarray from normal mouse organs (NBP2-30225) using rabbit polyclonal Langerin/CD207 ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Langerin/CD207 Antibody Summary

Immunogen
Amino acids 26-44 of mouse Langerin were used as immunogen (Uniprot # Q8VBX4)
Predicted Species
Rat (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD207
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Flow Cytometry 1:20-1:2000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Frozen 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
A protein band of ~36 kDa is detected in mouse lung tissue lysate. Pre-incubation with the immunizing peptide completely removes langerin reactivity from NB100-56733.
Publications
Read Publications using
NB100-56733 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Langerin/CD207 Antibody

  • CD207 antigen
  • CD207 antigen, langerin
  • CD207 molecule, langerin
  • CD207
  • CLEC4KLangerhans cell specific c-type lectin
  • C-type lectin domain family 4 member K
  • C-type lectin domain family 4, member K
  • Langerin

Background

Langerin (CD207) is a type II membrane-associated C-type lectin known to be expressed exclusively by Langerhans cells. Langerin recognizes mannose residues via its single carbohydrate recognition domain (CRD). Langerin is localized not only on the cell surface, but also intracellularly in close association with Birbeck granules. Transfection of Langerin cDNA into fibroblasts creates a compact network of membrane structures with typical features of Birbeck granules (BG). Langerin is thus a potent inducer of membrane superimposition and zippering leading to BG formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Langerin/CD207 Antibody (NB100-56733)(5)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: Flow - IC, IHC, IHC-Fr.


Filter By Application
Flow - IC
(2)
IHC
(2)
IHC-Fr
(1)
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Filter By Species
Mouse
(3)
All Species

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Product General Protocols

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WB Video Protocol

FAQs for Langerin/CD207 Antibody (NB100-56733). (Showing 1 - 1 of 1 FAQs).

  1. I’m contacting you because I’m using often your Antibodies and I would like to conjugate some of them by myself. For that purpose I would need to have Antibodies in a buffer solution that do not contains any additive like NaAz or BSA. Could you please tell me if it is possible to order such antibodies, and if there is a minimum volume quantity to order?
    • Typically there is a minimum order quantity for custom formats of our products (1mg on average). However we are transitioning to offering more of our products in an azide/BSA free format. Please let us know which products are needed, and we can check the feasibility of offering them in the desired format.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD207
Entrez
Uniprot