DEC-205/CD205 Antibody (MG38) - BSA Free

Flow Cytometry: CD205 Antibody (MG38) [NBP2-00328] - Staining of normal human peripheral blood cells (left) and mature dendritic cells (right) with Mouse IgG2b kappa Isotype Control Purified (open histogram) or 0.015 ug of Anti-Human CD205 Purified (filled histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE . Total viable cells were used for analysis.

• Reactivity: Hu
• Applications: Flow, IHC, IP
• Clone: MG38
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

DEC-205/CD205 Antibody (MG38) - BSA Free Summary

• Description: Novus Biologicals Mouse DEC-205/CD205 Antibody (MG38) - BSA Free (NBP2-00328) is a monoclonal antibody validated for use in IHC, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Clone MG38 was used by HLDA to establish CD designation.
• Immunogen: The immunogen for this antibody is CD205 (DEC-205).
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: LY75
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
• Application Notes: This MG38 antibody has been tested by flow cytometric analysis of in vitro derived human dendritic cells and peripheral blood leukocytes. This can be used at less than or equal to 0.03 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The MG38 antibody has also been reported for use in immunoprecipitation and immunohistochemical staining of frozen tissue sections. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for DEC-205/CD205 Antibody (MG38) - BSA Free

• CD205
• CLEC13B
• C-type lectin domain family 13 member B
• DEC205
• DEC-205
• GP200-MR6
• Ly75
• Ly-75
• Lymphocyte antigen 75

Background

The MG38 monoclonal antibody reacts with human CD205, also known as DEC-205, an integral membrane protein homologous to the macrophage mannose receptor. CD205 is an endocytic dendritic receptor that mediates efficient processing and presentation of antigens on MHC class II productions in vivo. CD205 is highly expressed by dendritic cells (DCs) within the T cell areas of lymphoid tissues, particularly on CD8+ DCs that are believed to play a role in cross-priming. Expression of CD205 is detected with this antibody at high intensity on in vitro derived mature human DCs (mDC) and at intermediate intensity on immature human DCs (iDC). All leukocytes from peripheral blood show a degree of staining with this mAb that can be minimized by using very low concentrations of MG38.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LY75