LAMP-1/CD107a Antibody (LY1C6)

Images

 
Western Blot: LAMP-1/CD107a Antibody (LY1C6) [NB100-1952] - analysis of Rat liver microsome lysate showing detection of LAMP1 protein using Mouse Anti-LAMP1 Monoclonal Antibody, Clone Ly1C6. Load: 15 ug protein. Block: ...read more
Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody (LY1C6) [NB100-1952] - analysis using Mouse Anti-LAMP1 Monoclonal Antibody, Clone Ly1C6. Tissue: transfected HeLa cells. Species: Human. Primary Antibody: ...read more
Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody (LY1C6) [NB100-1952] - CHO K1 cells incubated with LAMP-1/CD107a, at 1:100 and goat anti-mouse Alexa 488 secondary antibody (Image provided by Dr. Guy ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, EM, ICC/IF, IP
Clone
LY1C6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

LAMP-1/CD107a Antibody (LY1C6) Summary

Immunogen
Rat liver lysosomal membrane preparations detects an approx. 120kDa protein corresponding to the molecular mass of LAMP1 on SDS PAGE immunoblots.
Localization
Cell Membrane, Endosome, Lysosome, Endosome membrane, Lysosome membrane
Marker
Late Endosome Marker
Specificity
Detects approx 120kDa.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LAMP1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Electron Microscopy 1:10-1:500
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunoprecipitation 1:10-1:500
Application Notes
1 ug/ml was sufficient for detection of LAMP1 in rat liver miscrosome by ECL immunoblot analysis.
Publications
Read Publication using NB100-1952.

Reactivity Notes

Based on homology, It's predicted to detect Human, Mouse and Hamster.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for LAMP-1/CD107a Antibody (LY1C6)

  • CD107 antigen-like family member A
  • CD107a antigen
  • CD107a
  • LAMP1
  • LAMP-1
  • LAMPA
  • LGP120
  • lysosomal-associated membrane protein 1
  • lysosome-associated membrane glycoprotein 1
  • Lysosome-associated membrane protein 1

Background

Lysosome-associated membrane proteins (LAMP1), also known as lgp120 or lgpA, is a type 1 integral membrane protein that is transported from trans-Golgi network to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboxyl-terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1. This mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMC to the site of inflammation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LAMP-1/CD107a Antibody (NB100-1952)(1)


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-1952AF405
Alexa Fluor 488 Labeled NB100-1952AF488
Alexa Fluor 647 Labeled NB100-1952AF647
Alexa Fluor 700 Labeled NB100-1952AF700
DyLight 350 Labeled NB100-1952UV
DyLight 405 Labeled NB100-1952V
DyLight 488 Labeled NB100-1952G
DyLight 680 Labeled NB100-1952FR
DyLight 755 Labeled NB100-1952IR

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Bioinformatics

Gene Symbol LAMP1
Entrez
Uniprot