LAMP-1/CD107a Antibody (H4A3)

Images

 
Western Blot: LAMP-1/CD107a Antibody (H4A3) [NBP2-25183] - Human peripheral blood cells.
Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody (H4A3) [NBP2-25183] - HeLa cells.
Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (H4A3) [NBP2-25183] - Human kidney.
Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) [NBP2-25183] - An intracellular stain was performed on THP-1 cells with LAMP-1/CD107a antibody (H4A3) NBP2-25183PE (blue) and a matched isotype control NBP2-27287PE ...read more
Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) [NBP2-25183] - Surface staining of human peripheral blood cells with anti-CD107a (H4A3) PE.

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
Clone
H4A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.00 mg/ml

LAMP-1/CD107a Antibody (H4A3) Summary

Immunogen
Human PBMC
Marker
Late Endosome Marker
Specificity
The mouse monoclonal antibody H4A3 recognizes CD107a, an approximately 100-120 kDa glycoprotein expressed mainly on lysosomal, but also on the plasma membrane.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LAMP1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1;10-1;2000
  • Immunohistochemistry 1;10-1;20000
  • Immunohistochemistry-Frozen 1:10-1:5000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • CyTOF-ready

Reactivity Notes

Non-Human Primates,. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 um filter sterilized.
Preservative
No Preservative
Concentration
1.00 mg/ml
Purity
Protein A purified

Alternate Names for LAMP-1/CD107a Antibody (H4A3)

  • CD107 antigen-like family member A
  • CD107a antigen
  • CD107a
  • LAMP1
  • LAMP-1
  • LAMPA
  • LGP120
  • lysosomal-associated membrane protein 1
  • lysosome-associated membrane glycoprotein 1
  • Lysosome-associated membrane protein 1

Background

CD107a (lysosome-associated membrane protein-1, LAMP-1), together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. Increased CD107a immunoreactivity is observed in neurones, and in glial cells surrounding senile plaques in Alzheimers disease cases and is localized mainly in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. CD107a is a good marker of mast cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LAMP-1/CD107a Antibody (NBP2-25183) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-25183AF405
Alexa Fluor 488 Labeled NBP2-25183AF488
Alexa Fluor 647 Labeled NBP2-25183AF647
Alexa Fluor 700 Labeled NBP2-25183AF700
Allophycocyanin Labeled NBP2-25183APC
Biotin Labeled NBP2-25183B
DyLight 350 Labeled NBP2-25183UV
DyLight 405 Labeled NBP2-25183V
DyLight 488 Labeled NBP2-25183G
DyLight 550 Labeled NBP2-25183R
DyLight 650 Labeled NBP2-25183C
DyLight 680 Labeled NBP2-25183FR
DyLight 755 Labeled NBP2-25183IR
HRP Labeled NBP2-25183H
PE Labeled NBP2-25183PE
PerCP Labeled NBP2-25183PCP

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Bioinformatics

Gene Symbol LAMP1
Entrez