LAMP-1/CD107a Antibody (5H6) - BSA Free

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5H6) [NBP2-25154] - Analysis of HeLa cells stained with mouse LAMP-1/CD107a mAb, dilution 1:500 (Red), and costained with chicken Vimentin pAb, dilution 1:10000, (Green). DAPI staining of nuclear DNA (Blue). The cells were treated with 50uM of chloroquine, an inhibitor of autophagy, for 16hrs prior to staining. The LAMP-1/CD107a antibody reveals vesicular staining of LAMP1 protein accumulated in swollen lysosomes, while the Vimentin antibody specifically labels the intermediate filament network in these cells.

Western Blot: LAMP-1/CD107a Antibody (5H6) [NBP2-25154] - Analysis of different cell lysates using mouse mAb to LAMP1, NBP2-25154 dilution 1:10,000 in green. Cells were maintained under normal conditions (Ct), or treated with 50uM of chloroquine (CQ), an inhibitor of autophagy, for 24 hours: [1] protein standard (red), [2] HeLa Ct, [3] HeLa+CQ, [4] NIH-3T3 Ct, and [5] NIH-3T3+CQ. The smeared band between 75-120kDa corresponds to variably glycosylated forms of the LAMP1 protein detected only in the human cells, this antibody fails to recognize the rodent homologue. The same blot was probed with chicken pAB to HSP60, dilution 1:20,000. The HSP60 antibody reveals heat shock 60 protein with apparent molecular weight of 60kDa in all preparations.

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5H6) [NBP2-25154] - D2.0 R cells activate autophagy upon entering a dormant state. Representative images are shown for immunofluorescent staining of lysosomal (LAMP-1/CD107a, red) and autophagic (LC3, green) markers of D2.0 R cells in BME (upper panels) and BME + COL1 (lower panels). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-04070-6), licensed under a CC-BY license.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clone: 5H6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

LAMP-1/CD107a Antibody (5H6) - BSA Free Summary

• Immunogen: Amino acids 32-350 of the human LAMP1 precursor sequence. [UniProt# P11279]
• Localization: Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Late endosome.
• Marker: Late Endosome Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAMP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:2000
  • Immunohistochemistry 1:2000
  • Western Blot 1:1000-1:10000
• Application Notes: This LAMP1 (5H6) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, and Western Blot, where a band can be seen at ~90-120 kDa.
• Theoretical MW: 90-120 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human Specific. Mouse reactivity reported in scientific literature (PMID: 29789598).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% glycerol
• Preservative: 5mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LAMP-1/CD107a Antibody (5H6) - BSA Free

• CD107 antigen-like family member A
• CD107a antigen
• CD107a
• LAMP1
• LAMP-1
• LAMPA
• LGP120
• lysosomal-associated membrane protein 1
• lysosome-associated membrane glycoprotein 1
• Lysosome-associated membrane protein 1

Background

LAMP-1 (lysosome-associated membrane protein 1), also known as CD107a (cluster of differentiation 107a), is a major component of lysosomal membranes that plays an important role in lysosomal biogenesis, autophagy, and cholesterol metabolism (1). LAMP-1 is a type I transmembrane glycoprotein that is expressed on plasma membranes and the membranes of endosomes, autolysosomes, and lysosomes (1,2). Additionally, LAMP-1/CD107a is a commonly used marker for natural killer (NK) cell degranulation (3). LAMP-1 and another lysosomal-associated membrane protein, LAMP-2, together make up about half of all lysosome membrane proteins (1). Additionally, LAMP-1 has a role in presenting carbohydrate ligands to selectins (2). Human LAMP-1 protein is comprised of 417 amino acids (aa) with a theoretical molecular weight of 44.8 kDa; however, glycosylation can increase the molecular weight upwards of 120 kDa (1, 4). Structurally, LAMP-1 protein contains a large luminal/extracellular domain (29-382 aa), a helical transmembrane domain (383-405 aa), and a short cytoplasmic tail (406-417 aa) (1,2). Additionally, the protein has many N- and O-linked glycosylation sites which helps with stability in the membrane (1,2).

LAMP-1 plays an important role in autophagy-mediated ATP-release during apoptosis where lysosomes containing intracellular ATP migrate to the plasma membrane and, during exocytosis, LAMP-1 is exposed to the cell surface (5). Studies have found that knockdown of LAMP-1 blocks the ATP release from the cell (5). Furthermore, an absence of LAMP-1 and LAMP-2 leads to an accumulation of lysosomal cholesterol (6). Lysosomal membrane dysfunction or defects has also been associated with disease development (6,7). For example, one feature of pancreatitis is autophagy impairment which is caused by lysosomal dysfunction and a corresponding decrease in lysosomal-membrane associated proteins LAMP-1 and LAMP-2 (7).

References

1. Eskelinen E. L. (2006). Roles of LAMP-1 and LAMP-2 in lysosome biogenesis and autophagy. Molecular aspects of medicine, 27(5-6), 495-502. https://doi.org/10.1016/j.mam.2006.08.005

2. Cheng, X. T., Xie, Y. X., Zhou, B., Huang, N., Farfel-Becker, T., & Sheng, Z. H. (2018). Revisiting LAMP1 as a marker for degradative autophagy-lysosomal organelles in the nervous system. Autophagy, 14(8), 1472-1474. https://doi.org/10.1080/15548627.2018.1482147

3. Krzewski, K., & Coligan, J. E. (2012). Human NK cell lytic granules and regulation of their exocytosis. Frontiers in immunology, 3, 335. https://doi.org/10.3389/fimmu.2012.00335

4. Uniprot (P11279)

5. Wang, Y., Martins, I., Ma, Y., Kepp, O., Galluzzi, L., & Kroemer, G. (2013). Autophagy-dependent ATP release from dying cells via lysosomal exocytosis. Autophagy, 9(10), 1624-1625. https://doi.org/10.4161/auto.25873

6. Schwake, M., Schr0der, B., & Saftig, P. (2013). Lysosomal membrane proteins and their central role in physiology. Traffic (Copenhagen, Denmark), 14(7), 739-748. https://doi.org/10.1111/tra.12056

7. Gukovsky, I., Pandol, S. J., Mareninova, O. A., Shalbueva, N., Jia, W., & Gukovskaya, A. S. (2012). Impaired autophagy and organellar dysfunction in pancreatitis. Journal of gastroenterology and hepatology, 27 Suppl 2(Suppl 2), 27-32. https://doi.org/10.1111/j.1440-1746.2011.07004.x

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LAMP-1/CD107a Antibody (NBP2-25154)(6)

Publications using NBP2-25154

• Lampinen, R, R Mitochondrial dysfunction in non-neuronal cells in Alzheimer's disease Thesis 2022-01-01
• Niskanen J, Peltonen S, Ohtonen S et al. Uptake of alpha-synuclein preformed fibrils is suppressed by inflammation and induces an aberrant phenotype in human microglia. Glia 2024-10-22 [PMID: 39435593]
• Vera-Ramirez L, Vodnala SK, Nini R et al. Autophagy promotes the survival of dormant breast cancer cells and metastatic tumour recurrence Nat Commun 2018-05-22 [PMID: 29789598] (ICC/IF, Mouse)
• Keatinge M, Gegg ME, Watson L et al. Acid sphingomyelinase inhibition results in profound rescue of neuronal function in glucocerebrosidase deficiency unexpected opposing effects of Parkinson disease risk genes bioRxiv 2020-01-01 (Zebrafish)
• Jiang W, Liu F, Li H et al. TREM2 ameliorates anesthesia and surgery-induced cognitive impairment by regulating mitophagy and NLRP3 inflammasome in aged C57/BL6 mice Neurotoxicology [PMID: 35447280] (IHC-P, Mouse)
• Sozen E, Demirel-Yalciner T, Sari D Et al. Deficiency of SREBP1c modulates autophagy mediated lipid droplet catabolism during oleic acid induced steatosis Metabolism open 2021-12-01 [PMID: 34704008] (ICC/IF, Mouse)

FAQs for LAMP-1/CD107a Antibody (NBP2-25154). (Showing 1 - 1 of 1 FAQs).

1. I am currently interested in developing a CD107a degranulation assay with sheep cells. I have been looking at your anti-CD107a antibodies that react with bovine samples (ref NBP2-25155 and NBP2-25154), as anti-bovine antibodies can cross react with sheep samples. I have seen on your webpage that both antibodies have been tested in WB and in immunofluorescence. I was wondering whether you have tested them for flow cytometry as it is the application we are intending to use in the lab. I was also wondering whether you could send us a sample of either antibody so that we can test them in sheep and for this application.
   • Neither of these have been tested in FLOW. We do have a great progam that will allow you to try these antibodies in any species or application that has not been tested and if you share your results with us you will earn a voucher for the cost of the product for a future puchase. This is called the innovators reward. If you would like to test an antibody in an untested species and/or application and share your results with us, I can recommend our Innovator's Reward™ program. Our Innovator’s Reward™ program was created to allow researchers the opportunity to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply submit an online review detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. As such we do not provide free samples. I apologize for this.

Bioinformatics

• Gene Symbol: LAMP1
• Entrez:
  • 3916(Human)
• OMIM:
  • 153330(Human)
• Uniprot:
  • P11279()