LAMP-1/CD107a Antibody (5E7) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5E7) - Azide and BSA Free [NBP2-80824] - MCF-7 human breast cancer cell line. Secondary antibody goat anti-mouse IgG Fc Dy680 (NBP1-72880). Image from verified customer review. Image from the standard format of this antibody.

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (5E7) - Azide and BSA Free [NBP2-80824] - Analysis of FFPE human kidney tissue section using LAMP-1 antibody (clone 5E7) at 1:100 dilution. The antibody generated a specific cytoplasmic staining (with dotted appearance) in all cells, especially the ductal and tubular epithelial cells. Few cells f

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5E7) - Azide and BSA Free [NBP2-80824] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-LAMP (5E7) at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5E7) - Azide and BSA Free [NBP2-80824] - ICC usage of NBP2-52721. Image submittied via verified customer review. Image from the standard format of this antibody.

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (5E7) - Azide and BSA Free [NBP2-80824] - Analysis of FFPE human kidney tissue section using LAMP-1 antibody (clone 5E7) at 1:100 dilution. The antibody generated a specific cytoplasmic staining (with dotted appearance) in all the ductal and tubular epithelial cells. Image from the standard forma

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: 5E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

LAMP-1/CD107a Antibody (5E7) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse LAMP-1/CD107a Antibody (5E7) - Azide and BSA Free (NBP2-52721) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Partial recombinant protein made to an internal portion of the human LAMP1 protein (between amino acids 200-400) [UniProt P11279]
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAMP1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 2.0 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for LAMP-1/CD107a Antibody (5E7) - Azide and BSA Free

• CD107 antigen-like family member A
• CD107a antigen
• CD107a
• LAMP1
• LAMP-1
• LAMPA
• LGP120
• lysosomal-associated membrane protein 1
• lysosome-associated membrane glycoprotein 1
• Lysosome-associated membrane protein 1

Background

LAMP-1 (lysosome-associated membrane protein 1), also known as CD107a (cluster of differentiation 107a), is a major component of lysosomal membranes that plays an important role in lysosomal biogenesis, autophagy, and cholesterol metabolism (1). LAMP-1 is a type I transmembrane glycoprotein that is expressed on plasma membranes and the membranes of endosomes, autolysosomes, and lysosomes (1,2). Additionally, LAMP-1/CD107a is a commonly used marker for natural killer (NK) cell degranulation (3). LAMP-1 and another lysosomal-associated membrane protein, LAMP-2, together make up about half of all lysosome membrane proteins (1). Additionally, LAMP-1 has a role in presenting carbohydrate ligands to selectins (2). Human LAMP-1 protein is comprised of 417 amino acids (aa) with a theoretical molecular weight of 44.8 kDa; however, glycosylation can increase the molecular weight upwards of 120 kDa (1, 4). Structurally, LAMP-1 protein contains a large luminal/extracellular domain (29-382 aa), a helical transmembrane domain (383-405 aa), and a short cytoplasmic tail (406-417 aa) (1,2). Additionally, the protein has many N- and O-linked glycosylation sites which helps with stability in the membrane (1,2).

LAMP-1 plays an important role in autophagy-mediated ATP-release during apoptosis where lysosomes containing intracellular ATP migrate to the plasma membrane and, during exocytosis, LAMP-1 is exposed to the cell surface (5). Studies have found that knockdown of LAMP-1 blocks the ATP release from the cell (5). Furthermore, an absence of LAMP-1 and LAMP-2 leads to an accumulation of lysosomal cholesterol (6). Lysosomal membrane dysfunction or defects has also been associated with disease development (6,7). For example, one feature of pancreatitis is autophagy impairment which is caused by lysosomal dysfunction and a corresponding decrease in lysosomal-membrane associated proteins LAMP-1 and LAMP-2 (7).

References

1. Eskelinen E. L. (2006). Roles of LAMP-1 and LAMP-2 in lysosome biogenesis and autophagy. Molecular aspects of medicine, 27(5-6), 495-502. https://doi.org/10.1016/j.mam.2006.08.005

2. Cheng, X. T., Xie, Y. X., Zhou, B., Huang, N., Farfel-Becker, T., & Sheng, Z. H. (2018). Revisiting LAMP1 as a marker for degradative autophagy-lysosomal organelles in the nervous system. Autophagy, 14(8), 1472-1474. https://doi.org/10.1080/15548627.2018.1482147

3. Krzewski, K., & Coligan, J. E. (2012). Human NK cell lytic granules and regulation of their exocytosis. Frontiers in immunology, 3, 335. https://doi.org/10.3389/fimmu.2012.00335

4. Uniprot (P11279)

5. Wang, Y., Martins, I., Ma, Y., Kepp, O., Galluzzi, L., & Kroemer, G. (2013). Autophagy-dependent ATP release from dying cells via lysosomal exocytosis. Autophagy, 9(10), 1624-1625. https://doi.org/10.4161/auto.25873

6. Schwake, M., Schr0der, B., & Saftig, P. (2013). Lysosomal membrane proteins and their central role in physiology. Traffic (Copenhagen, Denmark), 14(7), 739-748. https://doi.org/10.1111/tra.12056

7. Gukovsky, I., Pandol, S. J., Mareninova, O. A., Shalbueva, N., Jia, W., & Gukovskaya, A. S. (2012). Impaired autophagy and organellar dysfunction in pancreatitis. Journal of gastroenterology and hepatology, 27 Suppl 2(Suppl 2), 27-32. https://doi.org/10.1111/j.1440-1746.2011.07004.x

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LAMP1