LAMP-1/CD107a Antibody (5E7) - BSA Free

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - ICC usage of NBP2-52721. Image submittied via verified customer review.

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - Analysis of FFPE human kidney tissue section using LAMP-1 antibody (clone 5E7) at 1:100 dilution. The antibody generated a specific cytoplasmic staining (with dotted appearance) in all the ductal and tubular epithelial cells.

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-LAMP (5E7) at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - MCF-7 human breast cancer cell line. Secondary antibody goat anti-mouse IgG Fc Dy680 (NBP1-72880). Image from verified customer review.

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - Analysis of FFPE human kidney tissue section using LAMP-1 antibody (clone 5E7) at 1:100 dilution. The antibody generated a specific cytoplasmic staining (with dotted appearance) in all cells, especially the ductal and tubular epithelial cells. Few cells from the glomeruli showed nuclear signal also.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: 5E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

LAMP-1/CD107a Antibody (5E7) - BSA Free Summary

• Immunogen: Partial recombinant protein made to an internal portion of the human LAMP1 protein (between amino acids 200-400) [UniProt P11279]
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAMP1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 5 ug/mL
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 2.0 ug/mL

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for LAMP-1/CD107a Antibody (5E7) - BSA Free

• CD107 antigen-like family member A
• CD107a antigen
• CD107a
• LAMP1
• LAMP-1
• LAMPA
• LGP120
• lysosomal-associated membrane protein 1
• lysosome-associated membrane glycoprotein 1
• Lysosome-associated membrane protein 1

Background

LAMP-1 (lysosome-associated membrane protein 1), also known as CD107a (cluster of differentiation 107a), is a major component of lysosomal membranes that plays an important role in lysosomal biogenesis, autophagy, and cholesterol metabolism (1). LAMP-1 is a type I transmembrane glycoprotein that is expressed on plasma membranes and the membranes of endosomes, autolysosomes, and lysosomes (1,2). Additionally, LAMP-1/CD107a is a commonly used marker for natural killer (NK) cell degranulation (3). LAMP-1 and another lysosomal-associated membrane protein, LAMP-2, together make up about half of all lysosome membrane proteins (1). Additionally, LAMP-1 has a role in presenting carbohydrate ligands to selectins (2). Human LAMP-1 protein is comprised of 417 amino acids (aa) with a theoretical molecular weight of 44.8 kDa; however, glycosylation can increase the molecular weight upwards of 120 kDa (1, 4). Structurally, LAMP-1 protein contains a large luminal/extracellular domain (29-382 aa), a helical transmembrane domain (383-405 aa), and a short cytoplasmic tail (406-417 aa) (1,2). Additionally, the protein has many N- and O-linked glycosylation sites which helps with stability in the membrane (1,2).

LAMP-1 plays an important role in autophagy-mediated ATP-release during apoptosis where lysosomes containing intracellular ATP migrate to the plasma membrane and, during exocytosis, LAMP-1 is exposed to the cell surface (5). Studies have found that knockdown of LAMP-1 blocks the ATP release from the cell (5). Furthermore, an absence of LAMP-1 and LAMP-2 leads to an accumulation of lysosomal cholesterol (6). Lysosomal membrane dysfunction or defects has also been associated with disease development (6,7). For example, one feature of pancreatitis is autophagy impairment which is caused by lysosomal dysfunction and a corresponding decrease in lysosomal-membrane associated proteins LAMP-1 and LAMP-2 (7).

References

1. Eskelinen E. L. (2006). Roles of LAMP-1 and LAMP-2 in lysosome biogenesis and autophagy. Molecular aspects of medicine, 27(5-6), 495-502. https://doi.org/10.1016/j.mam.2006.08.005

2. Cheng, X. T., Xie, Y. X., Zhou, B., Huang, N., Farfel-Becker, T., & Sheng, Z. H. (2018). Revisiting LAMP1 as a marker for degradative autophagy-lysosomal organelles in the nervous system. Autophagy, 14(8), 1472-1474. https://doi.org/10.1080/15548627.2018.1482147

3. Krzewski, K., & Coligan, J. E. (2012). Human NK cell lytic granules and regulation of their exocytosis. Frontiers in immunology, 3, 335. https://doi.org/10.3389/fimmu.2012.00335

4. Uniprot (P11279)

5. Wang, Y., Martins, I., Ma, Y., Kepp, O., Galluzzi, L., & Kroemer, G. (2013). Autophagy-dependent ATP release from dying cells via lysosomal exocytosis. Autophagy, 9(10), 1624-1625. https://doi.org/10.4161/auto.25873

6. Schwake, M., Schr0der, B., & Saftig, P. (2013). Lysosomal membrane proteins and their central role in physiology. Traffic (Copenhagen, Denmark), 14(7), 739-748. https://doi.org/10.1111/tra.12056

7. Gukovsky, I., Pandol, S. J., Mareninova, O. A., Shalbueva, N., Jia, W., & Gukovskaya, A. S. (2012). Impaired autophagy and organellar dysfunction in pancreatitis. Journal of gastroenterology and hepatology, 27 Suppl 2(Suppl 2), 27-32. https://doi.org/10.1111/j.1440-1746.2011.07004.x

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LAMP-1/CD107a Antibody (NBP2-52721)(7)

Publications using NBP2-52721

• Chen Q, Cao Y, Li H et al. Sodium nitroprusside alleviates nanoplastics-induced developmental toxicity by suppressing apoptosis, ferroptosis and inflammation Journal of environmental management 2023-08-01 [PMID: 37536135] (WB, Zebrafish)
• Alban TJ, Grabowski MM, Otvos B et al. The MIF promoter SNP rs755622 is associated with immune activation in glioblastoma JCI insight 2023-07-10 [PMID: 37252795] (IHC-P, Human)
  
  Details:
  1:50 IHC-P dilution
• Larionova I, Kiselev A, Kazakova E et al. Tumor-associated macrophages respond to chemotherapy by detrimental transcriptional reprogramming and suppressing stabilin-1 mediated clearance of EGF Frontiers in immunology 2023-03-07 [PMID: 36960065] (ICC/IF, Human)
  
  Details:
  Dilution used in IHC-P 1:100
• Zhang G, Cong Y, Liu FL et al. A nanomaterial targeting the spike protein captures SARS-CoV-2 variants and promotes viral elimination Nature nanotechnology 2022-08-22 [PMID: 35995853] (ICC/IF, Human)
• Yoon Y, Go G, Yoon S Et Al. Melatonin Treatment Improves Renal Fibrosis via miR-4516/SIAH3/PINK1 Axis Cells 2021-07-03 [PMID: 34359852]
• Boukhedouni N, Martins C, Darrigade AS et al. Type-1 cytokines regulate MMP-9 production and E-cadherin disruption to promote melanocyte loss in vitiligo JCI Insight 2020-06-04 [PMID: 32369451] (IHC-P, Human)
• Koupenova M, Corkrey HA, Vitseva O et al. The role of platelets in mediating a response to human influenza infection. Nat Commun. 2018-09-01 [PMID: 30992428] (ICC/IF, Human)
  
  Details:
  Citation using the Azide Free form of this antibody.

Reviews for LAMP-1/CD107a Antibody (NBP2-52721) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 10/27/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: MDA MB 231 cells
• Species: Human
• Lot: A-1

reviewed by: Verified Customer ICC Human 06/28/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: MCF-7 human breast cancer cell line
• Species: Human
• Lot: a1

Comments

• Comments: Secondary Antibody Dy680

Bioinformatics

• Gene Symbol: LAMP1