LAMP-1/CD107a Antibody (107) - Azide and BSA Free

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Staining of human LAMP1 in human breast carcinoma with rabbit monoclonal antibody (1:1000).

Flow Cytometry: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Analysis of Human LAMP1(CD107a) on Jurkat cells. Cells were treated, stained with purified anti-Human LAMP1(CD107a), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Staining of human LAMP1 in human placenta with rabbit monoclonal antibody (1:1000). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

LAMP-1/CD107a was immunoprecipitated using NBP2-89844 at a 1:100 dilution and 60ug Immunomagnetic beads Protein A/G and Goat Anti-Rabbit IgG (H+L)[HRP] as a secondary antibody at 1/10000 dilution: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. Lane C: 0.5 mg Daudi Whole Cell Lysate. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 120 kDa

Western Blot: LAMP-1/CD107a Antibody (107) [NBP2-89844] - NBP2-89844 at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: LAMP-1/CD107a knockout Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 120 kDa. Observed band size: 100-130 kDa

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Analysis of Human LAMP1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89844 antibody (1:300). Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody, countstained with Alexa Fluor(R) 546-conjugated phallotoxins (red) and DAPI (blue). Positive staining was localized to lysosome membrane.

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IHC, IP
• Clone: 107
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

LAMP-1/CD107a Antibody (107) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Human LAMP-1/CD107a (Accession#: NP_005552.3; Met 1-Met 382).
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: LAMP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Flow Cytometry 1:20-1:100
  • Immunocytochemistry/ Immunofluorescence 1:20-1:100
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 0.2-1 uL/mg of lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for LAMP-1/CD107a Antibody (107) - Azide and BSA Free

• CD107 antigen-like family member A
• CD107a antigen
• CD107a
• LAMP-1
• LAMPA
• LGP120
• lysosomal-associated membrane protein 1
• lysosome-associated membrane glycoprotein 1
• Lysosome-associated membrane protein 1

Background

LAMP-1 (lysosome-associated membrane protein 1), also known as CD107a (cluster of differentiation 107a), is a major component of lysosomal membranes that plays an important role in lysosomal biogenesis, autophagy, and cholesterol metabolism (1). LAMP-1 is a type I transmembrane glycoprotein that is expressed on plasma membranes and the membranes of endosomes, autolysosomes, and lysosomes (1,2). Additionally, LAMP-1/CD107a is a commonly used marker for natural killer (NK) cell degranulation (3). LAMP-1 and another lysosomal-associated membrane protein, LAMP-2, together make up about half of all lysosome membrane proteins (1). Additionally, LAMP-1 has a role in presenting carbohydrate ligands to selectins (2). Human LAMP-1 protein is comprised of 417 amino acids (aa) with a theoretical molecular weight of 44.8 kDa; however, glycosylation can increase the molecular weight upwards of 120 kDa (1, 4). Structurally, LAMP-1 protein contains a large luminal/extracellular domain (29-382 aa), a helical transmembrane domain (383-405 aa), and a short cytoplasmic tail (406-417 aa) (1,2). Additionally, the protein has many N- and O-linked glycosylation sites which helps with stability in the membrane (1,2).

LAMP-1 plays an important role in autophagy-mediated ATP-release during apoptosis where lysosomes containing intracellular ATP migrate to the plasma membrane and, during exocytosis, LAMP-1 is exposed to the cell surface (5). Studies have found that knockdown of LAMP-1 blocks the ATP release from the cell (5). Furthermore, an absence of LAMP-1 and LAMP-2 leads to an accumulation of lysosomal cholesterol (6). Lysosomal membrane dysfunction or defects has also been associated with disease development (6,7). For example, one feature of pancreatitis is autophagy impairment which is caused by lysosomal dysfunction and a corresponding decrease in lysosomal-membrane associated proteins LAMP-1 and LAMP-2 (7).

References

1. Eskelinen E. L. (2006). Roles of LAMP-1 and LAMP-2 in lysosome biogenesis and autophagy. Molecular aspects of medicine, 27(5-6), 495-502. https://doi.org/10.1016/j.mam.2006.08.005

2. Cheng, X. T., Xie, Y. X., Zhou, B., Huang, N., Farfel-Becker, T., & Sheng, Z. H. (2018). Revisiting LAMP1 as a marker for degradative autophagy-lysosomal organelles in the nervous system. Autophagy, 14(8), 1472-1474. https://doi.org/10.1080/15548627.2018.1482147

3. Krzewski, K., & Coligan, J. E. (2012). Human NK cell lytic granules and regulation of their exocytosis. Frontiers in immunology, 3, 335. https://doi.org/10.3389/fimmu.2012.00335

4. Uniprot (P11279)

5. Wang, Y., Martins, I., Ma, Y., Kepp, O., Galluzzi, L., & Kroemer, G. (2013). Autophagy-dependent ATP release from dying cells via lysosomal exocytosis. Autophagy, 9(10), 1624-1625. https://doi.org/10.4161/auto.25873

6. Schwake, M., Schr0der, B., & Saftig, P. (2013). Lysosomal membrane proteins and their central role in physiology. Traffic (Copenhagen, Denmark), 14(7), 739-748. https://doi.org/10.1111/tra.12056

7. Gukovsky, I., Pandol, S. J., Mareninova, O. A., Shalbueva, N., Jia, W., & Gukovskaya, A. S. (2012). Impaired autophagy and organellar dysfunction in pancreatitis. Journal of gastroenterology and hepatology, 27 Suppl 2(Suppl 2), 27-32. https://doi.org/10.1111/j.1440-1746.2011.07004.x

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LAMP-1/CD107a Antibody (NBP2-89844)(1)

Publication using NBP2-89844

• Popescu NI, Cochran J, Duggan E et al. Internalization of Polymeric Bacterial Peptidoglycan Occurs through Either Actin or Dynamin Dependent Pathways Microorganisms Mar 3 2022 [PMID: 35336127] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: LAMP1