Laminin alpha 3/Laminin-5 Antibody (P3H9-2) [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB, IHC, IP, B/N, ICC/IF
• Clone: P3H9-2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

Laminin alpha 3/Laminin-5 Antibody (P3H9-2) [Alexa Fluor® 700] Summary

• Immunogen: Human keratinocytes
• Specificity: Detects human Laminin alpha 3/Laminin‑5 in Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Adhesion Blockade
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Laminin alpha 3/Laminin-5 Antibody (P3H9-2) [Alexa Fluor® 700]

• BM600 150kD subunit
• BM600
• E170
• Epiligrin 170 kDa subunit
• epiligrin alpha 3 subunit
• Epiligrin
• JEB2A
• JEB2B
• kalinin 165kD subunit
• Kalinin subunit alpha
• LAM3, alpha-3 subunit
• LAMA3
• laminin A3
• Laminin alpha 3
• laminin subunit alpha-3
• laminin-5 alpha 3 chain
• Laminin-5 subunit alpha
• Laminin5
• Laminin-5
• Laminin-6 subunit alpha
• Laminin-7 subunit alpha
• LAMNA
• LOCS
• nicein 150kD subunit
• Nicein subunit alpha

Background

Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with Integrins, Dystroglycan and other receptors, Laminins contribute to cell differentiation, cell shape and migration, and maintenance of tissue phenotypes and survival (1). Laminin-5, also known as Epiligrin, consists of an alpha 3, beta 3, and gamma 2 chain. It is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa and other epithelial glands and plays an important role in the initiation and maintenance of epithelial cell anchorage to the underlying connective tissue (2, 3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.