Laminin alpha 3/Laminin-5 Antibodies

HAF007). A specific band was detected for Laminin a3/Laminin-5 at approximately 190 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF007). A specific band was detected for Laminin a3/Laminin-5 at approximately 190 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human lung tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Laminin a3/ Laminin-5 Monoclonal Antibody (Catalog # MAB21441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Laminin a3/Laminin-5 at approximately 190 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

240-B) for 24 hours using Mouse Anti-Human Laminin a3/Laminin-5 Monoclonal Antibody (Catalog # MAB21441) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the perinuclear space. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> 240-B) for 24 hours using Mouse Anti-Human Laminin a3/Laminin-5 Monoclonal Antibody (Catalog # MAB21441) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the perinuclear space. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of epithelial cells in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of epithelial cells in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).View our protocol for Staining Intracellular Molecules." class="big_lightbox"> MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).View our protocol for Staining Intracellular Molecules." class="big_thumb" />

VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the basement membrane in frozen human Skin. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the basement membrane in frozen human Skin. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="Laminin  alpha 3/Laminin‑5 was detected in perfusion fixed frozen sections of human skin using Mouse Anti-Human Laminin  alpha 3/Laminin‑5 Monoclonal Antibody (Catalog # MAB11784) at 1.5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the basement membrane in frozen human Skin. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />