Laminin alpha 3/Laminin-5 Antibody (P3H9-2) [Unconjugated]

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Laminin alpha 3/Laminin-5 was detected in immersion fixed MCF 10A human breast epithelial cell line using 10 µg/mL Mouse Anti-Human Laminin alpha 3/Laminin-5 Monoclonal Antibody (Catalog # MAB2144) for 3 hours at ...read more
Laminin alpha 3/Laminin‑5 was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human Laminin alpha 3/Laminin‑5 Monoclonal Antibody (Catalog # MAB2144) at 25 µg/mL overnight at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP, B/N, ICC/IF
Clone
P3H9-2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Laminin alpha 3/Laminin-5 Antibody (P3H9-2) [Unconjugated] Summary

Immunogen
Human keratinocytes
Specificity
Detects human Laminin alpha 3/Laminin‑5 in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LAMA3
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation
  • Adhesion Blockade
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publication using
MAB2144 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Laminin alpha 3/Laminin-5 Antibody (P3H9-2) [Unconjugated]

  • Epiligrin
  • LAMA3
  • Laminin alpha 3
  • Laminin5
  • Laminin-5

Background

Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with Integrins, Dystroglycan and other receptors, Laminins contribute to cell differentiation, cell shape and migration, and maintenance of tissue phenotypes and survival (1). Laminin-5, also known as Epiligrin, consists of an alpha 3, beta 3, and gamma 2 chain. It is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa and other epithelial glands and plays an important role in the initiation and maintenance of epithelial cell anchorage to the underlying connective tissue (2, 3).

  1. Colognato, H. et al. (2000) Dev. Dyn. 218:213.
  2. Stahl, S. et al. (1997) J. Cell Sci. 110:55.
  3. Gonzales, M. et al. (1999) Mol. Biol. Cell 10:259.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Laminin alpha 3/Laminin-5 Antibody (MAB2144)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Neut.


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Video Protocols

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Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Laminin alpha 3/Laminin-5 Antibody (MAB2144)

Discover related pathways, diseases and genes to Laminin alpha 3/Laminin-5 Antibody (MAB2144). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Bioinformatics

Gene Symbol LAMA3