Lactate Dehydrogenase Activity Assay Kit (Colorimetric)

Lactate Dehydrogenase Activity Assay Kit (Colorimetric) [NBP3-24510] - Standard Reference Curve

Lactate Dehydrogenase Activity Assay Kit (Colorimetric) [NBP3-24510] - Assay Example

• Reactivity: All-NA
• Applications: Func
• Suitable Sample Type: Serum, plasma, hydrothorax, tissue and cell samples
• Standard Curve Range: 6 - 500 U/L
• Sensitivity: 6 U/L

Lactate Dehydrogenase Activity Assay Kit (Colorimetric) Summary

• Description: Detection instrument: Microplate reader (440 - 460 nm, optimum wavelength: 450 nm)
  Assay time: 45 min
• Standard Curve Range: 6 - 500 U/L
• Sensitivity: 6 U/L
• Inter-Assay: %CV < 2.4
• Intra-Assay: %CV < 1.8
• Spike Recovery: Average recovery rate: 98%
• Kit Type: Activity Assay Kit (Colorimetric)
• Gene: LDHB

Applications/Dilutions

• Dilutions:
  • Functional

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Lactate Dehydrogenase Activity Assay Kit (Colorimetric)

• EC 1.1.1
• EC 1.1.1.27
• lactate dehydrogenase B
• LDH heart subunit
• LDH-B
• LDH-H
• L-lactate dehydrogenase B chain
• Renal carcinoma antigen NY-REN-46
• TRG-5

Background

Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDHA, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36 kDa subunits. Every LDH molecule consists of four subunits, where each subunit is either H or M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H) -in the heart, LDH-2 (3H1M) -in the reticuloendothelial system, LDH-3 (2H2M) -in the lungs, LDH-4 (1H3M) -in the kidneys and LDH-5 (4M) -in the liver and striated muscle. Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a "flipped pattern") suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). In general, LDH is often used as a marker of tissue breakdown. LDH shows a cytoplasmic localization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: LDHB