Mouse Lactate Dehydrogenase B ELISA Kit (Colorimetric)

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Tissue homogenates and other biological fluids
• Standard Curve Range: 0.156 - 10 ng/mL
• Sensitivity: 0.064 ng/mL

Mouse Lactate Dehydrogenase B ELISA Kit (Colorimetric) Summary

• Description: Assay time: 3 hours
• Standard Curve Range: 0.156 - 10 ng/mL
• Sensitivity: 0.064 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12
• Intra-Assay: %CV < 10
• Spike Recovery: 80 - 98%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: LDHB

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Lactate Dehydrogenase B ELISA Kit (Colorimetric)

• EC 1.1.1
• EC 1.1.1.27
• Epididymis Secretory Protein Li 281
• HEL-S-281
• Lactate Dehydrogenase B
• LDH heart subunit
• LDHB
• LDH-B
• LDHBD
• LDH-H
• L-lactate dehydrogenase B chain
• Renal Carcinoma Antigen NY-REN-46
• TRG-5

Background

Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), because it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. All LDH subunits can combine to form various terameric isoenzymes (140 kDa). Lactate dehydrogenase B (LDH-B, heart subunit, LDH-H) is involved in the conversion of L-lactate and NAD to pryruvate and NADH and it is predominantly localized in the heart tissue. Similar to other LDH subunit, LDH-B is considered to be an important marker for germ cell tumor (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: LDHB