Kremen-2 Antibody - BSA Free

Western Blot: Kremen-2 Antibody [NBP2-22339] - Western Blot Validation in Mouse Spleen Tissue Lysate. Loading: 15 ug of lysates per lane. Antibodies: KREMEN2 NBP2-22339 (Lane 1: 1 ug/mL and Lane 2: 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/Immunofluorescence: Kremen-2 Antibody [NBP2-22339] - KREMEN2 in HeLa cells with KREMEN2 antibody at 5 ug/ml.

Western Blot: Kremen-2 Antibody [NBP2-22339] - Independent Antibody Validation via Protein Expression Profile in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Kremen-2 [NBP2-22339] (2 ug/ml), Kremen-2 antibody from Company B (1 ug/ml) and beta-actin [NBP1-76692] (2 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: Kremen-2 Antibody [NBP2-22339] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: KREMEN2, NBP2-22339 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Kremen-2 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human Kremen-2. The immunogen is located within amino acids 390-440 of Kremen-2. Amino Acid Sequence: ALGASRGGPRRSWAV
• Specificity: Multiple isoforms of Kremen-2 are known to exist. Kremen-2 antibody is predicted to not cross-react with other Kremen protein family members. Human Kremen-2 has 6 isoforms, including isoform 1 (462aa, 49kD), isoform 2 (424aa, 45kD), isoform 3 (420aa, 44kD), isoform 4 (399aa, 42kD), isoform 5 (423aa, 45kD), and isoform 6 (381aa, 40kD). Mouse Kremen-2 has one isoform (461aa, 49kD) and rat Kremen-2 also has one isoform (468aa, 50kD). NBP2-22339 can detect human, mouse and rat.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KREMEN2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 5 ug/mL
  • Western Blot 1-2 ug/mL
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Kremen-2 Antibody - BSA Free

• Dickkopf receptor 2
• kremen protein 2
• Kremen2
• Kremen-2
• kringle containing transmembrane protein 2
• Kringle domain-containing transmembrane protein 2
• KRM2Kringle-containing protein marking the eye and the nose
• MGC10791
• MGC16709

Background

KREMEN2 encodes a high-affinity dickkopf homolog 1 (DKK1) transmembrane receptor that functionally cooperates with DKK1 to block wingless (WNT)/beta-catenin signaling. The encoded protein forms a ternary membrane complex with DKK1 and the WNT receptor lipoprotein receptor-related protein 6 (LRP6), and induces rapid endocytosis and removal of LRP6 from the plasma membrane. It contains extracellular kringle, WSC, and CUB domains. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KREMEN2
• Uniprot:
  • NP_757384(Human)