Human Dkk-2 ELISA Kit (Colorimetric)

ELISA: Human Dkk-2 ELISA Kit (Colorimetric) [NBP2-82468] - Samples were spiked with high concentrations of Human Dkk-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Dkk-2 ELISA Kit (Colorimetric) [NBP2-82468] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL

Human Dkk-2 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human Dkk-2 in samples. No Significant cross-reactivity or interference between Human Dkk-2 and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.89
• Intra-Assay: CV% < 4.82
• Spike Recovery: 89-110%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: DKK2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Dkk-2 ELISA Kit (Colorimetric)

• dickkopf (Xenopus laevis) homolog 2
• dickkopf 2
• dickkopf homolog 2 (Xenopus laevis)
• dickkopf related protein-2
• dickkopf-2
• dickkopf-related protein 2
• Dkk2
• Dkk-2
• hDkk-2

Background

Analysis of individual DKK domains and chimeric DKKs shows that the carboxy-terminal domains of both DKK1 and DKK2 associate with LRP6 and are necessary and sufficient for Wnt8 inhibition. Xenopus Dickkopf 1 (DKK1) was initially discovered as a Wnt antagonist that plays an important role in head formation. By far, four members of DKK have been identified in mammals. Each DKK molecule contains two conserved cysteine-rich domains. Recent studies showed that the second Cys-rich domains of DKK1 and DKK2 inhibited Wnt3a-activated signaling, whereas the first Cys-rich domains had no effects. In addition, the second Cys-rich domain of DKK2, but not that of DKK1, was able to activate the canonical pathway in the presence of LRP6, and this LRP-dependent signaling does not require Dvl.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Dkk-2 ELISA Kit (NBP2-82468). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: DKK2