Recombinant Human MSP/MST1 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human MSP/MST1 Protein Summary

Details of Functionality
Measured by its ability to activate MSP R/Ron in MDA-MB-453 or T47D human breast cancer cells. 80 ng/mL of the rhMSP significantly induces phosphorylation of MSP R/Ron measured by DuoSet IC human phospho-MSP R/Ron kit (Catalog # DYC1947).
Source
Mouse myeloma cell line, NS0-derived human MSP/MST1 protein
Gln19-Gly711
Accession #
N-terminal Sequence
No results obtained, Gln19 predicted & Val484
Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
Gene
MST1
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
78.5 kDa (MSP alpha / beta ); 53.3 kDa ( alpha chain), 25.2 kDa ( beta chain).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
81 kDa, 62 kDa and 30 kDa, reducing conditions
Publications
Read Publications using
352-MS in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MSP/MST1 Protein

  • D3F15S2
  • EC 3.4.21
  • hepatocyte growth factor-like protein homolog
  • HGFL
  • HGFLhepatocyte growth factor-like protein
  • macrophage stimulating 1 (hepatocyte growth factor-like)
  • Macrophage stimulatory protein
  • Macrophage-stimulating protein
  • MSP
  • MSPDNF15S2
  • MST1
  • NF15S2
  • SF2

Background

Macrophage stimulating protein (MSP), also known as HGF-like protein, and scatter factor-2, is a member of the HGF family of growth factors (1). MSP is secreted as an inactive single chain precursor (pro-MSP) that contains a PAN/APPLE-like domain, four kringle domains, and a peptidase S1 domain which lacks enzymatic activity (2). Human MSP shares 79% aa sequence identity with mouse MSP and 44% aa sequence identity with human HGF. Pro-MSP is secreted by hepatocytes under the positive and negative control of CBP in complex with either HNF-4 or RAR, respectively (3). Circulating pro-MSP is proteolytically cleaved in response to tissue injury to yield biologically active disulfide linked heterodimers consisting of a 45 - 62 kDa alpha and a 25-35 kDa beta chain (4, 5). Pro-MSP can be activated by MT-SP1, a transmembrane protease that is expressed on macrophages and is upregulated in many cancers (6). Heterodimeric MSP as well as the isolated beta chain bind to MSP R/Ron with high-affinity, although only heterodimeric MSP can induce receptor dimerization and signaling (7, 8). MSP induces macrophage and keratinocyte proliferation and osteoclast activation (9, 10). It also inhibits LPS- or IFN-induced iNOS and IL-12 expression by macrophages and prevents apoptosis of epithelial cells separated from the ECM (11, 12).

  1. Wang, M.-H. et al. (2002) Scand. J. Immunol. 56:545.
  2. Han, S. et al. (1991) Biochemistry 30:9768.
  3. Muraoka, R.S. et al. (1999) Endocrinology 140:187.
  4. Wang, M.-H. et al. (1996) J. Clin. Invest. 97:720.
  5. Nanney, L.B. et al. (1998) J. Invest. Dermatol. 111:573.
  6. Bhatt, A.S. et al. (2007) Proc. Natl. Acad. Sci. 104:5771.
  7. Wang, M.-H. et al. (1997) J. Biol. Chem. 272:16999.
  8. Danilkovitch, A. et al. (1999) J. Biol. Chem. 274:29937.
  9. Wang, M.-H. et al. (1996) Exp. Cell Res. 226:39.
  10. Kurihara, N. et al. (1998) Exp. Hematol. 26:1080.
  11. Morrison, A.C. et al. (2004) J. Immunol. 172:1825.
  12. Liu, Q.P. et al. (1999) J. Immunol. 163:6606.

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352-MS
Species: Hu
Applications: Bioactivity

Publications for MSP/MST1 (352-MS)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Bioassay, Enzyme Substrate.


Filter By Application
Bioassay
(7)
Enzyme Substrate
(1)
All Applications
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Human
(7)
Mouse
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using 352-MS Applications Species
A Khaibullin, EA Adjei, N Afangbedji, A Ivanov, N Kumari, LEF Almeida, ZMN Quezado, S Nekhai, M Jerebtsova RON kinase inhibition reduces renal endothelial injury in sickle cell disease mice Haematologica, 2018;0(0):. 2018 [PMID: 29519868] (Bioassay, Mouse) Bioassay Mouse
B Yin, Z Liu, Y Wang, X Wang, W Liu, P Yu, X Duan, C Liu, Y Chen, Y Zhang, X Pan, H Yao, Z Liao, Z Tao RON and c-Met facilitate metastasis through the ERK signaling pathway in prostate cancer cells Oncol. Rep., 2017;0(0):. 2017 [PMID: 28440432] (Bioassay, Human) Bioassay Human
GI Vladimer, B Snijder, N Krall, JW Bigenzahn, KVM Huber, CH Lardeau, K Sanjiv, A Ringler, UW Berglund, M Sabler, OL de la Fuen, P Knöbl, S Kubicek, T Helleday, U Jäger, G Superti-Fu Global survey of the immunomodulatory potential of common drugs Nat. Chem. Biol., 2017;0(0):. 2017 [PMID: 28437395] (Bioassay, Human) Bioassay Human
Zhou D, Li C, Yang L Variant RONDelta160 of the RON receptor tyrosine kinase promotes the growth and invasion in vitro and in vivo in gastric cancer cell lines. Cancer Cell Int, 2015;15(1):9. 2015 [PMID: 25685065] (Bioassay, Human) Bioassay Human
Yang C, Kim J, Kim T, Lee P, Kim S, Lee H, Shin D, Nguyen L, Lee M, Jin H, Kim K, Lee C, Kim M, Park S, Kim J, Choi H, Jo E Small heterodimer partner interacts with NLRP3 and negatively regulates activation of the NLRP3 inflammasome. Nat Commun, 2015;6(0):6115. 2015 [PMID: 25655831] (Bioassay, Human) Bioassay Human
Bhatt AS, Welm A, Farady CJ, Vasquez M, Wilson K, Craik CS Coordinate expression and functional profiling identify an extracellular proteolytic signaling pathway. Proc. Natl. Acad. Sci. U.S.A., 2007;104(14):5771-6. 2007 [PMID: 17389401] (Enzyme Substrate, Human) Enzyme Substrate Human
Gunella G, Bardelli C, Amoruso A Macrophage-stimulating protein differently affects human alveolar macrophages from smoker and non-smoker patients: evaluation of respiratory burst, cytokine release and NF-kappaB pathway. Br. J. Pharmacol., 2006;148(4):478-89. 2006 [PMID: 16633352] (Bioassay, Human) Bioassay Human
Rampino T, Collesi C, Gregorini M Macrophage-stimulating protein is produced by tubular cells and activates mesangial cells. J. Am. Soc. Nephrol., 2002;13(3):649-57. 2002 [PMID: 11856768] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol MST1
Uniprot