Dkk-3 Antibody [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Dkk-3 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Dkk-3
Ala22-Ile350
Accession # Q9UBP4
Specificity
Detects human Dkk-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant mouse Dkk-3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DKK3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF1118 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dkk-3 Antibody [Unconjugated]

  • dickkopf (Xenopus laevis) homolog 3
  • dickkopf 3
  • dickkopf homolog 3 (Xenopus laevis)
  • Dickkopf-3
  • dickkopf-related protein 3
  • Dkk3
  • Dkk-3
  • hDkk-3
  • regulated in glioma
  • REIC
  • REICdkk-3
  • RIG
  • RIG-like 5-6
  • RIG-like 7-1

Background

Dkk-3, also known as REIC (Reduced Expansion in Immortalized Cells), is one of four numbered members of the Dickkopf family of Wnt antagonists (1). Dkk-3 is a secreted monomer expressed in many normal human tissues, most strongly in heart, brain and spinal cord (1, 2), and during early embryonic development in the mouse (3). N-glycosylation at up to four sites preceding or between two conserved cysteine-rich motifs results in expression of a 38 kDa glycoprotein (1, 4). The cysteine-rich motifs contain 10 cysteines each, with prokineticin and colipase families containing sequences similar to those of the second motif (1, 5). Human Dkk-3 shows 82%, 88%, 85%, and 53% amino acid (aa) identity with mouse, bovine, canine, and chick Dkk-3, respectively, and 37-45% aa identity with other human Dkk family members. Several lines of evidence implicate Dkk-3 as a negative growth regulator. Dkk-3 is downregulated in many tumors as compared to normal cells, sometimes by loss of heterozygosity (4, 6). Downregulation by CpG hypermethylation in acute lymphoblastic leukemia is correlated with faster progression and shorter survival (7). Release of cultured cells from serum starvation results in downregulation of Dkk-3 in late G1 phase of the cell cycle (6). Over-expression of
Dkk‑3 results in tumor cell-line-specific growth inhibition, induction of apoptosis, and decreased tumorigenicity in nude mice (2, 4, 6). The prototype Dickkopf member, Dkk-1, antagonizes Wnt family signaling by binding to Wnt receptors LRP5 and LRP6 (low-density lipoprotein receptor-related proteins) and promoting their internalization (1, 9, 10). Results are less straightforward for Dkk-3, where some studies show binding to LRP5/6 while others do not. These effects appear to be dependent on the cells and conditions used (1, 6-10).

  1. Krupnik, V.E. et al. (1999) Gene 238:301.
  2. Tsuji, T. et al. (2000) Biochem. Biophys. Res. Comm. 268:20.
  3. Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
  4. Hsieh, S.-Y. et al. (2004) Oncogene 23:9183.
  5. Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582.
  6. Tsuji, T. et al. (2001) Biochem. Biophys. Res. Comm. 289:257.
  7. Roman-Gomez, J. et al. (2004) Br. J. Cancer 91:707.
  8. Hoang, B.H. et al. (2004) Cancer Res. 64:2734.
  9. Caricasole, A. et al. (2003) J. Biol. Chem. 278:37024.
  10. Mao, B. et al. (2001) Nature 411:321.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dkk-3 Antibody (AF1118)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC, IHC-P, Western Blot.


Filter By Application
ICC
(2)
IHC-P
(3)
Western Blot
(4)
All Applications
Filter By Species
Human
(9)
All Species
Showing Publications 1 - 9 of 9.
Publications using AF1118 Applications Species
N Chiyonobu, S Shimada, Y Akiyama, K Mogushi, M Itoh, K Akahoshi, S Matsumura, K Ogawa, H Ono, Y Mitsunori, D Ban, A Kudo, S Arii, T Suganami, S Yamaoka, Y Ogawa, M Tanabe, S Tanaka Fatty acid binding protein 4 (FABP4) overexpression in intratumoral hepatic stellate cells within hepatocellular carcinoma with metabolic risk factors Am. J. Pathol., 2018;0(0):. 2018 [PMID: 29454748] (IHC-P, Human) IHC-P Human
Loss of Dickkopf 3 Promotes the Tumorigenesis of Basal Breast Cancer PLoS ONE, 2016;11(7):e0160077. 2016 [PMID: 27467270] (Western Blot, Human) Western Blot Human
Riordan D, Varma S, West R, Brown P Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling. PLoS ONE, 2015;10(7):e0128975. 2015 [PMID: 26176839] (IHC-P, Human) IHC-P Human
Xiang T, Li L, Yin X, Zhong L, Peng W, Qiu Z, Ren G, Tao Q Epigenetic silencing of the WNT antagonist Dickkopf 3 disrupts normal Wnt/beta-catenin signalling and apoptosis regulation in breast cancer cells. J Cell Mol Med, 2013;17(10):1236-46. 2013 [PMID: 23890219] (Western Blot, Human) Western Blot Human
Wang W, Zhu W, Xu XY The clinicopathological significance of REIC expression in colorectal carcinomas. Histol. Histopathol., 2012;27(6):735-43. 2012 [PMID: 22473694] (Western Blot, Human) Western Blot Human
Zhang K, Watanabe M, Kashiwakura Y, Li SA, Edamura K, Huang P, Yamaguchi K, Nasu Y, Kobayashi Y, Sakaguchi M, Ochiai K, Yamada H, Takei K, Ueki H, Huh NH, Li M, Kaku H, Na Y, Kumon H Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development. Int. J. Oncol., 2010;37(6):1495-501. 2010 [PMID: 21042718] (ICC, Human) ICC Human
Choi W, Wolber R, Gerwat W The fibroblast-derived paracrine factor neuregulin-1 has a novel role in regulating the constitutive color and melanocyte function in human skin. J. Cell. Sci., 2010;123(0):3102-11. 2010 [PMID: 20736300] (Western Blot, Human) Western Blot Human
Choi W, Miyamura Y, Wolber R, Smuda C, Reinhold W, Liu H, Kolbe L, Hearing VJ Regulation of human skin pigmentation in situ by repetitive UV exposure: molecular characterization of responses to UVA and/or UVB. J. Invest. Dermatol., 2010;130(6):1685-96. 2010 [PMID: 20147966] (IHC-P, Human) IHC-P Human
Untergasser G, Steurer M, Zimmermann M, Hermann M, Kern J, Amberger A, Gastl G, Gunsilius E The Dickkopf-homolog 3 is expressed in tumor endothelial cells and supports capillary formation. Int. J. Cancer, 2008;122(7):1539-47. 2008 [PMID: 18033687] (ICC, Human) ICC Human

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Biotin BAF1118

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Bioinformatics

Gene Symbol DKK3
Uniprot