Kremen-1 Antibody

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Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Kremen‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1647) followed by HRP-conjugated ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Kremen-1 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Kremen‑1
Ala20-Gly395
Accession # Q640Q6
Specificity
Detects mouse Kremen-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human Kremen-2 and recombinant mouse Kremen-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KREMEN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
Publications
Read Publications using
AF1647 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kremen-1 Antibody

  • EC 3.4.21
  • FLJ31863
  • KREMEM1
  • kremen protein 1
  • KREMEN
  • Kremen1
  • Kremen-1
  • kringle containing transmembrane protein 1
  • kringle containing transmembrane protein
  • Kringle domain-containing transmembrane protein 1
  • kringle-coding gene marking the eye and the nose
  • Kringle-containing protein marking the eye and the nose
  • KRM1
  • KRM1Dickkopf receptor

Background

Kremen (Kringle-containing protein marking the eye and the nose) proteins are type I transmembrane proteins that contain extracellular kringle, WSC and CUB domains and an intracellular region without any conserved motifs (1). Two related members, Kremen-1 and -2, have been identified. Kremens bind a subset of the secreted Dickkopf (Dkk) proteins (Dkk-1, -2, and -4) with high affinity to modulate the canonical Wnt signaling pathway that is transduced by the ternary receptor complex composed of Wnt, the seven-transmembrane domain receptor Frizzled, and the LDL-receptor-related protein 5/6 (LRP5/6) co-receptor (2, 3). Within the Dkk family, Dkk-1 and -4 bind directly to the LRP5/6 co-receptor to antagonize the canonical Wnt/ beta -catenin signaling pathway, but not the planar cell polarity (PCP) signaling pathway that does not involve LRP5/6 (4). In contrast, Dkk-3 has no effect on Wnt signaling and Dkk-2 can function either as an LRP agonist or antagonist, depending on whether the cell expresses Kremen (5). Kremen co-operates with Dkk to antagonize Wnt signaling via formation of a Kremen-Dkk-LRP ternary complex that triggers the internalization and clearance of the complex from the cell surface (3). All three extracellular domains but not the cytoplasmic region of a membrane anchored Kremen are needed for binding to the second cysteine-rich domain of Dkks (3). Mouse Kremen-1 cDNA encodes a 473 amino acid (aa) glycosylated protein with a putative 19 aa signal peptide, a 372 aa extracellular domain, a 21 aa transmembrane domain and a 60 aa cytoplasmic domain. In the extracellular domain, it shares 92% and 41% amino acid sequence identity with human Kremen-1 and mouse Kremen-2, respectively. Mouse Kremen-1 is widely expressed in diverse embryonic (apical ectodermal ridge of the developing fore- and hindlimb buds, telencephalon and the first brachial arch, myotome and sensory tissues) and adult (lung, heart, kidney, skeletal muscle and testis) tissues (1).

  1. Nakamura, T. et al. (2001) Biochim Biophys Acta 1518:63.
  2. Davidson G. et al. (2002) Development 129:5587.
  3. Mao, B. et al. (2002) Nature 417:664.
  4. Zorn, A.M. (2001) Curr. Biol. 11:R592.
  5. Mao, B. and C. Niehrs (2003) Gene 302:179.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Kremen-1 Antibody (AF1647)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC, Western Blot.


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Biotin BAF1647

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Bioinformatics

Gene Symbol KREMEN1
Uniprot