KMT2D Antibody

Immunocytochemistry/Immunofluorescence: KMT2D Antibody [NBP1-89123] - Staining of human cell line A-431 shows localization to nucleus, plasma membrane & cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: KMT2D Antibody [NBP1-89123] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: KMT2D Antibody [NBP1-89123] - Staining of human cervix, uterine shows weak nuclear positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: KMT2D Antibody [NBP1-89123] - Staining of human rectum shows moderate nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: KMT2D Antibody [NBP1-89123] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

KMT2D Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: AQLADTLFSKGLGPWDPPDNLAETQKPEQSSLVPGHLDQVNGQVVPEASQLSIKQEPREEPCALGAQSVKREANGEPIGAPGTSNHLLLAGPRSEAGHLLLQKLLRAKNVQLSTGRGSEGLRAEINGHI
• Predicted Species: Mouse (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MLL2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot
• Application Notes: Use in WB reported in scientific literature (PMID:34077726). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization, Use PFA/Triton X-100.
• Control Peptide:

  KMT2D Protein (NBP1-89123PEP)

Reactivity Notes

Use in Human reported in scientific literature (PMID:33801954).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for KMT2D Antibody

• AAD10
• ALRALL1-related protein
• CAGL114
• EC 2.1.1.43
• histone-lysine N-methyltransferase MLL2
• KMT2D
• Lysine N-methyltransferase 2B
• MLL4
• myeloid/lymphoid or mixed-lineage leukemia 2
• Myeloid/lymphoid or mixed-lineage leukemia protein 2
• TNRC21
• trinucleotide repeat containing 21

Background

Histone methyltransferase. Methylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2. Acts as a coactivator for estrogen receptor by being recruited by ESR1, thereby activating transcription

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KMT2D Antibody (NBP1-89123)(4)

Publications using NBP1-89123

• Puvvula PK, Yu Y, Sullivan KR et al. Inhibiting an RBM39/MLL1 epigenomic regulatory complex with dominant-negative peptides disrupts cancer cell transcription and proliferation Cell reports 2021-06-01 [PMID: 34077726] (WB, Human)
• Kim J, Park J, Shin S et al. Neo-Fs Index: A Novel Immunohistochemical Biomarker Panel Predicts Survival and Response to Anti-Angiogenetic Agents in Clear Cell Renal Cell Carcinoma Cancers 2021-03-10 [PMID: 33801954] (IHC, Human)
• Jiyuan Zhang, David Dominguez-Sola, Shafinaz Hussein et al. Disruption of KMT2D perturbs germinal center B cell development and promotes lymphomagenesis. Nature Medicine 2015-09-14 [PMID: 26366712] (ICC/IF, IHC, Human)
• Dubuc AM, Remke M, Korshunov A et al. Aberrant patterns of H3K4 and H3K27 histone lysine methylation occur across subgroups in medulloblastoma. Acta Neuropathol 2013-03-01 [PMID: 23184418]

Bioinformatics

• Gene Symbol: MLL2