ASH2L Antibody

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Western Blot: ASH2L Antibody [NB600-250] - Detection of human ASH2 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Immunohistochemistry: ASH2L Antibody [NB600-250] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ASH2 used at a dilution of 1:1,000 ( 1ug/ml). Detection: DAB staining using ...read more
Chromatin Immunoprecipitation: ASH2L Antibody [NB600-250] - A. 10 ug of was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference ...read more
Western Blot: ASH2L Antibody [NB600-250] - Detection of Human ASH2 on HeLa whole cell lysates using NB600-250. Immunoprecipitated ASH2 was detected by WB using affinity purified rabbit anti-ASH2 antibody NB600-251 used.
Independent Antibodies: Western Blot: ASH2L Antibody [NB600-250] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: ...read more
Immunohistochemistry-Paraffin: ASH2L Antibody [NB600-250] - Human laryngeal squamous cell carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P, IP, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

ASH2L Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human Absent, Small, or Homeotic-Like 2 using the numbering given in entry NP_004665.1 (GeneID 9070).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ASH2L
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Chromatin Immunoprecipitation 10ug
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Chromatin Immunoprecipitation (ChIP)
Application Notes
This antibody is useful for: Western Blot: 1:2,000 to 1:20,000 Immunoprecipitation: 2 to 5 mcg/mg lysate Chromatin IP (ChIP): 1 to 5 mcg
Control
ASH2L Overexpression Lysate
Publications
Read Publications using NB600-250.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ASH2L Antibody

  • ash2 (absent, small, or homeotic)-like (Drosophila)
  • ASH2
  • ASH2L1ash2 (absent, small, or homeotic, Drosophila, homolog)-like
  • ASH2L2ASH2-like protein
  • Bre2
  • set1/Ash2 histone methyltransferase complex subunit ASH2

Background

The ash2 gene is a member of the trithorax group of genes whose products function to maintain active transcription of homeotic selector genes. Mutations in ash2 cause the homeotic transformations expected for a gene in this group but, in addition, cause a variety of pattern formation defects that are not necessarily expected. The ash2 gene is located in cytogenetic region 96A17-19 flanked by slowpoke and tolloid and is included in a cosmid that contains part of slowpoke. Ash2, the product of the Drosophila trxG gene absent, small, or homeotic discs 2 (ash2) is a component of a 500-kD complex. ASH2 binds directly to Skittles (Sktl), a predicted phosphatidylinositol 4-phosphate 5-kinase, and the association of these proteins is functionally significant. Histone H1 hyperphosphorylation is dramatically increased in both ash2 and sktl mutant polytene chromosomes. These results suggest that Ash2 maintains active transcription by binding a producer of nuclear phosphoinositides and downregulating histone H1 hyperphosphorylation. Both Ash2 and Sktl accumulate on polytene chromosomes and in the nucleolus. These results suggest that their physical association and functions involve chromatin and, perhaps, ribosomal DNA transcription. Ash2 accumulates normally on sktl mutant chromosomes while Sktl does not accumulate on ash2 mutant chromosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ASH2L Antibody (NB600-250)(12)


Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB600-250 Applications Species
Choi NM, Boss JM. Multiple histone methyl and acetyltransferase complex components bind the HLA-DRA gene. PLoS One 2012 [PMID: 22701520]
Dharmarajan V, Lee JH, Patel A et al. Structural basis for WDR5 interaction (Win) motif recognition in human SET1 family histone methyltransferases. J Biol Chem 2012 Aug [PMID: 22665483]
Schwab KR, Patel SR, Dressler GR et al. Role of PTIP in class switch recombination and long-range chromatin interactions at the immunoglobulin heavy chain locus. Mol Cell Biol 2011 Apr [PMID: 21282469]
Amat R, Gudas LJ. RARgammais required for correct deposition and removal of Suz12 and H2A.Z in embryonic stem cells. J Cell Physiol 2011 Feb [PMID: 20857416]
Pullirsch D, Hartel R, Kishimoto H et al. The Trithorax group protein Ash2l and Saf-A are recruited to the inactive X chromosome at the onset of stable X inactivation. Development 2010 Mar [PMID: 20150277]
Tate CM, Lee JH, Skalnik DG et al. CXXC finger protein 1 contains redundant functional domains that support embryonic stem cell cytosine methylation, histone methylation, and differentiation. Mol Cell Biol 2009 Jul [PMID: 19433449]
Lee J, Saha PK, Yang QH et al. Targeted inactivation of MLL3 histone H3-Lys-4 methyltransferase activity in the mouse reveals vital roles for MLL3 in adipogenesis. Proc Natl Acad Sci U S A 2008 Dec [PMID: 19047629]
Nguyen P, Bar-Sela G, Sun L et al. BAT3 and SET1A form a complex with CTCFL/BORIS to modulate H3K4 histone dimethylation and gene expression. Mol Cell Biol 2008 Nov [PMID: 18765639]
Guertin DA, Guntur KV, Bell GW et al. Functional genomics identifies TOR-regulated genes that control growth and division. Curr Biol 2006 May [PMID: 16713952]
Mo R, Rao SM, Zhu YJ et al. Identification of the MLL2 complex as a coactivator for estrogen receptor alpha. J Biol Chem 2006 Jun [PMID: 16603732]
Show All 12 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ASH2L
Entrez
Uniprot