ASH2L Antibody


Western Blot: ASH2L Antibody [NB600-250] - Detection of human ASH2 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit more
Immunohistochemistry-Paraffin: ASH2L Antibody [NB600-250] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ASH2used at a dilution of 1:1,000 ( 1ug/ml).Detection: DAB
Independent Antibodies: Western Blot: ASH2L Antibody [NB600-250] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: more

Product Details

Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
1.0 mg/ml
Validated by:

Independent Antibodies


Order Details

ASH2L Antibody Summary

The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human Absent, Small, or Homeotic-Like 2 using the numbering given in entry NP_004665.1 (GeneID 9070).
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
ASH2L Overexpression Lysate
Read Publications using
NB600-250 in the following applications:

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for ASH2L Antibody

  • ash2 (absent, small, or homeotic)-like (Drosophila)
  • ASH2
  • ASH2L1ash2 (absent, small, or homeotic, Drosophila, homolog)-like
  • ASH2L2ASH2-like protein
  • Bre2
  • set1/Ash2 histone methyltransferase complex subunit ASH2


The ash2 gene is a member of the trithorax group of genes whose products function to maintain active transcription of homeotic selector genes. Mutations in ash2 cause the homeotic transformations expected for a gene in this group but, in addition, cause a variety of pattern formation defects that are not necessarily expected. The ash2 gene is located in cytogenetic region 96A17-19 flanked by slowpoke and tolloid and is included in a cosmid that contains part of slowpoke. Ash2, the product of the Drosophila trxG gene absent, small, or homeotic discs 2 (ash2) is a component of a 500-kD complex. ASH2 binds directly to Skittles (Sktl), a predicted phosphatidylinositol 4-phosphate 5-kinase, and the association of these proteins is functionally significant. Histone H1 hyperphosphorylation is dramatically increased in both ash2 and sktl mutant polytene chromosomes. These results suggest that Ash2 maintains active transcription by binding a producer of nuclear phosphoinositides and downregulating histone H1 hyperphosphorylation. Both Ash2 and Sktl accumulate on polytene chromosomes and in the nucleolus. These results suggest that their physical association and functions involve chromatin and, perhaps, ribosomal DNA transcription. Ash2 accumulates normally on sktl mutant chromosomes while Sktl does not accumulate on ash2 mutant chromosomes.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ASH2L Antibody (NB600-250)(13)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC, IHC-P.

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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB600-250 Applications Species
Choi NM, Boss JM. Multiple histone methyl and acetyltransferase complex components bind the HLA-DRA gene. PLoS One 2012-01-01 [PMID: 22701520]
Dharmarajan V, Lee JH, Patel A et al. Structural basis for WDR5 interaction (Win) motif recognition in human SET1 family histone methyltransferases. J Biol Chem 2012-08-01 [PMID: 22665483]
Schwab KR, Patel SR, Dressler GR et al. Role of PTIP in class switch recombination and long-range chromatin interactions at the immunoglobulin heavy chain locus. Mol Cell Biol 2011-04-01 [PMID: 21282469]
Amat R, Gudas LJ. RARgammais required for correct deposition and removal of Suz12 and H2A.Z in embryonic stem cells. J Cell Physiol 2011-02-01 [PMID: 20857416]
Pullirsch D, Hartel R, Kishimoto H et al. The Trithorax group protein Ash2l and Saf-A are recruited to the inactive X chromosome at the onset of stable X inactivation. Development 2010-03-01 [PMID: 20150277]
Tate CM, Lee JH, Skalnik DG et al. CXXC finger protein 1 contains redundant functional domains that support embryonic stem cell cytosine methylation, histone methylation, and differentiation. Mol Cell Biol 2009-07-01 [PMID: 19433449]
Lee J, Saha PK, Yang QH et al. Targeted inactivation of MLL3 histone H3-Lys-4 methyltransferase activity in the mouse reveals vital roles for MLL3 in adipogenesis. Proc Natl Acad Sci U S A 2008-12-01 [PMID: 19047629]
Nguyen P, Bar-Sela G, Sun L et al. BAT3 and SET1A form a complex with CTCFL/BORIS to modulate H3K4 histone dimethylation and gene expression. Mol Cell Biol 2008-11-01 [PMID: 18765639]
Guertin DA, Guntur KV, Bell GW et al. Functional genomics identifies TOR-regulated genes that control growth and division. Curr Biol 2006-05-01 [PMID: 16713952]
Mo R, Rao SM, Zhu YJ et al. Identification of the MLL2 complex as a coactivator for estrogen receptor alpha. J Biol Chem 2006-06-01 [PMID: 16603732]
Show All 13 Publications.

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Gene Symbol ASH2L