SETD1A Antibody

Images

 
Western Blot: SETD1A Antibody [NB100-558] - Detection of human hSET1 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HeLa, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity ...read more
Immunohistochemistry-Paraffin: SETD1A Antibody [NB100-558] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-hSET1 used at 1:5,000 (0.2 ug/ml). Secondary: HRP-conjugated goat anti-rabbit ...read more
Chromatin Immunoprecipitation: SETD1A Antibody [NB100-558] - Chromatin Immunoprecipitation: SETD1A Antibody [NB100-558] - ChIP-chip scatter plot of anti-hSET1 (NB100-558) enriched DNA binding sites versus input ...read more
Independent Antibodies: Immunoprecipitation: SETD1A Antibody [NB100-558] - Detection of human hSET1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, IHC, IHC-P, IP, CHIP-SEQ
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

SETD1A Antibody Summary

Immunogen
A synthetic peptide which maps to a region between residues 1200 and 1250 of human hSET1 (KIAA0339) using the numbering given in TrEMBL entry O15047 (GeneID 9739).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SETD1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000 - 1:25000
  • Chromatin Immunoprecipitation 10ug
  • Immunohistochemistry 1:2000 - 1:10000
  • Immunohistochemistry-Paraffin 1:2000 - 1:10000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Chromatin Immunoprecipitation Sequencing
Publications
Read Publications using
NB100-558 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SETD1A Antibody

  • histone-lysine N-methyltransferase SETD1A
  • hSET1A
  • KIAA0339EC 2.1.1.43
  • KMT2FSET1
  • Lysine N-methyltransferase 2F
  • SET domain containing 1A
  • SET domain-containing protein 1A
  • Set1
  • Set1/Ash2 histone methyltransferase complex subunit SET1
  • SET1A

Background

SET1A is a component of a histone methyltransferase (HMT) complex that produces mono-, di-, and trimethylated histone H3 at Lys4. The complex is the analog of the S. cerevisiae Set1/COMPASS complex (Lee and Skalnik, 2005 [PubMed 16253997]). Also see SET1B (MIM 611055).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SETD1A Antibody (NB100-558)(5)

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Product General Protocols

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Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for SETD1A Antibody (NB100-558) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SETD1A
Entrez
Uniprot