SETD1A Antibody

Western Blot: SETD1A Antibody [NB100-558] - Detection of human hSET1 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HeLa, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-hSET1 antibody A300-289A (lot A300-289A-6) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Immunohistochemistry-Paraffin: SETD1A Antibody [NB100-558] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-hSET1 used at 1:5,000 (0.2 ug/ml). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Chromatin Immunoprecipitation: SETD1A Antibody [NB100-558] - Chromatin Immunoprecipitation: SETD1A Antibody [NB100-558] - ChIP-chip scatter plot of anti-hSET1 (NB100-558) enriched DNA binding sites versus input reference DNA. A. 10 mcg of NB100-558 was used to immunoprecipitate chromatin from K562 cells per Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-hSET1 ChIP, normal rabbit IgG showed little enrichment.

Immunoprecipitation: SETD1A Antibody [NB100-558] - Detection of human hSET1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-hSET1 antibody NB100-558 (lot NB100-558-6) used for IP at 6 ug per reaction. hSET1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-558-5) and another rabbit anti-hSET1 antibody. For blotting immunoprecipitated hSET1, the other antibody was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, IHC, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

SETD1A Antibody Summary

• Immunogen: A synthetic peptide which maps to a region between residues 1200 and 1250 of human hSET1 (KIAA0339) using the numbering given in TrEMBL entry O15047 (GeneID 9739).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SETD1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 10 ug
  • Chromatin Immunoprecipitation Sequencing
  • Immunohistochemistry 1:2000 - 1:10000
  • Immunohistochemistry-Paraffin 1:2000 - 1:10000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:10000 - 1:25000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SETD1A Antibody

• histone-lysine N-methyltransferase SETD1A
• hSET1A
• KIAA0339EC 2.1.1.43
• KMT2FSET1
• Lysine N-methyltransferase 2F
• SET domain containing 1A
• SET domain-containing protein 1A
• Set1
• Set1/Ash2 histone methyltransferase complex subunit SET1
• SET1A

Background

SET1A is a component of a histone methyltransferase (HMT) complex that produces mono-, di-, and trimethylated histone H3 at Lys4. The complex is the analog of the S. cerevisiae Set1/COMPASS complex (Lee and Skalnik, 2005 [PubMed 16253997]). Also see SET1B (MIM 611055).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SETD1A Antibody (NB100-558)(5)

Publications using NB100-558

• Wang Z, Yuan H, Roth M et al. SIRT1 deacetylase promotes acquisition of genetic mutations for drug resistance in CML cells. Oncogene 2012-03-01 [PMID: 22410779]
• El Mansouri FE, Chabane N, Zayed N et al. Contribution of H3K4 methylation by SET-1A to interleukin-1-induced cyclooxygenase 2 and inducible nitric oxide synthase expression in human osteoarthritis chondrocytes. Arthritis Rheum 2011-01-01 [PMID: 20862685]
• Jacinto FV, Ballestar E, Esteller M. Impaired recruitment of the histone methyltransferase DOT1L contributes to the incomplete reactivation of tumor suppressor genes upon DNA demethylation. Oncogene 2009-11-26 [PMID: 19734945] (Chemotaxis, Human)
• El-Osta A, Brasacchio D, Yao D et al. Transient high glucose causes persistent epigenetic changes and altered gene expression during subsequent normoglycemia. J Exp Med 2008-09-01 [PMID: 18809715]
• Lee JH, Tate CM, You JS et al. Identification and characterization of the human Set1B histone H3-Lys4 methyltransferase complex. J Biol Chem 2007-05-01 [PMID: 17355966]

Bioinformatics

• Gene Symbol: SETD1A
• Entrez:
  • 9739(Human)
• Uniprot:
  • O15047(Human)