KMT2A/MLL Antibody

Images

 
Western Blot: KMT2A/MLL Antibody [NB600-256] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MLL1 antibody used for WB at 0.1 ...read more
Flow Cytometry: KMT2A/MLL Antibody [NB600-256] - Flow cytometry testing of KMT2A/MLL antibody on murine WT and Mll1-/- CD4 T cells that were stimulated in-vitro with anti-CD3/ -CD28 antibodies. Image submitted by our ...read more
Chromatin Immunoprecipitation: KMT2A/MLL Antibody [NB600-256] - A. 10 ug of was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256).
Independent Antibodies: Immunoprecipitation: KMT2A/MLL Antibody [NB600-256] - Detection of human MLL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ChIP, Flow, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

KMT2A/MLL Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 2725 and 2775 of human myeloid/lymphoid or mixed-lineage leukemia 1 using the number given in Swiss-Prot entry Q03164 (GeneID 4297).
Predicted Species
Mouse (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KMT2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 1-5 ug/ml
  • Flow Cytometry
  • Immunoprecipitation 2-5 ug/mg of lysate
  • Simple Western 1:10
  • Western Blot 1:2000-1:10000
Application Notes
KMT2A/MLL antibody validated for FLOW from a verified customer review. Simple Western reported by an internal validation. Separated by Size, antibody dilution of 1:10
Publications
Read Publications using NB600-256.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for KMT2A/MLL Antibody

  • ALL1
  • ALL-1MLL/GAS7
  • CXXC7TET1-MLL
  • CXXC-type zinc finger protein 7
  • EC 2.1.1.43
  • HRXFLJ11783
  • HTRX
  • HTRX1MLL-AF4 der(11) fusion protein
  • KMT2ACDK6/MLL fusion protein
  • Lysine N-methyltransferase 2A
  • MLL/GAS7 fusion protein
  • MLL/GMPS fusion protein
  • MLL1
  • MLL1A
  • myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog)
  • myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila)
  • Trithorax-like protein
  • TRX1histone-lysine N-methyltransferase MLL
  • Zinc finger protein HRX

Background

A partial tandem duplication within the MLL-gene (MLL-PTD) can be found in 8% of all patients with karyotypically normal acute myeloid leukemia (AML). MLL possibly acts as a transcriptional regulatory factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KMT2A/MLL Antibody (NB600-256)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol

FAQs for KMT2A/MLL Antibody (NB600-256) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KMT2A
Entrez
Uniprot