KLRC1/2/3 Antibody (20d5)

Images

 
Flow Cytometry: KLRC1/2/3 Antibody (20d5) [NBP1-28100] - Analysis using the FITC conjugate of NBP1-28100. Multiple staining of C57BL/6 splenocytes.
Flow Cytometry: KLRC1/2/3 Antibody (20d5) [NBP1-28100] - Analysis using the Biotin conjugate of NBP1-28100. Multiple staining of C57BL/6 splenocytes.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications B/N, Flow
Clone
20d5
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

KLRC1/2/3 Antibody (20d5) Summary

Immunogen
CHO cells transfected with B6 allele of NKG2A gene
Specificity
Mouse NKG2 (Mr approx. 43 kDa)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
KLRC1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Blocking/Neutralizing 50-200 molar excess
  • Flow Cytometry <= 1 ug/10^6 cells

Reactivity Notes

Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for KLRC1/2/3 Antibody (20d5)

  • CD159 antigen-like family member A
  • CD159a antigen
  • CD159a
  • C-lectin type II protein
  • killer cell lectin-like receptor subfamily C, member 1
  • MGC13374
  • natural killer cell lectin
  • natural killer group protein 2
  • NK cell receptor A
  • NKG2
  • NKG2-1/B activating NK receptor
  • NKG2-A
  • NKG2-A/B type II integral membrane protein
  • NKG2-A/B-activating NK receptor
  • NKG2-A/NKG2-B type II integral membrane protein
  • NKG2AMGC59791
  • NKG2-B

Background

The monoclonal antibody 20d5 reacts with an epitope common to the NKG2A, NKG2C and NKG2E isoforms of the CD94/NKG2 heterodimer, a member of the c-type lectin family of inhibitory receptors. Mouse CD94/NKG2 is a natural killer (NK) cell receptor for the non-classical MHC class I molecule Qa-1b. (2, 3) NKG2 is expressed on NK cells, lymphokine-activated killer (LAK) T cells, and some CD8+ memory T cells. Two-color immunofluorescent staining with the 20d5 antibody reveals two distinct NKG2+ phenotypes within the NK1.1+CD3- spleen cell population: NKG2hi (50%) and NKG2low (50%). With the exception of DBA/2J mice, NKG2 is expressed on NK1.1+ cells from all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL). (1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-28100AF405
Alexa Fluor 488 Labeled NBP1-28100AF488
Alexa Fluor 647 Labeled NBP1-28100AF647
Alexa Fluor 700 Labeled NBP1-28100AF700
Allophycocyanin Labeled NBP1-28100APC
Biotin Labeled NBP1-28102
DyLight 350 Labeled NBP1-28100UV
DyLight 405 Labeled NBP1-28100V
DyLight 488 Labeled NBP1-28100G
DyLight 680 Labeled NBP1-28100FR
DyLight 755 Labeled NBP1-28100IR
FITC Labeled NBP1-28101
PE Labeled NBP1-28100PE
PerCP Labeled NBP1-28100PCP

Additional KLRC1/2/3 Products

KLRC1/2/3 NBP1-28100

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Bioinformatics

Gene Symbol KLRC1