KLRC1/2/3 Antibody (20d5) - Azide and BSA Free

Flow Cytometry: KLRC1/2/3 Antibody (20d5) [NBP1-28100] - Analysis using the FITC conjugate of NBP1-28100. Multiple staining of C57BL/6 splenocytes.

• Reactivity: Mu
• Applications: Flow
• Clone: 20d5
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

KLRC1/2/3 Antibody (20d5) - Azide and BSA Free Summary

• Description: Novus Biologicals Rat KLRC1/2/3 Antibody (20d5) - Azide and BSA Free (NBP1-28100) is a monoclonal antibody validated for use in Flow. Anti-KLRC1/2/3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: CHO cells transfected with B6 allele of NKG2A gene
• Specificity: Mouse NKG2 (Mr approx. 43 kDa)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: KLRC1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry <= 1 ug/10^6 cells

Reactivity Notes

Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.1M BBS (pH 8.2)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for KLRC1/2/3 Antibody (20d5) - Azide and BSA Free

• CD159 antigen-like family member A
• CD159a antigen
• CD159A
• C-lectin type II protein
• killer cell lectin-like receptor subfamily C, member 1
• MGC13374
• natural killer cell lectin
• natural killer group protein 2
• NK cell receptor A
• NKG2
• NKG2-1/B activating NK receptor
• NKG2-A
• NKG2-A/B type II integral membrane protein
• NKG2-A/B-activating NK receptor
• NKG2-A/NKG2-B type II integral membrane protein
• NKG2AMGC59791
• NKG2-B

Background

The monoclonal antibody 20d5 reacts with an epitope common to the NKG2A, NKG2C and NKG2E isoforms of the CD94/NKG2 heterodimer, a member of the c-type lectin family of inhibitory receptors. Mouse CD94/NKG2 is a natural killer (NK) cell receptor for the non-classical MHC class I molecule Qa-1b. (2, 3) NKG2 is expressed on NK cells, lymphokine-activated killer (LAK) T cells, and some CD8+ memory T cells. Two-color immunofluorescent staining with the 20d5 antibody reveals two distinct NKG2+ phenotypes within the NK1.1+CD3- spleen cell population: NKG2hi (50%) and NKG2low (50%). With the exception of DBA/2J mice, NKG2 is expressed on NK1.1+ cells from all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL). (1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KLRC1/2/3 Antibody (NBP1-28100)(1)

Publication using NBP1-28100

• Webb ER, Moreno-Vincente J, Easton A Et al. Cyclophosphamide depletes tumor infiltrating T regulatory cells and combined with anti-PD-1 therapy improves survival in murine neuroblastoma iScience 2022-09-13 [PMID: 36097618] (FLOW, Mouse)
  
  Details:
  Citation using the PE version of this antibody.

Bioinformatics

• Gene Symbol: KLRC1