NKp46/NCR1 Antibody [Unconjugated]

Images

 
Mouse NKp46/NCR1 Antigen Affinity-purified Polyclonal Antibody induces IFN-gamma secretion in mouse natural killer (NK) cells activated with 25 ng/mL Recombinant Mouse IL-2 (Catalog # 402-ML) and 25 ng/mL Recombinant ...read more
NKp46/NCR1 was detected in perfusion fixed frozen sections of mouse spleen using Goat Anti-Mouse NKp46/NCR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2225) at 3 µg/mL for 1 hour at room ...read more
Mouse DX5/CD49b+ splenocytes were stained with Goat Anti-Mouse NKp46/NCR1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2225, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, Func
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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NKp46/NCR1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse NKp46/NCR1
Glu22-Asn255
Accession # Q8C567
Specificity
Detects mouse NKp46/NCR1 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human (rh) NKp46 is observed.
Details of Functionality
ActivityAssaywCitation not found None None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NCR1
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 3-15 ug/mL
  • Agonist Activity 0.4-2.4 ug/mL
  • CyTOF-ready
Reviewed Applications
Read 1 Review rated 5
using
AF2225 in the following applications:

Publications
Read Publications using
AF2225 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKp46/NCR1 Antibody [Unconjugated]

  • CD335 antigen
  • CD335
  • hNKp46
  • Ly94
  • LY94lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46)
  • Lymphocyte antigen 94 homolog
  • MAR-1
  • natural cytotoxicity triggering receptor 1
  • Natural killer cell p46-related protein
  • NCR1
  • NK cell-activating receptor
  • NKp46
  • NKP46FLJ99094
  • NK-p46lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)

Background

NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. In human systems, the CD56dimCD16+ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56brightCD16-) expresses low levels of NKp46 in resting cells, but expression is upregulated by IL-2. Mouse NKp46, also known as MAR-1 (3), is a type I transmembrane protein with two extracellular Ig-like domains. It has a positive charge in its transmembrane domain that permits association with the ITAM-bearing signal adapter proteins, CD3 zeta and Fc epsilon RI gamma (4). Studies with neutralizing antibodies indicate that the three NCR are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (5). NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (6-8).

  1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
  2. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
  3. Biassoni, R. et al. (1999) Eur. J. Immunol. 29:1014.
  4. Westgaard, I. et al. (2004) J. Leukoc. Biol. PMID 15356098.
  5. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
  6. Arnon, T. et al. (2004) Blood 103:664.
  7. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.
  8. Mandelboim, O. et al. (2001) Nature 409:1055.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, IHC, CyTOF-ready, Func

Publications for NKp46/NCR1 Antibody (AF2225)(26)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: Bioassay, Flow Cytometry, Functional Assay, IHC, IHC-Fr, IHC-P, Neutralization.


Filter By Application
Bioassay
(2)
Flow Cytometry
(5)
Functional Assay
(4)
IHC
(4)
IHC-Fr
(7)
IHC-P
(2)
Neutralization
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(23)
Rat
(2)
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Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using AF2225 Applications Species
S Nishina, A Yamauchi, T Kawaguchi, K Kaku, M Goto, K Sasaki, Y Hara, Y Tomiyama, F Kuribayash, T Torimura, K Hino Dipeptidyl Peptidase 4 Inhibitors Reduce Hepatocellular Carcinoma by Activating Lymphocyte Chemotaxis in Mice Cell Mol Gastroenterol Hepatol, 2019;7(1):115-134. 2019 [PMID: 30510994] (IHC-P, Mouse) IHC-P Mouse
N Kikuchi, J Ye, J Hirakawa, H Kawashima Forced expression of CXCL10 prevents liver metastasis of colon carcinoma cells by the recruitment of natural killer cells Biol. Pharm. Bull., 2018;0(0):. 2018 [PMID: 30381616] (IHC, Mouse) IHC Mouse
F Janker, W Weder, JH Jang, W Jungraithm Preclinical, non-genetic models of lung adenocarcinoma: a comparative survey Oncotarget, 2018;9(55):30527-30538. 2018 [PMID: 30093966] (IHC, Mouse) IHC Mouse
EE Er, M Valiente, K Ganesh, Y Zou, S Agrawal, J Hu, B Griscom, M Rosenblum, A Boire, E Brogi, FG Giancotti, M Schachner, S Malladi, J Massagué Pericyte-like spreading by disseminated cancer cells activates YAP and MRTF for metastatic colonization Nat. Cell Biol., 2018;20(8):966-978. 2018 [PMID: 30038252] (IHC, Mouse) IHC Mouse
KB Singh, X Ji, SV Singh Therapeutic Potential of Leelamine, a Novel Inhibitor of Androgen Receptor and Castration-Resistant Prostate Cancer Mol. Cancer Ther., 2018;0(0):. 2018 [PMID: 30030299] (Flow Cytometry, Rat) Flow Cytometry Rat
A Martin, C Seignez, C Racoeur, N Isambert, Isambert N, Mabrouk N, N Mabrouk, A Scagliarin, Scagliarini A, Reveneau S, S Reveneau, L Arnould, Arnould L, A Bettaieb, JF Jeannin, Jeannin J, C Paul Tumor-derived granzyme B-expressing neutrophils acquire antitumor potential after lipid A treatment. Oncotarget, 0;9(47):28364-28378. 0 [PMID: 29983866] (Flow Cytometry, Rat) Flow Cytometry Rat
G Turchinovi, S Ganter, A Bärenwaldt, D Finke NKp46 Calibrates Tumoricidal Potential of Type 1 Innate Lymphocytes by Regulating TRAIL Expression J. Immunol., 2018;0(0):. 2018 [PMID: 29661825] (Bioassay, Mouse) Bioassay Mouse
EM Putz, AJ Mayfosh, K Kos, DS Barkauskas, K Nakamura, L Town, KJ Goodall, DY Yee, IK Poon, N Baschuk, F Souza-Fons, MD Hulett, MJ Smyth NK cell heparanase controls tumor invasion and immune surveillance J. Clin. Invest., 2017;0(0):. 2017 [PMID: 28581441] (IHC-Fr, Mouse) IHC-Fr Mouse
SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice J. Immunol, 2017;0(0):. 2017 [PMID: 28219886] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Independent control of natural killer cell responsiveness and homeostasis at steady-state by CD11c+ dendritic cells Sci Rep, 2016;6(0):37996. 2016 [PMID: 27905484] (Functional Assay, Mouse) Functional Assay Mouse
Show All 26 Publications.

Review for NKp46/NCR1 Antibody (AF2225) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF2225:
Filter by Applications
IHC-Fr
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-Fr Mouse 01/06/2015
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 23902341
SpeciesMouse

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Secondary Antibodies

 

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Other Available Formats

Biotin BAF2225
Fluorescein FAB2225F
Fluorescein FAB2225F

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Recent Reviews

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Anonymous
01/06/2015
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol NCR1
Uniprot