NKp46/NCR1 Antibody [Unconjugated]

Mouse NKp46/NCR1 Antigen Affinity-purified Polyclonal Antibody induces IFN-gamma secretion in mouse natural killer (NK) cells activated with 25 ng/mL Recombinant Mouse IL-2 (Catalog # 402-ML) and 25 ng/mL Recombinant Mouse IL-12 (Catalog # 419-ML), in a dose-dependent manner, as measured using the Quantikine Mouse IFN-gamma ELISA Kit (Catalog # MIF00). The ED50 for this effect is typically0.4-2.4 μg/mL.

Mouse DX5/CD49b+ splenocytes were stained with Goat Anti-Mouse NKp46/NCR1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2225, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

NKp46/NCR1 was detected in perfusion fixed frozen sections of mouse spleen using Goat Anti-Mouse NKp46/NCR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2225) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Mu
• Applications: WB, Flow, IHC, CyTOF-ready, Func
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

NKp46/NCR1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse NKp46/NCR1
  Glu22-Asn255
  Accession # Q8C567
• Specificity: Detects mouse NKp46/NCR1 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human (rh) NKp46 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NCR1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Agonist Activity 0.4-2.4 ug/mL
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKp46/NCR1 Antibody [Unconjugated]

• CD335 antigen
• CD335
• hNKp46
• Ly94
• LY94lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46)
• Lymphocyte antigen 94 homolog
• MAR-1
• natural cytotoxicity triggering receptor 1
• Natural killer cell p46-related protein
• NCR1
• NK cell-activating receptor
• NKp46
• NKP46FLJ99094
• NK-p46lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)

Background

NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. In human systems, the CD56^dimCD16⁺ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56^brightCD16^-) expresses low levels of NKp46 in resting cells, but expression is upregulated by IL-2. Mouse NKp46, also known as MAR-1 (3), is a type I transmembrane protein with two extracellular Ig-like domains. It has a positive charge in its transmembrane domain that permits association with the ITAM-bearing signal adapter proteins, CD3 zeta and Fc epsilon RI gamma (4). Studies with neutralizing antibodies indicate that the three NCR are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (5). NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (6-8).

1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
2. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
3. Biassoni, R. et al. (1999) Eur. J. Immunol. 29:1014.
4. Westgaard, I. et al. (2004) J. Leukoc. Biol. PMID 15356098.
5. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
6. Arnon, T. et al. (2004) Blood 103:664.
7. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.
8. Mandelboim, O. et al. (2001) Nature 409:1055.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NKp46/NCR1 Antibody (AF2225)(31)

Publications using AF2225

• B Xu, L Tian, J Chen, J Wang, R Ma, W Dong, A Li, J Zhang, E Antonio Ch, B Kaur, M Feng, MA Caligiuri, J Yu An oncolytic virus expressing a full-length antibody enhances antitumor innate immune response to glioblastoma Nature Communications, 2021;12(1):5908. 2021 [PMID: 34625564] (IHC, Mouse)
• WN Jin, K Shi, W He, JH Sun, L Van Kaer, FD Shi, Q Liu Neuroblast senescence in the aged brain augments natural killer cell cytotoxicity leading to impaired neurogenesis and cognition Nature Neuroscience, 2020;0(0):. 2020 [PMID: 33257875] (IHC, Mouse)
• FM Kobia, K Preusse, Q Dai, N Weaver, MR Hass, P Chaturvedi, SJ Stein, WS Pear, Z Yuan, RA Kovall, Y Kuang, N Eafergen, D Sprinzak, B Gebelein, EW Brunskill, R Kopan Notch dimerization and gene dosage are important for normal heart development, intestinal stem cell maintenance, and splenic marginal zone B-cell homeostasis during mite infestation PLoS Biol, 2020;18(10):e3000850. 2020 [PMID: 33017398] (Western Blot, Mouse)
• A Hargrave, JA Courson, V Pham, P Landry, S Magadi, P Shankar, S Hanlon, A Das, RE Rumbaut, CW Smith, AR Burns Corneal dysfunction precedes the onset of hyperglycemia in a mouse model of diet-induced obesity PLoS ONE, 2020;15(9):e0238750. 2020 [PMID: 32886728] (IHC, Mouse)
• J Yun, SH Lee, SY Kim, SY Jeong, JH Kim, KH Pyo, CW Park, SG Heo, MR Yun, S Lim, SM Lim, MH Hong, HR Kim, M Thayu, JC Curtin, RE Knoblauch, MV Lorenzi, A Roshak, BC Cho Antitumor Activity of Amivantamab (JNJ-61186372), an EGFR-cMet Bispecific Antibody, in Diverse Models of EGFR Exon 20 Insertion-Driven NSCLC Cancer Discov, 2020;0(0):. 2020 [PMID: 32414908] (IHC, Mouse)
• S Nishina, A Yamauchi, T Kawaguchi, K Kaku, M Goto, K Sasaki, Y Hara, Y Tomiyama, F Kuribayash, T Torimura, K Hino Dipeptidyl Peptidase 4 Inhibitors Reduce Hepatocellular Carcinoma by Activating Lymphocyte Chemotaxis in Mice Cell Mol Gastroenterol Hepatol, 2019;7(1):115-134. 2019 [PMID: 30510994] (IHC-P, Mouse)
• N Kikuchi, J Ye, J Hirakawa, H Kawashima Forced expression of CXCL10 prevents liver metastasis of colon carcinoma cells by the recruitment of natural killer cells Biol. Pharm. Bull., 2018;0(0):. 2018 [PMID: 30381616] (IHC, Mouse)
• F Janker, W Weder, JH Jang, W Jungraithm Preclinical, non-genetic models of lung adenocarcinoma: a comparative survey Oncotarget, 2018;9(55):30527-30538. 2018 [PMID: 30093966] (IHC, Mouse)
• EE Er, M Valiente, K Ganesh, Y Zou, S Agrawal, J Hu, B Griscom, M Rosenblum, A Boire, E Brogi, FG Giancotti, M Schachner, S Malladi, J Massagué Pericyte-like spreading by disseminated cancer cells activates YAP and MRTF for metastatic colonization Nat. Cell Biol., 2018;20(8):966-978. 2018 [PMID: 30038252] (IHC, Mouse)
• KB Singh, X Ji, SV Singh Therapeutic Potential of Leelamine, a Novel Inhibitor of Androgen Receptor and Castration-Resistant Prostate Cancer Mol. Cancer Ther., 2018;0(0):. 2018 [PMID: 30030299] (Flow Cytometry, Rat)
• A Martin, C Seignez, C Racoeur, N Isambert, Isambert N, Mabrouk N, N Mabrouk, A Scagliarin, Scagliarini A, Reveneau S, S Reveneau, L Arnould, Arnould L, A Bettaieb, JF Jeannin, Jeannin J, C Paul Tumor-derived granzyme B-expressing neutrophils acquire antitumor potential after lipid A treatment. Oncotarget, 0;9(47):28364-28378. 0 [PMID: 29983866] (Flow Cytometry, Rat)
• G Turchinovi, S Ganter, A Bärenwaldt, D Finke NKp46 Calibrates Tumoricidal Potential of Type 1 Innate Lymphocytes by Regulating TRAIL Expression J. Immunol., 2018;0(0):. 2018 [PMID: 29661825] (Bioassay, Mouse)
• EM Putz, AJ Mayfosh, K Kos, DS Barkauskas, K Nakamura, L Town, KJ Goodall, DY Yee, IK Poon, N Baschuk, F Souza-Fons, MD Hulett, MJ Smyth NK cell heparanase controls tumor invasion and immune surveillance J. Clin. Invest., 2017;0(0):. 2017 [PMID: 28581441] (IHC-Fr, Mouse)
• SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice J. Immunol, 2017;0(0):. 2017 [PMID: 28219886] (Flow Cytometry, Mouse)
• Independent control of natural killer cell responsiveness and homeostasis at steady-state by CD11c+ dendritic cells Sci Rep, 2016;6(0):37996. 2016 [PMID: 27905484] (Functional Assay, Mouse)
• Murine liver-resident group 1 innate lymphoid cells regulate optimal priming of anti-viral CD8+ T cells J Leukoc Biol, 2016;0(0):. 2016 [PMID: 27493244] (IHC, Mouse)
• IL-2 in the tumor microenvironment is necessary for Wiskott-Aldrich syndrome protein deficient NK cells to respond to tumors in vivo Sci Rep, 2016;6(0):30636. 2016 [PMID: 27477778] (Functional Assay, Mouse)
• W Rabacal, SK Pabbisetty, KL Hoek, D Cendron, Y Guo, D Maseda, E Sebzda Transcription factor KLF2 regulates homeostatic NK cell proliferation and survival Proc Natl Acad Sci USA, 2016;0(0):. 2016 [PMID: 27114551] (IHC-Fr, Mouse)
• Guo Y, Luan L, Rabacal W, Bohannon J, Fensterheim B, Hernandez A, Sherwood E IL-15 Superagonist-Mediated Immunotoxicity: Role of NK Cells and IFN-gamma. J Immunol, 2015;195(5):2353-64. 2015 [PMID: 26216888] (IHC-Fr, Mouse)
• Spada R, Rojas J, Perez-Yague S, Mulens V, Cannata-Ortiz P, Bragado R, Barber D NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery. J Leukoc Biol, 2015;97(3):583-98. 2015 [PMID: 25583577] (IHC-P, Human)
• Mracsko E, Liesz A, Stojanovic A, Lou W, Osswald M, Zhou W, Karcher S, Winkler F, Martin-Villalba A, Cerwenka A, Veltkamp R Antigen dependently activated cluster of differentiation 8-positive T cells cause perforin-mediated neurotoxicity in experimental stroke. J Neurosci, 2014;34(50):16784-95. 2014 [PMID: 25505331] (Bioassay, Mouse)
• Li C, Yang Z, Du Y, Tang H, Chen J, Hu D, Fan Z BCMab1, a monoclonal antibody against aberrantly glycosylated integrin alpha3beta1, has potent antitumor activity of bladder cancer in vivo. Clin Cancer Res, 2014;20(15):4001-13. 2014 [PMID: 25002124] (Mouse)
• Stolberg V, Martin B, Mancuso P, Olszewski M, Freeman C, Curtis J, Chensue S Role of CC chemokine receptor 4 in natural killer cell activation during acute cigarette smoke exposure. Am J Pathol, 2014;184(2):454-63. 2014 [PMID: 24333113] (IHC-Fr, Mouse)
• Biroccio A, Cherfils-Vicini J, Augereau A, Pinte S, Bauwens S, Ye J, Simonet T, Horard B, Jamet K, Cervera L, Mendez-Bermudez A, Poncet D, Grataroli R, de Rodenbeeke C, Salvati E, Rizzo A, Zizza P, Ricoul M, Cognet C, Kuilman T, Duret H, Lepinasse F, Marvel J, Verhoeyen E, Cosset F, Peeper D, Smyth M, Londono-Vallejo A, Sabatier L, Picco V, Pages G, Scoazec J, Stoppacciaro A, Leonetti C, Vivier E, Gilson E TRF2 inhibits a cell-extrinsic pathway through which natural killer cells eliminate cancer cells. Nat Cell Biol, 2013;15(7):818-28. 2013 [PMID: 23792691] (IHC-Fr, Mouse)
• Klezovich-Benard M, Corre JP, Jusforgues-Saklani H, Fiole D, Burjek N, Tournier JN, Goossens PL Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins. PLoS Pathog., 2012;8(1):e1002481. 2012 [PMID: 22253596] (Neutralization, Mouse)
• Gur C, Enk J, Kassem SA, Suissa Y, Magenheim J, Stolovich-Rain M, Nir T, Achdout H, Glaser B, Shapiro J, Naparstek Y, Porgador A, Dor Y, Mandelboim O Recognition and killing of human and murine pancreatic beta cells by the NK receptor NKp46. J. Immunol., 2011;187(6):3096-103. 2011 [PMID: 21849674] (IHC-Fr, Mouse)
• Eidenschenk C, Crozat K, Krebs P, Arens R, Popkin D, Arnold CN, Blasius AL, Benedict CA, Moresco EM, Xia Y, Beutler B Flt3 permits survival during infection by rendering dendritic cells competent to activate NK cells. Proc. Natl. Acad. Sci. U.S.A., 2010;107(21):9759-64. 2010 [PMID: 20457904] (Functional Assay, Mouse)
• Brauner H, Elemans M, Lemos S, Broberger C, Holmberg D, Flodstrom-Tullberg M, Karre K, Hoglund P Distinct phenotype and function of NK cells in the pancreas of nonobese diabetic mice. J. Immunol., 2010;184(5):2272-80. 2010 [PMID: 20130214] (Functional Assay, Mouse)
• Luangsay S, Wittamer V, Bondue B, De Henau O, Rouger L, Brait M, Franssen JD, de Nadai P, Huaux F, Parmentier M Mouse ChemR23 is expressed in dendritic cell subsets and macrophages, and mediates an anti-inflammatory activity of chemerin in a lung disease model. J. Immunol., 2009;183(10):6489-99. 2009 [PMID: 19841182] (Flow Cytometry, Mouse)
• Kang SJ, Liang HE, Reizis B, Locksley RM Regulation of hierarchical clustering and activation of innate immune cells by dendritic cells. Immunity, 2008;29(5):819-33. 2008 [PMID: 19006696] (IHC-Fr, Mouse)
• Vosshenrich CA, Lesjean-Pottier S, Hasan M, Richard-Le Goff O, Corcuff E, Mandelboim O, Di Santo JP CD11cloB220+ interferon-producing killer dendritic cells are activated natural killer cells. J. Exp. Med., 2007;204(11):2569-78. 2007 [PMID: 17923507] (Flow Cytometry, Mouse)

Review for NKp46/NCR1 Antibody (AF2225) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Anonymous - IHC-Fr Mouse 01/06/2015

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: See PMID 23902341
• Species: Mouse

Bioinformatics

• Gene Symbol: NCR1
• Uniprot:
  • Q8C567()