ILT2/CD85j/LILRB1 Antibody (292319) [Unconjugated]


Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (Catalog # 2017-T2), immobilized onto a microplate, supports the adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cell line in a dose-dependent more

Product Details

Reactivity HuSpecies Glossary
Applications WB, B/N, CyTOF-reported

Order Details

View Available Formulations
Catalog# & Formulation Size Price

ILT2/CD85j/LILRB1 Antibody (292319) [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant human LILRB1/CD85j/ILT2
Accession # Q8NHL6
Detects human LILRB1/CD85j/ILT2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ILT1, 3, 4, 5, rhLIR-6 or rhLIR-8 is observed.
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1 ug/mL
  • CyTOF-reported
  • Neutralization 0.1-0.3 ug/mL
Read Publications using
MAB20172 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ILT2/CD85j/LILRB1 Antibody (292319) [Unconjugated]

  • CD85 antigen-like family member J
  • CD85j antigen
  • CD85j
  • Ig-like transcript 2
  • ILT2
  • ILT-2
  • ILT2FLJ37515
  • Immunoglobulin-like transcript 2
  • leukocyte Ig-like receptor-1
  • Leukocyte immunoglobulin-like receptor 1
  • leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform
  • leukocyte immunoglobulin-like receptor subfamily B member 1
  • leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains)
  • LILRB1
  • LIR1
  • LIR1LIR-1MIR-7CD85MIR7CD85 antigen
  • member 1
  • MIR7
  • Monocyte/macrophage immunoglobulin-like receptor 7


The immunoglobulin-like transcript (ILT) family of activating and inhibitory type immunoreceptors are expressed on many leukocyte subsets and function in the regulation of immune responses (1‑3). This family was also named leukocyte Ig-like receptors (LIR) and monocyte/macrophage Ig-like receptors (MIR). ILTs share significant homology with killer cell Ig-like receptors (KIR). The ILT genes are located on human chromosome 19q13.4 in the leukocyte receptor complex, which also include the genes encoding KIRs (4). With the exception of ILT-6, which is a soluble molecule, all ILT family members are type I transmembrane proteins having two or four extracellular Ig-like domains (2, 3). One subset of the ILT receptors (referred to as subfamily B of the LIRs) has long cytoplasmic tails containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events by recruiting SH2-containing protein tyrosine phosphatase-1. Another subset of the ILT receptors (referred to as subfamily A of the LIRs) contains activating receptors with short cytoplasmic regions that lack signal transduction motifs. These receptors contain a basic arginine residue within their transmembrane domains, which allows association with Fc R gamma, an immunoreceptor tyrosine-based activation motif (ITAM)-bearing signal adapter protein (1‑3). ILT2, also known as LIR1, MIR7, and CD85j, is expressed on most monocytes, dendritic cells, and mature B cells (1‑3). It is also expressed on small percentages of T cells and NK cells. ILT2 has four extracellular Ig-like domains and three cytoplasmic ITIMs. It functions as an inhibitory receptor that prevents cellular activation. ILT2 has been shown to bind classical (HLA-A and -B) and nonclassical (HLA-G1, -E and -F) MHC class I molecules (MHCI) (1‑3). ILT2 also binds with high affinity to an MHC class I homologue from human cytomegalovirus (3). Ligation of ILT2 by MHC class I may function to poise cellular activation thresholds and inhibit various leukocyte effector mechanisms that are regulated by MHC class I molecules on target cells.

  1. Allen, D. et al. (2000) Immunobiol. 202:34.
  2. Colonna, M. et al. (1999) J. Leukocyte Biol. 66:375.
  3. Borges, L. and D. Cosman (2000) Cytokine Growth Factor Rev. 11:209.
  4. Young, N. et al. (2001) Immunogenetics 53:270.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
Species: Hu, Po
Applications: Flow, IHC, IHC-Fr, IF
Species: Hu
Applications: WB, Flow, CyTOF-ready, Neut
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, B/N, CyTOF-ready, Flow-CS
Species: Hu
Applications: Flow, Block, CyTOF-ready
Species: Hu
Applications: Flow, CyTOF-ready
Species: Rt
Applications: EM, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N, CyTOF-ready, Flow-IC
Species: Hu
Applications: Flow, CyTOF-ready
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, Flow, CyTOF-ready
Species: Hu
Applications: WB
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
Species: Hu
Applications: Flow, AgAct, CyTOF-reported
Species: Hu
Applications: WB, Flow, CostimT, CyTOF-ready, Neut
Species: Hu
Applications: WB, B/N, CyTOF-reported

Publications for ILT2/CD85j/LILRB1 Antibody (MAB20172)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: CyTof, Functional Assay, Neutralization.

Filter By Application
Functional Assay
All Applications
Filter By Species
All Species
Showing Publications 1 - 5 of 5.
Publications using MAB20172 Applications Species
FG Karnell, D Lin, S Motley, T Duhen, N Lim, DJ Campbell, LA Turka, HT Maecker, KM Harris Reconstitution of immune cell populations in multiple sclerosis patients after autologous stem cell transplantation Clin. Exp. Immunol., 2017;0(0):. 2017 [PMID: 28498568] (CyTof, Human) CyTof Human
Mei H, Leipold M, Schulz A, Chester C, Maecker H Barcoding of live human peripheral blood mononuclear cells for multiplexed mass cytometry. J Immunol, 2015;194(4):2022-31. 2015 [PMID: 25609839] (CyTof, Human) CyTof Human
Arguello RJ, Albareda MC, Alvarez MG, Bertocchi G, Armenti AH, Vigliano C, Meckert PC, Tarleton RL, Laucella SA Inhibitory receptors are expressed by Trypanosoma cruzi-specific effector T cells and in hearts of subjects with chronic Chagas disease. PLoS ONE, 2012;7(5):e35966. 2012 [PMID: 22574131] (Functional Assay, Human) Functional Assay Human
Godal R, Bachanova V, Gleason M Natural killer cell killing of acute myelogenous leukemia and acute lymphoblastic leukemia blasts by killer cell immunoglobulin-like receptor-negative natural killer cells after NKG2A and LIR-1 blockade. Biol. Blood Marrow Transplant., 2010;16(5):612-21. 2010 [PMID: 20139023] (Neutralization, Human) Neutralization Human
Coupel S, Moreau A, Hamidou M, Horejsi V, Soulillou JP, Charreau B Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation. Blood, 2007;109(7):2806-14. 2007 [PMID: 17179229] (Neutralization, Human) Neutralization Human

Reviews for ILT2/CD85j/LILRB1 Antibody (MAB20172) (0)

There are no reviews for ILT2/CD85j/LILRB1 Antibody (MAB20172). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ILT2/CD85j/LILRB1 Antibody (MAB20172) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Other Available Formats

Additional ILT2/CD85j/LILRB1 Products

Bioinformatics Tool for ILT2/CD85j/LILRB1 Antibody (MAB20172)

Discover related pathways, diseases and genes to ILT2/CD85j/LILRB1 Antibody (MAB20172). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ILT2/CD85j/LILRB1 Antibody (MAB20172)

Discover more about diseases related to ILT2/CD85j/LILRB1 Antibody (MAB20172).

Pathways for ILT2/CD85j/LILRB1 Antibody (MAB20172)

View related products by pathway.

PTMs for ILT2/CD85j/LILRB1 Antibody (MAB20172)

Learn more about PTMs related to ILT2/CD85j/LILRB1 Antibody (MAB20172).

Blogs on ILT2/CD85j/LILRB1

There are no specific blogs for ILT2/CD85j/LILRB1, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our ILT2/CD85j/LILRB1 Antibody (292319) [Unconjugated] and receive a gift card or discount.


Gene Symbol LILRB1