ILT2/CD85j/LILRB1 Antibody (292319) [Unconjugated]

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Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (Catalog # 2017-T2), immobilized onto a microplate, supports the adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cell line in a dose-dependent ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, CyTOF-reported
Clone
292319
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

ILT2/CD85j/LILRB1 Antibody (292319) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human LILRB1/CD85j/ILT2
Gly24-His458
Accession # Q8NHL6
Specificity
Detects human LILRB1/CD85j/ILT2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ILT1, 3, 4, 5, rhLIR-6 or rhLIR-8 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
LILRB1
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • CyTOF-reported
  • Neutralization 0.1-0.3 ug/mL
Publications
Read Publications using
MAB20172 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ILT2/CD85j/LILRB1 Antibody (292319) [Unconjugated]

  • CD85 antigen-like family member J
  • CD85j antigen
  • CD85j
  • Ig-like transcript 2
  • ILT2
  • ILT-2
  • ILT2FLJ37515
  • Immunoglobulin-like transcript 2
  • leukocyte Ig-like receptor-1
  • Leukocyte immunoglobulin-like receptor 1
  • leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform
  • leukocyte immunoglobulin-like receptor subfamily B member 1
  • leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains)
  • LILRB1
  • LIR1
  • LIR1LIR-1MIR-7CD85MIR7CD85 antigen
  • member 1
  • MIR7
  • Monocyte/macrophage immunoglobulin-like receptor 7

Background

The immunoglobulin-like transcript (ILT) family of activating and inhibitory type immunoreceptors are expressed on many leukocyte subsets and function in the regulation of immune responses (1‑3). This family was also named leukocyte Ig-like receptors (LIR) and monocyte/macrophage Ig-like receptors (MIR). ILTs share significant homology with killer cell Ig-like receptors (KIR). The ILT genes are located on human chromosome 19q13.4 in the leukocyte receptor complex, which also include the genes encoding KIRs (4). With the exception of ILT-6, which is a soluble molecule, all ILT family members are type I transmembrane proteins having two or four extracellular Ig-like domains (2, 3). One subset of the ILT receptors (referred to as subfamily B of the LIRs) has long cytoplasmic tails containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events by recruiting SH2-containing protein tyrosine phosphatase-1. Another subset of the ILT receptors (referred to as subfamily A of the LIRs) contains activating receptors with short cytoplasmic regions that lack signal transduction motifs. These receptors contain a basic arginine residue within their transmembrane domains, which allows association with Fc R gamma, an immunoreceptor tyrosine-based activation motif (ITAM)-bearing signal adapter protein (1‑3). ILT2, also known as LIR1, MIR7, and CD85j, is expressed on most monocytes, dendritic cells, and mature B cells (1‑3). It is also expressed on small percentages of T cells and NK cells. ILT2 has four extracellular Ig-like domains and three cytoplasmic ITIMs. It functions as an inhibitory receptor that prevents cellular activation. ILT2 has been shown to bind classical (HLA-A and -B) and nonclassical (HLA-G1, -E and -F) MHC class I molecules (MHCI) (1‑3). ILT2 also binds with high affinity to an MHC class I homologue from human cytomegalovirus (3). Ligation of ILT2 by MHC class I may function to poise cellular activation thresholds and inhibit various leukocyte effector mechanisms that are regulated by MHC class I molecules on target cells.

  1. Allen, D. et al. (2000) Immunobiol. 202:34.
  2. Colonna, M. et al. (1999) J. Leukocyte Biol. 66:375.
  3. Borges, L. and D. Cosman (2000) Cytokine Growth Factor Rev. 11:209.
  4. Young, N. et al. (2001) Immunogenetics 53:270.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ILT2/CD85j/LILRB1 Antibody (MAB20172)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: CyTof, Functional Assay, Neutralization.


Filter By Application
CyTof
(2)
Functional Assay
(1)
Neutralization
(2)
All Applications
Filter By Species
Human
(5)
All Species
Showing Publications 1 - 5 of 5.
Publications using MAB20172 Applications Species
FG Karnell, D Lin, S Motley, T Duhen, N Lim, DJ Campbell, LA Turka, HT Maecker, KM Harris Reconstitution of immune cell populations in multiple sclerosis patients after autologous stem cell transplantation Clin. Exp. Immunol., 2017;0(0):. 2017 [PMID: 28498568] (CyTof, Human) CyTof Human
Mei H, Leipold M, Schulz A, Chester C, Maecker H Barcoding of live human peripheral blood mononuclear cells for multiplexed mass cytometry. J Immunol, 2015;194(4):2022-31. 2015 [PMID: 25609839] (CyTof, Human) CyTof Human
Arguello RJ, Albareda MC, Alvarez MG, Bertocchi G, Armenti AH, Vigliano C, Meckert PC, Tarleton RL, Laucella SA Inhibitory receptors are expressed by Trypanosoma cruzi-specific effector T cells and in hearts of subjects with chronic Chagas disease. PLoS ONE, 2012;7(5):e35966. 2012 [PMID: 22574131] (Functional Assay, Human) Functional Assay Human
Godal R, Bachanova V, Gleason M Natural killer cell killing of acute myelogenous leukemia and acute lymphoblastic leukemia blasts by killer cell immunoglobulin-like receptor-negative natural killer cells after NKG2A and LIR-1 blockade. Biol. Blood Marrow Transplant., 2010;16(5):612-21. 2010 [PMID: 20139023] (Neutralization, Human) Neutralization Human
Coupel S, Moreau A, Hamidou M, Horejsi V, Soulillou JP, Charreau B Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation. Blood, 2007;109(7):2806-14. 2007 [PMID: 17179229] (Neutralization, Human) Neutralization Human

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Bioinformatics

Gene Symbol LILRB1
Uniprot