Klotho Antibody

Immunohistochemistry-Paraffin: Klotho Antibody [NB100-2432] - Staining of Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Immunohistochemistry-Paraffin: Klotho Antibody [NB100-2432] - Staining of Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu, Mu, Rt
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Klotho Antibody Summary

• Description: Novus Biologicals Goat Klotho Antibody (NB100-2432) is a polyclonal antibody validated for use in IHC and ELISA. Anti-Klotho Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KRDDAKYMYYLKK corresponding to internal region according to NP_004786.2, NP_710150.1.
• Specificity: This antibody is expected to recognise both reported isoforms (NP_004786.2; NP_710150.1).
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4 - 6 ug/ml
  • Peptide ELISA Detection limit 1:4000
• Application Notes: WB: Preliminary experiments in human brain, duodenum, placenta and prostate lysates gave no specific signal but low background. IHC-P: Human breast shows cytoplasm staining in luminal epithelial cells of ducts, while human placenta shows membranous staining.
• Control:

  Klotho Overexpression Lysate

• Control Peptide:

  Klotho Protein (NB100-2432PEP)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Klotho Antibody

• EC 3.2.1
• EC 3.2.1.31
• KL
• Klotho

Background

The Klotho gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Klotho Antibody (NB100-2432)(1)

Publication using NB100-2432

• Kurosu H, Yamamoto M, Clark JD et al. Suppression of ageing in mice by the hormone Klotho. Science 2005-09-16 [PMID: 16123266]

FAQs for Klotho Antibody (NB100-2432). (Showing 1 - 2 of 2 FAQ).

1. I study physiology (PhD) and I need a klotho kit.
   • A list of our klotho products can be found here. I am not entirely sure what you are looking for as I don't have your complete message for some reason. Can you please clarify what you are looking for?
2. Upon the customer's inquiry, may we ask if there is any product specific reference available for NB100-2432?
   • For all of our products if there is not a reference listed on the data sheet under the product specific reference section we have not found one at this time and do not have one to provide. We are always updating our references as we come across them and will display them on the website if it is available.

Bioinformatics

• Gene Symbol: KL
• Entrez:
  • 9365(Human)
• Uniprot:
  • Q9UEF7()