Recombinant Mouse Flt-3 Ligand/FLT3L Protein

Recombinant Mouse Flt-3 Ligand/FLT3L (Catalog # 427-FL) stimulates cell proliferation in the BaF3 mouse pro-B cell line transfected with mouse Flt-3. The ED50 for this effect is 0.4-2.4 ng/mL.

1 μg/lane of Recombinant Mouse Flt-3 Ligand/FLT3L was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing multiple bands between 18-32 kDa. Multiple bands are due to variable glycosylation of the protein.

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse Flt-3 Ligand/FLT3L Protein Summary

• Details of Functionality: Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with mouse Flt-3. The ED₅₀ for this effect is typically 0.4-2.4 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse Flt-3 Ligand/FLT3L protein
  Gly27-Arg188
• Accession #: P49772.1
• N-terminal Sequence: Gly27
• Protein/Peptide Type: Recombinant Proteins
• Gene: Flt3l
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: Multiple bands between 18-32 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Flt-3 Ligand/FLT3L Protein

• FL
• FLG3L
• Flt3 ligand
• Flt-3 Ligand
• Flt3L
• FLT3LG
• fms-related tyrosine kinase 3 ligand
• SL cytokine

Background

Flt-3 Ligand, also known as FLT3L, is an alpha-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages (1-3). Mature mouse Flt‑3 Ligand consists of a 161 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 22 aa cytoplasmic tail (4-6). Within the ECD, mouse Flt-3 Ligand shares 71% and 81% aa sequence identity with human and rat Flt-3 Ligand, respectively. The mouse and human Flt-3 Ligand proteins show cross-species activity (4, 5, 7). Flt-3 Ligand is also structurally related to M-CSF and SCF. Flt-3 Ligand is widely expressed in various mouse and human tissues. Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts (1, 8). Each 36 kDa chain of the Flt-3 Ligand dimer carries approximately 12 kDa of N- and O‑linked carbohydrates (8). Alternate splicing and proteolytic cleavage of the transmembrane form of the Flt-3 Ligand protein can generate a soluble 30 kDa fragment that includes the cytokine-like domain (4, 8). Alternate splicing of mouse Flt-3 Ligand also generates a membrane-associated isoform with a 57 aa substitution following the cytokine-like domain in the ECD of the Flt-3 Ligand protein (4, 5, 8, 9). Both transmembrane and soluble Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2 (3 - 6). Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation (2, 10). Additionally, Flt-3 Ligand synergizes with IL-3, GM‑CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells (4, 5, 7). Flt-3 Ligand also cooperates with IL‑2, IL-6, IL-7, and IL-15 to induce NK cell development and with IL-3, IL-7, and IL‑11 to induce terminal B cell maturation (1, 11). Animal studies also show that Flt-3 Ligand reduces the severity of experimentally induced allergic inflammation (12).

1. Wodnar-Filipowicz, A. (2003) News Physiol. Sci. 18:247.
2. Dong, J. et al. (2002) Cancer Biol. Ther. 1:486.
3. Gilliland, D.G. and J.D. Griffin (2002) Blood 100:1532.
4. Hannum, C. et al. (1994) Nature 368:643.
5. Lyman, S.D. et al. (1993) Cell 75:1157.
6. Savvides, S.N. et al. (2000) Nat. Struct. Biol. 7:486.
7. Lyman, S.D. et al. (1994) Blood 83:2795.
8. McClanahan, T. et al. (1996) Blood 88:3371.
9. Lyman, S.D. et al. (1995) Oncogene 10:149.
10. Diener, K.R. et al. (2008) Exp. Hematol. 36:51.
11. Farag, S.S. and M.A. Caligiuri (2006) Blood Rev. 20:123.
12. Edwan, J.H. et al. (2004) J. Immunol. 172:5016.

Publications for Flt-3 Ligand/FLT3L (427-FL)(96)

Publications using 427-FL

• Lau, L;Cariaga, TA;Chang, AB;Lane, JH;Purtha, WE;Rapaport, AS;Hu, R;Konno, H;Bulloch, DN;Rardin, MJ;Gibson, BW;Devoss, J;Ouyang, W;Manzanillo, PS; An essential role for TASL in mouse autoimmune pathogenesis and Toll-like receptor signaling Nature communications 2025-01-24 [PMID: 39856038] (Bioassay, Mouse)
• Gamble, N;Bradu, A;Caldwell, JA;McKeever, J;Bolonduro, O;Ermis, E;Kaiser, C;Kim, Y;Parks, B;Klemm, S;Greenleaf, WJ;Crabtree, GR;Koh, AS; PU.1 and BCL11B sequentially cooperate with RUNX1 to anchor mSWI/SNF to poise the T cell effector landscape Nature immunology 2024-04-17 [PMID: 38632339] (Bioassay, Mouse)
• Pv, A;Mehatre, SH;Verfaillie, CM;Alam, MT;Khurana, S; Glycolytic state of aortic endothelium favors hematopoietic transition during the emergence of definitive hematopoiesis Science advances 2024-02-16 [PMID: 38363844] (Bioassay, Mouse)
• Minutti, CM;Piot, C;Pereira da Costa, M;Chakravarty, P;Rogers, N;Huerga Encabo, H;Cardoso, A;Loong, J;Bessou, G;Mionnet, C;Langhorne, J;Bonnet, D;Dalod, M;Tomasello, E;Reis E Sousa, C; Distinct ontogenetic lineages dictate cDC2 heterogeneity Nature immunology 2024-02-13 [PMID: 38351322] (Bioassay, Mouse)
• Henry, CM;Castellanos, CA;Buck, MD;Giampazolias, E;Frederico, B;Cardoso, A;Rogers, NC;Schulz, O;Lee, S;Canton, J;Faull, P;Snijders, AP;Mohapatra, B;Band, H;Reis E Sousa, C; SYK ubiquitination by CBL E3 ligases restrains cross-presentation of dead cell-associated antigens by type 1 dendritic cells Cell reports 2023-11-28 [PMID: 38019655] (Bioassay, Mouse)
• Nicolao, MC;Rodrigues, CR;Coccimiglio, MB;Ledo, C;Docena, GH;Cumino, AC; Characterization of protein cargo of Echinococcus granulosus extracellular vesicles in drug response and its influence on immune response Parasites & vectors 2023-07-29 [PMID: 37516852] (Bioassay, Mouse)
• LR Palam, B Ramdas, KM Pickerell, SK Pasupuleti, R Kanumuri, A Cesarano, M Szymanski, BM Selman, UP Davé, G Sandusky, F Perna, S Paczesny, R Kapur Loss of Dnmt3a impairs hematopoietic homeostasis and myeloid cell skewing via the PI3Kinase pathway JCI Insight, 2023-05-08;0(0):. 2023-05-08 [PMID: 36976647] (Bioassay, Mouse)
• X Cui, C Zhang, F Wang, X Zhao, S Wang, J Liu, D He, C Wang, FC Yang, S Tong, Y Liang Latexin regulates sex dimorphism in hematopoiesis via gender-specific differential expression of microRNA 98-3p and thrombospondin 1 Cell Reports, 2023-03-16;42(3):112274. 2023-03-16 [PMID: 36933218] (Bioassay, Mouse)
• J Liu, Y Cheng, X Zhang, Y Chen, H Zhu, K Chen, S Liu, Z Li, X Cao Glycosyltransferase Extl1 promotes CCR7-mediated dendritic cell migration to restrain infection and autoimmunity Cell Reports, 2023-01-18;42(1):111991. 2023-01-18 [PMID: 36656709] (Bioassay, Mouse)
• X Liu, N Sato, T Yabushita, J Li, Y Jia, M Tamura, S Asada, T Fujino, T Fukushima, T Yonezawa, Y Tanaka, T Fukuyama, A Tsuchiya, S Shikata, H Iwamura, C Kinouchi, K Komatsu, S Yamasaki, T Shibata, AT Sasaki, J Schibler, M Wunderlich, E O'Brien, B Mizukawa, JC Mulloy, Y Sugiura, H Takizawa, T Shibata, K Miyake, T Kitamura, S Goyama IMPDH inhibition activates TLR-VCAM1 pathway and suppresses the development of MLL-fusion leukemia Embo Molecular Medicine, 2022-12-01;0(0):e15631. 2022-12-01 [PMID: 36453131] (Cell Culture, Human)
• M Uslu, E Albayrak, F Kocaba? Competitive inhibition of IL-2/IL-2R has a dual effect on HSC ex vivo expansion and IL-2R (CD25) content International immunopharmacology, 2022-07-11;110(0):109035. 2022-07-11 [PMID: 35834953] (Bioassay, Mouse)
• Y Hayashi, KC Kawabata, Y Tanaka, Y Uehara, Y Mabuchi, K Murakami, A Nishiyama, S Kiryu, Y Yoshioka, Y Ota, T Sugiyama, K Mikami, M Tamura, T Fukushima, S Asada, R Takeda, Y Kunisaki, T Fukuyama, K Yokoyama, T Uchida, M Hagihara, N Ohno, K Usuki, A Tojo, Y Katayama, S Goyama, F Arai, T Tamura, T Nagasawa, T Ochiya, D Inoue, T Kitamura MDS cells impair osteolineage differentiation of MSCs via extracellular vesicles to suppress normal hematopoiesis Cell Reports, 2022-05-10;39(6):110805. 2022-05-10 [PMID: 35545056] (Bioassay, Mouse)
• J Jeong, I Jung, JH Kim, S Jeon, DY Hyeon, H Min, B Kang, J Nah, D Hwang, SJ Um, M Ko, RH Seong BAP1 shapes the bone marrow niche for lymphopoiesis by fine-tuning epigenetic profiles in endosteal mesenchymal stromal cells Cell Death and Differentiation, 2022-04-26;0(0):. 2022-04-26 [PMID: 35473985] (Bioassay, Mouse)
• K Yamamoto, S Goyama, S Asada, T Fujino, T Yonezawa, N Sato, R Takeda, A Tsuchiya, T Fukuyama, Y Tanaka, A Yokoyama, H Toya, A Kon, Y Nannya, R Onoguchi-M, S Nakagawa, T Hirose, S Ogawa, N Akimitsu, T Kitamura A histone modifier, ASXL1, interacts with NONO and is involved in paraspeckle formation in hematopoietic cells Cell Reports, 2021-08-24;36(8):109576. 2021-08-24 [PMID: 34433054] (Bioassay, Mouse)
• N Ohashi, T Terashima, M Katagi, Y Nakae, J Okano, Y Suzuki, H Kojima GLT1 gene delivery based on bone marrow-derived cells ameliorates motor function and survival in a mouse model of ALS Scientific Reports, 2021-06-17;11(1):12803. 2021-06-17 [PMID: 34140581] (Cell Culture, Mouse)
• F Herbst, TJL Lang, ESP Eckert, P Wünsche, AA Wurm, T Kindinger, K Laaber, S Hemmati, A Hotz-Wagen, O Zavidij, A Paruzynski, J Lu, C von Kalle, T Zenz, C Klein, M Schmidt, CR Ball, H Glimm The balance between the intronic miR-342 and its host gene Evl determines hematopoietic cell fate decision Leukemia, 2021-05-21;0(0):. 2021-05-21 [PMID: 34021250] (Bioassay, Mouse)
• J Lu, S Li, X Li, W Zhao, X Duan, X Gu, J Xu, B Yu, LJ Sigal, Z Dong, L Xie, M Fang Declined miR-181a-5p expression is associated with impaired natural killer cell development and function with aging Aging Cell, 2021-03-29;0(0):e13353. 2021-03-29 [PMID: 33780118] (Bioassay, Mouse)
• V Tereshchen, A Bulygin, R Zavodskii, A Maksyutov, V Kurilin, M Fisher, N Semenyuk, S Aladev, S Sennikov The murine DCs transfected with DNA-plasmid encoding CCR9 demonstrate the increased migration to CCL25 and thymic cells in vitro and to the thymus in vivo Cytokine, 2021-02-26;142(0):155473. 2021-02-26 [PMID: 33647585] (Bioassay, Mouse)
• SP Fahl, AV Contreras, A Verma, X Qiu, C Harly, F Radtke, JC Zúñiga-Pfl, C Murre, HH Xue, JM Sen, DL Wiest The E protein-TCF1 axis controls &amp;gamma&amp;delta T�cell development and effector fate Cell Reports, 2021-02-02;34(5):108716. 2021-02-02 [PMID: 33535043] (Bioassay, Mouse)
• SJ Gurczynski, NL Pereira, SM Hrycaj, C Wilke, RL Zemans, BB Moore Stem cell transplantation uncovers TDO-AHR regulation of lung dendritic cells in herpesvirus-induced pathology JCI Insight, 2021-01-25;6(2):. 2021-01-25 [PMID: 33491663] (Bioassay, Mouse)
• NE Papaioanno, N Salei, S Rambichler, K Ravi, J Popovic, V Küntzel, CHK Lehmann, R Fiancette, J Salvermose, DW Gajdasik, R Mettler, D Messerer, J Carrelha, C Ohnmacht, D Haller, R Stumm, T Straub, SEW Jacobsen, C Schulz, DR Withers, G Schotta, D Dudziak, BU Schraml Environmental signals rather than layered ontogeny imprint the function of type 2 conventional dendritic cells in young and adult mice Nature Communications, 2021-01-19;12(1):464. 2021-01-19 [PMID: 33469015] (Bioassay, Mouse)
• Y Hong, N Singh, S Bamopoulos, E Gjerga, LK Schmalbroc, K Balabanian, M Schick, U Keller, M Wirth Authentication of Primary Murine Cell Lines by a Microfluidics-Based Lab-On-Chip System Biomedicines, 2020-12-09;8(12):. 2020-12-09 [PMID: 33317212] (Bioassay, Mouse)
• M Girotra, V Trachsel, A Roch, MP Lutolf In Vivo Pre-Instructed HSCs Robustly Execute Asymmetric Cell Divisions In Vitro Int J Mol Sci, 2020-11-03;21(21):. 2020-11-03 [PMID: 33153113] (Cell Culture, Mouse)
• M Wada, Y Shimada, S Iizuka, N Ishii, H Hiraki, T Tachibana, K Maeda, M Saito, S Arakawa, T Ishimoto, T Nakano, H Ida, T Ohashi, H Kobayashi Ex�Vivo Gene Therapy Treats Bone Complications of Mucopolysaccharidosis Type II Mouse Models through Bone Remodeling Reactivation Mol Ther Methods Clin Dev, 2020-09-20;19(0):261-274. 2020-09-20 [PMID: 33102618] (Bioassay, Mouse)
• NV Bogert, J Furkel, S Din, I Braren, V Eckstein, JA Müller, L Uhlmann, HA Katus, MH Konstandin A novel approach to genetic engineering of T-cell subsets by hematopoietic stem cell infection with a bicistronic lentivirus Sci Rep, 2020-08-13;10(1):13740. 2020-08-13 [PMID: 32792615] (Bioassay, Mouse)
• L Garcia-Per, M van Eggerm, L van Roon, SA Vloemans, M Cordes, A Schambach, M Rothe, D Berghuis, C Lagresle-P, M Cavazzana, F Zhang, AJ Thrasher, D Salvatori, P Meij, A Villa, JJM Van Dongen, JJ Zwaginga, M van der Bu, HB Gaspar, A Lankester, FJT Staal, K Pike-Overz Successful Preclinical Development of Gene Therapy for Recombinase-Activating Gene-1-Deficient SCID Mol Ther Methods Clin Dev, 2020-03-31;17(0):666-682. 2020-03-31 [PMID: 32322605] (Bioassay, Mouse)
• Y Zhao, J Wang, Z Min, L Peng, S Qi, W Lin I-alowCD11bhigh DC Regulates the Immune Response in the Eyes of Experimental Autoimmune Uveitis Mediators Inflamm., 2020-03-10;2020(0):6947482. 2020-03-10 [PMID: 32256194] (Bioassay, Mouse)
• JM Bertho, D Kereselidz, L Manens, C Culeux, V Magneron, J Surette, M Blimkie, L Bertrand, H Wyatt, M Souidi, I Dublineau, N Priest, JR Jourdain Hto, Tritiated Amino Acid Exposure and External Exposure Induce Differential Effects on Hematopoiesis and Iron Metabolism Sci Rep, 2019-12-27;9(1):19919. 2019-12-27 [PMID: 31882739] (Multiplex Assay, Mouse)
• ELY Chen, PK Thompson, JC Zúñiga-Pfl RBPJ-dependent Notch signaling initiates the T cell program in a subset of thymus-seeding progenitors Nat. Immunol., 2019-10-21;0(0):. 2019-10-21 [PMID: 31636466] (Cell Culture, Mouse)
• Z Gong, B Shang, Y Chu, X Chen, Q Li, K Liu, Y Chen, Y Huang, Y Han, Q Shang, Z Zheng, L Song, Y Li, R Liu, C Xu, X Zhang, B Liu, L Wang, C Shao, Y Wang, Y Shi Fibrotic liver microenvironment promotes Dll4 and SDF-1-dependent T-cell lineage development Cell Death Dis, 2019-06-05;10(6):440. 2019-06-05 [PMID: 31165736] (Bioassay, Mouse)
• P Huang, X Li, Y Meng, B Yuan, T Liu, M Jiao, X Wang, Y Liu, H Yin Interleukin-33 regulates hematopoietic stem cell regeneration after radiation injury Stem Cell Res Ther, 2019-04-18;10(1):123. 2019-04-18 [PMID: 30999922] (Bioassay, Mouse)
• N Vannini, V Campos, M Girotra, V Trachsel, S Rojas-Sutt, J Tratwal, S Ragusa, E Stefanidis, D Ryu, PY Rainer, G Nikitin, S Giger, TY Li, A Semilietof, A Oggier, Y Yersin, L Tauzin, E Pirinen, WC Cheng, J Ratajczak, C Canto, M Ehrbar, F Sizzano, TV Petrova, D Vanhecke, L Zhang, P Romero, A Nahimana, S Cherix, MA Duchosal, PC Ho, B Deplancke, G Coukos, J Auwerx, MP Lutolf, O Naveiras The NAD-Booster Nicotinamide Riboside Potently Stimulates Hematopoiesis through Increased Mitochondrial Clearance Cell Stem Cell, 2019-03-07;24(3):405-418.e7. 2019-03-07 [PMID: 30849366] (Bioassay, Mouse)
• S Goossens, J Wang, C Tremblay, J De Medts, S T'Sas, T Nguyen, J Saw, K Haigh, DJ Curtis, P Van Vlierb, G Berx, T Taghon, JJ Haigh ZEB2 and LMO2 drive immature T-cell lymphoblastic leukemia via distinct oncogenic mechanisms Haematologica, 2019-01-24;0(0):. 2019-01-24 [PMID: 30679322] (Bioassay, Mouse)
• F Ribezzo, IAM Snoeren, S Ziegler, J Stoelben, PA Olofsen, A Henic, MV Ferreira, S Chen, USA Stalmann, G Buesche, RM Hoogenboez, R Kramann, U Platzbecke, MHGP Raaijmaker, BL Ebert, RK Schneider Rps14, Csnk1a1 and miRNA145/miRNA146a deficiency cooperate in the clinical phenotype and activation of the innate immune system in the 5q- syndrome Leukemia, 2019-01-16;0(0):. 2019-01-16 [PMID: 30651631] (Bioassay, Mouse)
• F Boscolo Se, D Fox, A Sacco Muscle Stem Cells Give Rise to Rhabdomyosarcomas in a Severe Mouse Model of Duchenne Muscular Dystrophy Cell Rep, 2019-01-15;26(3):689-701.e6. 2019-01-15 [PMID: 30650360] (Bioassay, Mouse)
• LM Hillyer, B Woodward Acutely malnourished weanling mice administered Flt3 ligand can support a cell-mediated inflammatory response Cytokine, 2018-07-07;113(0):39-49. 2018-07-07 [PMID: 30539781] (ELISA (Standard), Western Blot, Human)
• M Inoue, K Okamoto, A Terashima, T Nitta, R Muro, T Negishi-Ko, T Kitamura, T Nakashima, H Takayanagi Arginine methylation controls the strength of ?c-family cytokine signaling in T cell maintenance Nat. Immunol., 2018-10-15;19(11):1265-1276. 2018-10-15 [PMID: 30323341] (Bioassay, Mouse)
• SK Martin, S Fitter, N El Khawank, RH Grose, CR Walkley, LE Purton, MA Ruegg, MN Hall, S Gronthos, ACW Zannettino mTORC1 plays an important role in osteoblastic regulation of B-lymphopoiesis Sci Rep, 2018-09-28;8(1):14501. 2018-09-28 [PMID: 30266921] (Bioassay, Mouse)
• R Gonzalez-Q, A Nguyen, DH Kono, MBA Oldstone, AN Theofilopo, R Baccala Lupus acceleration by a MAVS-activating RNA virus requires endosomal TLR signaling and host genetic predisposition PLoS ONE, 2018-09-10;13(9):e0203118. 2018-09-10 [PMID: 30199535] (Bioassay, Mouse)
• YW Lin, LC Wang, CK Lee, SH Chen Flt3 ligand treatment reduces enterovirus A71 lethality in mice with enhanced B cell responses Sci Rep, 2018-08-15;8(1):12184. 2018-08-15 [PMID: 30111869] (In Vivo, Mouse)
• SH Kim, SW Lee, HJ Park, SH Lee, WK Im, YD Kim, KH Kim, SJ Park, S Hong, SH Jeon Anti-cancer activity of Angelica gigas by increasing immune response and stimulating natural killer and natural killer T cells BMC Complement Altern Med, 2018-07-18;18(1):218. 2018-07-18 [PMID: 30021579] (Bioassay, Mouse)
• P Wünsche, ESP Eckert, T Holland-Le, A Paruzynski, A Hotz-Wagen, R Fronza, T Rath, I Gil-Farina, M Schmidt, C von Kalle, C Klein, CR Ball, F Herbst, H Glimm Mapping Active Gene-Regulatory Regions in Human Repopulating Long-Term HSCs Cell Stem Cell, 2018-07-05;23(1):132-146.e9. 2018-07-05 [PMID: 29979988] (Bioassay, Mouse)
• SK Tasian, JA Casas, D Posocco, S Gandre-Bab, AL Gagne, G Liang, ML Loh, MJ Weiss, DL French, ST Chou Mutation-specific signaling profiles and kinase inhibitor sensitivities of juvenile myelomonocytic leukemia revealed by induced pluripotent stem cells Leukemia, 2018-06-08;0(0):. 2018-06-08 [PMID: 29884903] (Bioassay, Mouse)
• JP Sasine, HA Himburg, CM Termini, M Roos, E Tran, L Zhao, J Kan, M Li, Y Zhang, SC de Barros, DS Rao, CM Counter, JP Chute Wild-type Kras expands and exhausts hematopoietic stem cells JCI Insight, 2018-06-07;3(11):. 2018-06-07 [PMID: 29875320] (Bioassay, Mouse)
• GH Nam, EJ Lee, YK Kim, Y Hong, Y Choi, MJ Ryu, J Woo, Y Cho, DJ Ahn, Y Yang, IC Kwon, SY Park, IS Kim Combined Rho-kinase inhibition and immunogenic cell death triggers and propagates immunity against cancer Nat Commun, 2018-06-04;9(1):2165. 2018-06-04 [PMID: 29867097] (Bioassay, Mouse)
• LC Cheung, J Tickner, AM Hughes, P Skut, M Howlett, B Foley, J Oommen, JE Wells, B He, S Singh, GA Chua, J Ford, CG Mullighan, RS Kotecha, UR Kees New therapeutic opportunities from dissecting the pre-B leukemia bone marrow microenvironment Leukemia, 2018-05-08;0(0):. 2018-05-08 [PMID: 29740160] (Bioassay, Mouse)
• HT Fang, CA El Farran, QR Xing, LF Zhang, H Li, B Lim, YH Loh Global H3.3 dynamic deposition defines its bimodal role in cell fate transition Nat Commun, 2018-04-18;9(1):1537. 2018-04-18 [PMID: 29670118] (Bioassay, Mouse)
• M Kanayama, Y Kato, T Tsuji, Y Konoeda, A Hashimoto, O Kanauchi, T Fujii, D Fujiwara Enhancement of immunomodulative effect of lactic acid bacteria on plasmacytoid dendritic cells with sucrose palmitate Sci Rep, 2018-02-16;8(1):3147. 2018-02-16 [PMID: 29453437] (Bioassay, Mouse)
• Y Xiao, J Palomero, J Grabowska, L Wang, I de Rink, L van Helver, J Borst Macrophages and osteoclasts stem from a bipotent progenitor downstream of a macrophage/osteoclast/dendritic cell progenitor Blood Adv, 2017-10-13;1(23):1993-2006. 2017-10-13 [PMID: 29296846] (Differentiation, Differentiation, Mouse)
• J Huang, A Khan, BC Au, DL Barber, L López-Vásq, NL Prokopishy, M Boutin, M Rothe, JW Rip, M Abaoui, MS Nagree, S Dworski, A Schambach, A Keating, ML West, J Klassen, PV Turner, S Sirrs, CA Rupar, C Auray-Blai, R Foley, JA Medin Lentivector Iterations and Pre-Clinical Scale-Up/Toxicity Testing: Targeting Mobilized CD34(+) Cells for Correction of Fabry Disease Mol Ther Methods Clin Dev, 2017-05-12;5(0):241-258. 2017-05-12 [PMID: 28603745] (Bioassay, Mouse)
• MJ Smith, DK Reichenbac, SL Parker, MJ Riddle, J Mitchell, KC Osum, M Mohtashami, HE Stefanski, BT Fife, A Bhandoola, KA Hogquist, GA Holländer, JC Zúñiga-Pfl, J Tolar, BR Blazar T cell progenitor therapy-facilitated thymopoiesis depends upon thymic input and continued thymic microenvironment interaction JCI Insight, 2017-05-18;2(10):. 2017-05-18 [PMID: 28515359] (Bioassay, Mouse)
• S Ma, X Wan, Z Deng, L Shi, C Hao, Z Zhou, C Zhou, Y Fang, J Liu, J Yang, X Chen, T Li, A Zang, S Yin, B Li, J Plumas, L Chaperot, X Zhang, G Xu, L Jiang, N Shen, S Xiong, X Gao, Y Zhang, H Xiao Epigenetic regulator CXXC5 recruits DNA demethylase Tet2 to regulate TLR7/9-elicited IFN response in pDCs J. Exp. Med., 2017-04-17;0(0):. 2017-04-17 [PMID: 28416650] (Bioassay, Mouse)
• S Shukla, MA Langley, J Singh, JM Edgar, M Mohtashami, JC Zúñiga-Pfl, PW Zandstra Progenitor T-cell differentiation from hematopoietic stem cells using Delta-like-4 and VCAM-1 Nat. Methods, 2017-04-10;14(5):531-538. 2017-04-10 [PMID: 28394335] (Bioassay, Mouse)
• S Kumar, R Sunagar, G Pham, BJ Franz, SJ Rosa, KR Hazlett, EJ Gosselin Differential Cultivation of Francisella tularensis Induces Changes in the Immune Response to and Protective Efficacy of Whole Cell-Based Inactivated Vaccines Front Immunol, 2017-01-10;7(0):677. 2017-01-10 [PMID: 28119692] (Bioassay, Mouse)
• Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms Nat. Med, 2016-12-05;0(0):. 2016-12-05 [PMID: 27918563] (Bioassay, Mouse)
• The non-canonical Wnt receptor Ryk regulates hematopoietic stem cell repopulation in part by controlling proliferation and apoptosis Cell Death Dis, 2016-11-24;7(11):e2479. 2016-11-24 [PMID: 27882948] (Bioassay, Mouse)
• Reprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors Nat Commun, 2016-11-21;7(0):13396. 2016-11-21 [PMID: 27869129] (Bioassay, Mouse)
• L Zhao, RL Frock, Z Du, J Hu, L Chen, MS Krangel, FW Alt Orientation-specific RAG activity in chromosomal loop domains contributes to Tcrd V(D)J recombination during T cell development J. Exp. Med, 2016-08-15;213(9):1921-36. 2016-08-15 [PMID: 27526713] (Bioassay, Mouse)
• Hepatitis C Virus Stimulates Murine CD8?-Like Dendritic Cells to Produce Type I Interferon in a TRIF-Dependent Manner PLoS Pathog, 2016-07-06;12(7):e1005736. 2016-07-06 [PMID: 27385030] (Bioassay, Mouse)
• Ahmed S Elshikha Alpha 1 Antitrypsin Inhibits Dendritic Cell Activation and Attenuates Nephritis in a Mouse Model of Lupus PLoS ONE, 2016-05-27;11(5):e0156583. 2016-05-27 [PMID: 27232337] (Bioassay, Mouse)
• Gene Therapy Induces Antigen-Specific Tolerance in Experimental Collagen-Induced Arthritis PLoS ONE, 2016-05-09;11(5):e0154630. 2016-05-09 [PMID: 27159398] (Bioassay, Mouse)
• Modulation of splicing catalysis for therapeutic targeting of leukemia with mutations in genes encoding spliceosomal proteins Nat. Med., 2016-05-02;22(6):672-8. 2016-05-02 [PMID: 27135740] (Bioassay, Mouse)
• Niedzielska M, Raffi F, Tel J, Muench S, Jozefowski K, Alati N, Lahl K, Mages J, Billmeier U, Schiemann M, Appelt U, Wirtz S, Sparwasser T, Hochrein H, Figdor C, Keyse S, Lang R Selective Expression of the MAPK Phosphatase Dusp9/MKP-4 in Mouse Plasmacytoid Dendritic Cells and Regulation of IFN-beta Production. J Immunol, 2015-07-13;195(4):1753-62. 2015-07-13 [PMID: 26170386] (In Vivo, Mouse)
• Lee S, Park H, Jeon S, Lee C, Seong R, Park S, Hong S Ubiquitous Over-Expression of Chromatin Remodeling Factor SRG3 Ameliorates the T Cell-Mediated Exacerbation of EAE by Modulating the Phenotypes of both Dendritic Cells and Macrophages. PLoS ONE, 2015-07-06;10(7):e0132329. 2015-07-06 [PMID: 26147219] (Bioassay, Mouse)
• Flores M, Chew C, Tyan K, Huang W, Salem A, Clynes R FcgammaRIIB prevents inflammatory type I IFN production from plasmacytoid dendritic cells during a viral memory response. J Immunol, 2015-03-27;194(9):4240-50. 2015-03-27 [PMID: 25821224] (Bioassay, Mouse)
• Rommler F, Hammel M, Waldhuber A, Muller T, Jurk M, Uhlmann E, Wagner H, Vollmer J, Miethke T Guanine-modified inhibitory oligonucleotides efficiently impair TLR7- and TLR9-mediated immune responses of human immune cells. PLoS ONE, 2015-02-19;10(2):e0116703. 2015-02-19 [PMID: 25695778] (Bioassay, Mouse)
• Scheuplein V, Seifried J, Malczyk A, Miller L, Hocker L, Vergara-Alert J, Dolnik O, Zielecki F, Becker B, Spreitzer I, Konig R, Becker S, Waibler Z, Muhlebach M High secretion of interferons by human plasmacytoid dendritic cells upon recognition of Middle East respiratory syndrome coronavirus. J Virol, 2015-01-21;89(7):3859-69. 2015-01-21 [PMID: 25609809] (Bioassay, Mouse)
• Lignin-rich enzyme lignin (LREL), a cellulase-treated lignin-carbohydrate derived from plants, activates myeloid dendritic cells via Toll-like receptor 4 (TLR4). J Biol Chem, 2014-12-29;290(0):4410-21. 2014-12-29 [PMID: 25548274] (Bioassay, Mouse)
• Martinez-Lopez M, Iborra S, Conde-Garrosa R, Sancho D Batf3-dependent CD103+ dendritic cells are major producers of IL-12 that drive local Th1 immunity against Leishmania major infection in mice. Eur J Immunol, 2014-11-28;45(1):119-29. 2014-11-28 [PMID: 25312824] (Bioassay, Mouse)
• Boettcher S, Gerosa R, Radpour R, Bauer J, Ampenberger F, Heikenwalder M, Kopf M, Manz M Endothelial cells translate pathogen signals into G-CSF-driven emergency granulopoiesis. Blood, 2014-07-02;124(9):1393-403. 2014-07-02 [PMID: 24990886] (Bioassay, Mouse)
• Patnode M, Bando J, Krummel M, Locksley R, Rosen S Leukotriene B4 amplifies eosinophil accumulation in response to nematodes. J Exp Med, 2014-06-02;211(7):1281-8. 2014-06-02 [PMID: 24889202] (Bioassay, Mouse)
• Dai P, Wang W, Cao H, Avogadri F, Dai L, Drexler I, Joyce J, Li X, Chen Z, Merghoub T, Shuman S, Deng L Modified vaccinia virus Ankara triggers type I IFN production in murine conventional dendritic cells via a cGAS/STING-mediated cytosolic DNA-sensing pathway. PLoS Pathog, 2014-04-17;10(4):e1003989. 2014-04-17 [PMID: 24743339] (Bioassay, Mouse)
• Treanor L, Zhou S, Janke L, Churchman M, Ma Z, Lu T, Chen S, Mullighan C, Sorrentino B Interleukin-7 receptor mutants initiate early T cell precursor leukemia in murine thymocyte progenitors with multipotent potential. J Exp Med, 2014-03-31;211(4):701-13. 2014-03-31 [PMID: 24687960] (Bioassay, Mouse)
• Wallington-Beddoe C, Powell J, Tong D, Pitson S, Bradstock K, Bendall L Sphingosine kinase 2 promotes acute lymphoblastic leukemia by enhancing MYC expression. Cancer Res, 2014-03-31;74(10):2803-15. 2014-03-31 [PMID: 24686171] (Bioassay, Mouse)
• Krutikov K, Zheng Y, Chesney A, Huang X, Vaags A, Evdokimova V, Hough M, Chen E Ectopic TLX1 expression accelerates malignancies in mice deficient in DNA-PK. PLoS ONE, 2014-02-26;9(2):e89649. 2014-02-26 [PMID: 24586935] (Bioassay, Mouse)
• Coffey F, Lee S, Buus T, Lauritsen J, Wong G, Joachims M, Thompson L, Zuniga-Pflucker J, Kappes D, Wiest D The TCR ligand-inducible expression of CD73 marks gammadelta lineage commitment and a metastable intermediate in effector specification. J Exp Med, 2014-02-03;211(2):329-43. 2014-02-03 [PMID: 24493796] (Bioassay, Mouse)
• Yamamizu K, Piao Y, Sharov A, Zsiros V, Yu H, Nakazawa K, Schlessinger D, Ko M Identification of transcription factors for lineage-specific ESC differentiation. Stem Cell Reports, 2013-11-27;1(6):545-59. 2013-11-27 [PMID: 24371809] (Bioassay, Mouse)
• Narendra S, Chalise J, Hook N, Magnusson M Dendritic cells activated by double-stranded RNA induce arthritis via autocrine type I IFN signaling. J Leukoc Biol, 2013-12-04;95(4):661-6. 2013-12-04 [PMID: 24304616] (Bioassay, Mouse)
• Gao , Xin, Johnson , Kirby D, Chang , Yuan-I, Boyer , Meghan E, Dewey , Colin N, Zhang , Jing, Bresnick , Emery H Gata2 cis-element is required for hematopoietic stem cell generation in the mammalian embryo. J Exp Med, 2013-12-02;210(13):2833-42. 2013-12-02 [PMID: 24297994] (Bioassay, Mouse)
• Pereira C, Chang B, Qiu J, Niu X, Papatsenko D, Hendry C, Clark N, Nomura-Kitabayashi A, Kovacic J, Ma'ayan A, Schaniel C, Lemischka I, Moore K Induction of a hemogenic program in mouse fibroblasts. Cell Stem Cell, 2013-06-13;13(2):205-18. 2013-06-13 [PMID: 23770078] (Bioassay, Mouse)
• Manley , Nathan C, Caso , Javier R, Works , Melissa, Cutler , Andrew B, Zemlyak , Ilona, Sun , Guohua, Munhoz , Carolina, Chang , Sydney, Sorrells , Shawn F, Ermini , Florian, Decker , Johannes, Bertrand , Anthony, Dinkel , Klaus M, Steinberg , Gary K, Sapolsky , Robert M Derivation of injury-responsive dendritic cells for acute brain targeting and therapeutic protein delivery in the stroke-injured rat. PLoS ONE, 2013-04-16;8(4):e61789. 2013-04-16 [PMID: 23613937] (Bioassay, Rat)
• Kim D, Niewiesk S Synergistic induction of interferon alpha through TLR-3 and TLR-9 agonists identifies CD21 as interferon alpha receptor for the B cell response. PLoS Pathog, 2013-03-14;9(3):e1003233. 2013-03-14 [PMID: 23516365] (Bioassay, Cotton Rat)
• Gatto D, Wood K, Caminschi I, Murphy-Durland D, Schofield P, Christ D, Karupiah G, Brink R The chemotactic receptor EBI2 regulates the homeostasis, localization and immunological function of splenic dendritic cells. Nat Immunol, 2013-03-17;14(5):446-53. 2013-03-17 [PMID: 23502855] (Bioassay, Mouse)
• Smith , Christop, Berry , Donna M, McGlade , C Jane The E3 ubiquitin ligases RNF126 and Rabring7 regulate endosomal sorting of the epidermal growth factor receptor. J Cell Sci, 2013-02-15;126(0):1366-80. 2013-02-15 [PMID: 23418353] (Bioassay, Mouse)
• Iborra S, Izquierdo HM, Martinez-Lopez M, Blanco-Menendez N, Reis E Sousa C, Sancho D The DC receptor DNGR-1 mediates cross-priming of CTLs during vaccinia virus infection in mice. J. Clin. Invest., 2012-04-16;122(5):1628-43. 2012-04-16 [PMID: 22505455] (Bioassay, Mouse)
• Shinohara ML, Kim JH, Garcia VA, Cantor H Engagement of the type I interferon receptor on dendritic cells inhibits T helper 17 cell development: role of intracellular osteopontin. Immunity, 2008-07-18;29(1):68-78. 2008-07-18 [PMID: 18619869] (Bioassay, Mouse)
• Zelante T, De Luca A, Bonifazi P, Montagnoli C, Bozza S, Moretti S, Belladonna ML, Vacca C, Conte C, Mosci P, Bistoni F, Puccetti P, Kastelein RA, Kopf M, Romani L IL-23 and the Th17 pathway promote inflammation and impair antifungal immune resistance. Eur. J. Immunol., 2007-10-01;37(10):2695-706. 2007-10-01 [PMID: 17899546] (Bioassay, Mouse)
• Rajesh</LastName><ForeNam D</Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells. Stem Cells, 2007-02-01;25(2):490-9. 2007-02-01 [PMID: 17284653] (Bioassay, Human, Primate - Macaca mulatta (Rhesus Macaque))
• Kared H, Adle-Biassette H, Fois E, Masson A, Bach JF, Chatenoud L, Schneider E, Zavala F Jagged2-expressing hematopoietic progenitors promote regulatory T cell expansion in the periphery through notch signaling. Immunity, 2006-11-02;25(5):823-34. 2006-11-02 [PMID: 17081781] (In Vivo, Mouse)
• Braun BS, Archard JA, Van Ziffle JA, Tuveson DA, Jacks TE, Shannon K Somatic activation of a conditional KrasG12D allele causes ineffective erythropoiesis in vivo. Blood, 2006-05-23;108(6):2041-4. 2006-05-23 [PMID: 16720837] (Bioassay, Mouse)
• Yoshihiro Ueda, Motonari Kondo, Garnett Kelsoe Inflammation and the reciprocal production of granulocytes and lymphocytes in bone marrow The Journal of Experimental Medicine 2005-06-06 [PMID: 15939792] (Bioassay, Mouse)
• Azhipa O, Kimizuka K, Nakao A, Toyokawa H, Okuda T, Neto JS, Alber SM, Kaizu T, Thomson AW, Demetris AJ, Murase N Comparative analysis of the fate of donor dendritic cells and B cells and their influence on alloreactive T cell responses under tacrolimus immunosuppression. Clin. Immunol., 2005-02-01;114(2):199-209. 2005-02-01 [PMID: 15639654] (Bioassay, Rat)
• Miller MJ, Hejazi AS, Wei SH, Cahalan MD, Parker I T cell repertoire scanning is promoted by dynamic dendritic cell behavior and random T cell motility in the lymph node. Proc. Natl. Acad. Sci. U.S.A., 2004-01-13;101(4):998-1003. 2004-01-13 [PMID: 14722354] (In Vivo, Mouse)
• Asselin-Paturel C, Brizard G, Pin JJ, Briere F, Trinchieri G Mouse strain differences in plasmacytoid dendritic cell frequency and function revealed by a novel monoclonal antibody. J. Immunol., 2003-12-15;171(12):6466-77. 2003-12-15 [PMID: 14662846] (Bioassay, Mouse)
• Blumenthal RD, Reising A, Leon E Modulation of marrow proliferation and chemosensitivity by tumor-produced cytokines from syngeneic pancreatic tumor lines. Clin. Cancer Res., 2002-05-01;8(5):1301-9. 2002-05-01 [PMID: 12006552] (Bioassay, Mouse)
• Shih CC, Hu MC, Hu J A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells. Blood, 2000-03-15;95(6):1957-66. 2000-03-15 [PMID: 10706861] (ELISA Developmet, Mouse)

Bioinformatics

• Gene Symbol: Flt3l
• Uniprot:
  • P49772.1()