Recombinant Mouse Flt-3 Ligand/FLT3L Protein

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1 μg/lane of Recombinant Mouse Flt-3 Ligand/FLT3L was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing multiple bands between 18-32 kDa. Multiple bands are due to variable ...read more
Recombinant Mouse Flt-3 Ligand/FLT3L (Catalog # 427-FL) stimulates cell proliferation in the BaF3 mouse pro-B cell line transfected with mouse Flt-3. The ED50 for this effect is 0.4-2.4 ng/mL.

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Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse Flt-3 Ligand/FLT3L Protein Summary

Additional Information
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Details of Functionality
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with mouse Flt-3. The ED50 for this effect is typically 0.4-2.4 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Flt-3 Ligand/FLT3L protein
Gly27-Arg188
Accession #
N-terminal Sequence
Gly27
Protein/Peptide Type
Recombinant Proteins
Gene
Flt3l
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
Multiple bands between 18-32 kDa, reducing conditions
Publications
Read Publications using
427-FL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Flt-3 Ligand/FLT3L Protein

  • FL
  • FLG3L
  • Flt3 ligand
  • Flt-3 Ligand
  • Flt3L
  • FLT3LG
  • fms-related tyrosine kinase 3 ligand
  • SL cytokine

Background

Flt-3 Ligand, also known as FLT3L, is an alpha-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages (1-3). Mature mouse Flt‑3 Ligand consists of a 161 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 22 aa cytoplasmic tail (4-6). Within the ECD, mouse Flt-3 Ligand shares 71% and 81% aa sequence identity with human and rat Flt-3 Ligand, respectively. The mouse and human Flt-3 Ligand proteins show cross-species activity (4, 5, 7). Flt-3 Ligand is also structurally related to M-CSF and SCF. Flt-3 Ligand is widely expressed in various mouse and human tissues. Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts (1, 8). Each 36 kDa chain of the Flt-3 Ligand dimer carries approximately 12 kDa of N- and O‑linked carbohydrates (8). Alternate splicing and proteolytic cleavage of the transmembrane form of the Flt-3 Ligand protein can generate a soluble 30 kDa fragment that includes the cytokine-like domain (4, 8). Alternate splicing of mouse Flt-3 Ligand also generates a membrane-associated isoform with a 57 aa substitution following the cytokine-like domain in the ECD of the Flt-3 Ligand protein (4, 5, 8, 9). Both transmembrane and soluble Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2 (3 - 6). Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation (2, 10). Additionally, Flt-3 Ligand synergizes with IL-3, GM‑CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells (4, 5, 7). Flt-3 Ligand also cooperates with IL‑2, IL-6, IL-7, and IL-15 to induce NK cell development and with IL-3, IL-7, and IL‑11 to induce terminal B cell maturation (1, 11). Animal studies also show that Flt-3 Ligand reduces the severity of experimentally induced allergic inflammation (12).
  1. Wodnar-Filipowicz, A. (2003) News Physiol. Sci. 18:247.
  2. Dong, J. et al. (2002) Cancer Biol. Ther. 1:486.
  3. Gilliland, D.G. and J.D. Griffin (2002) Blood 100:1532.
  4. Hannum, C. et al. (1994) Nature 368:643.
  5. Lyman, S.D. et al. (1993) Cell 75:1157.
  6. Savvides, S.N. et al. (2000) Nat. Struct. Biol. 7:486.
  7. Lyman, S.D. et al. (1994) Blood 83:2795.
  8. McClanahan, T. et al. (1996) Blood 88:3371.
  9. Lyman, S.D. et al. (1995) Oncogene 10:149.
  10. Diener, K.R. et al. (2008) Exp. Hematol. 36:51.
  11. Farag, S.S. and M.A. Caligiuri (2006) Blood Rev. 20:123.
  12. Edwan, J.H. et al. (2004) J. Immunol. 172:5016.

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Publications for Flt-3 Ligand/FLT3L (427-FL)(85)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Cotton Rat.

We have publications tested in 7 applications: Bioassay, Cell Culture, Differentiation, ELISA (Standard), ELISA Developmet, In Vivo, Multiplex Assay.


Filter By Application
Bioassay
(74)
Cell Culture
(3)
Differentiation
(1)
ELISA (Standard)
(1)
ELISA Developmet
(1)
In Vivo
(4)
Multiplex Assay
(1)
All Applications
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Human
(2)
Mouse
(80)
Rat
(2)
Cotton Rat
(1)
All Species
Showing Publications 1 - 10 of 85. Show All 85 Publications.
Publications using 427-FL Applications Species
Y Hayashi, KC Kawabata, Y Tanaka, Y Uehara, Y Mabuchi, K Murakami, A Nishiyama, S Kiryu, Y Yoshioka, Y Ota, T Sugiyama, K Mikami, M Tamura, T Fukushima, S Asada, R Takeda, Y Kunisaki, T Fukuyama, K Yokoyama, T Uchida, M Hagihara, N Ohno, K Usuki, A Tojo, Y Katayama, S Goyama, F Arai, T Tamura, T Nagasawa, T Ochiya, D Inoue, T Kitamura MDS cells impair osteolineage differentiation of MSCs via extracellular vesicles to suppress normal hematopoiesis Cell Reports, 2022;39(6):110805. 2022 [PMID: 35545056] (Bioassay, Mouse) Bioassay Mouse
J Jeong, I Jung, JH Kim, S Jeon, DY Hyeon, H Min, B Kang, J Nah, D Hwang, SJ Um, M Ko, RH Seong BAP1 shapes the bone marrow niche for lymphopoiesis by fine-tuning epigenetic profiles in endosteal mesenchymal stromal cells Cell Death and Differentiation, 2022;0(0):. 2022 [PMID: 35473985] (Bioassay, Mouse) Bioassay Mouse
K Yamamoto, S Goyama, S Asada, T Fujino, T Yonezawa, N Sato, R Takeda, A Tsuchiya, T Fukuyama, Y Tanaka, A Yokoyama, H Toya, A Kon, Y Nannya, R Onoguchi-M, S Nakagawa, T Hirose, S Ogawa, N Akimitsu, T Kitamura A histone modifier, ASXL1, interacts with NONO and is involved in paraspeckle formation in hematopoietic cells Cell Reports, 2021;36(8):109576. 2021 [PMID: 34433054] (Bioassay, Mouse) Bioassay Mouse
N Ohashi, T Terashima, M Katagi, Y Nakae, J Okano, Y Suzuki, H Kojima GLT1 gene delivery based on bone marrow-derived cells ameliorates motor function and survival in a mouse model of ALS Scientific Reports, 2021;11(1):12803. 2021 [PMID: 34140581] (Cell Culture, Mouse) Cell Culture Mouse
F Herbst, TJL Lang, ESP Eckert, P Wünsche, AA Wurm, T Kindinger, K Laaber, S Hemmati, A Hotz-Wagen, O Zavidij, A Paruzynski, J Lu, C von Kalle, T Zenz, C Klein, M Schmidt, CR Ball, H Glimm The balance between the intronic miR-342 and its host gene Evl determines hematopoietic cell fate decision Leukemia, 2021;0(0):. 2021 [PMID: 34021250] (Bioassay, Mouse) Bioassay Mouse
J Lu, S Li, X Li, W Zhao, X Duan, X Gu, J Xu, B Yu, LJ Sigal, Z Dong, L Xie, M Fang Declined miR-181a-5p expression is associated with impaired natural killer cell development and function with aging Aging Cell, 2021;0(0):e13353. 2021 [PMID: 33780118] (Bioassay, Mouse) Bioassay Mouse
V Tereshchen, A Bulygin, R Zavodskii, A Maksyutov, V Kurilin, M Fisher, N Semenyuk, S Aladev, S Sennikov The murine DCs transfected with DNA-plasmid encoding CCR9 demonstrate the increased migration to CCL25 and thymic cells in vitro and to the thymus in vivo Cytokine, 2021;142(0):155473. 2021 [PMID: 33647585] (Bioassay, Mouse) Bioassay Mouse
SP Fahl, AV Contreras, A Verma, X Qiu, C Harly, F Radtke, JC Zúñiga-Pfl, C Murre, HH Xue, JM Sen, DL Wiest The E protein-TCF1 axis controls &amp;gamma&amp;delta T�cell development and effector fate Cell Reports, 2021;34(5):108716. 2021 [PMID: 33535043] (Bioassay, Mouse) Bioassay Mouse
SJ Gurczynski, NL Pereira, SM Hrycaj, C Wilke, RL Zemans, BB Moore Stem cell transplantation uncovers TDO-AHR regulation of lung dendritic cells in herpesvirus-induced pathology JCI Insight, 2021;6(2):. 2021 [PMID: 33491663] (Bioassay, Mouse) Bioassay Mouse
NE Papaioanno, N Salei, S Rambichler, K Ravi, J Popovic, V Küntzel, CHK Lehmann, R Fiancette, J Salvermose, DW Gajdasik, R Mettler, D Messerer, J Carrelha, C Ohnmacht, D Haller, R Stumm, T Straub, SEW Jacobsen, C Schulz, DR Withers, G Schotta, D Dudziak, BU Schraml Environmental signals rather than layered ontogeny imprint the function of type 2 conventional dendritic cells in young and adult mice Nature Communications, 2021;12(1):464. 2021 [PMID: 33469015] (Bioassay, Mouse) Bioassay Mouse
Show All 85 Publications.

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Bioinformatics

Gene Symbol Flt3l
Uniprot