HAF109). A specific band was detected for Klotho at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse Klotho Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1819) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Klotho at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1819-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76511-0.025mg | |||
DY990). Goat Anti-Mouse Klotho Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1819) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Mouse Klotho protein was serially diluted 2-fold and captured by Rat Anti-Mouse Klotho Monoclonal Antibody (Catalog # MAB1819) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Klotho Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1819) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1819-SP | |||
5334-KL) induces proliferation in the BaF3 mouse pro-B cell line transfected with human FGF RIIIc in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Klotho (0.3 µg/mL) is neutralized (green line) by increasing concen-trations of Rat Anti-Human Klotho Monoclonal Antibody (Catalog # MAB5334). The ND50 is typically 0.15-0.6 µg/mL." alt="Recombinant Human Klotho (Catalog # 5334-KL) induces proliferation in the BaF3 mouse pro-B cell line transfected with human FGF RIIIc in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Klotho (0.3 µg/mL) is neutralized (green line) by increasing concen-trations of Rat Anti-Human Klotho Monoclonal Antibody (Catalog # MAB5334). The ND50 is typically 0.15-0.6 µg/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5334-SP | |||
| Carrier Free | MAB5334-500 | |||
CTS008) and counterstained with hematoxylin (blue). View our protocol for 
MAB1819) coated on a Clear Polystyrene Microplate (Catalog # 
![Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human KLOTHO recombinant protein per lane. Antibodies: KLOTHO NBP1-76511 (Lane 1: 1 ug/mL, Lane 2: 2 ug/mL and Lane 3: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Klotho-Antibody---BSA-Free-Western-Blot-NBP1-76511-img0010.jpg "Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human KLOTHO recombinant protein per lane. Antibodies: KLOTHO NBP1-76511 (Lane 1: 1 ug/mL, Lane 2: 2 ug/mL and Lane 3: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunohistochemistry: Klotho Antibody - BSA Free [NBP1-76511] - <strong>Figure 6 Immunohistochemistry Validation of Klotho in Rat Heart Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Klotho antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76511_rabbit-polyclonal-klotho-antibody-271120242013162.gif)
![Immunohistochemistry-Paraffin: Klotho Antibody - BSA Free [NBP1-76511] - Mouse heart tissue diluted at 2.5 ug/ml.](http://images.novusbio.com/fullsize/Klotho-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-76511-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Klotho Antibody - BSA Free [NBP1-76511] - Figure 5 Immunofluorescence Validation of Klotho in Mouse Heart TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart tissue labeling Klotho with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76511_rabbit-polyclonal-klotho-antibody-2711202420212384.jpg)
![Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Validation in Rat Kidney Tissue LysateLoading: 15ug of lysates per lane.Antibodies: KLOTHO 6107 (1ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Klotho-Antibody---BSA-Free-Western-Blot-NBP1-76511-img0007.jpg)
![Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human and Mouse Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: KLOTHO NBP1-76511 (2 ug/mL), KLOTHO, competitor antibody (4 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Klotho-Antibody---BSA-Free-Western-Blot-NBP1-76511-img0009.jpg)
![Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Blockade of the HIF‐1 alpha /p53/miRNA‐34a/Klotho axis decreases the leakage & area of mouse laser‐induced CNV. The mice were divided into the following groups: normal, CNV 7 d, CNV 7 d + 0.1% DMSO, CNV 7 d + digoxin (oral; 2 mg/kg for 7 d), CNV 7 d + AAV‐p53 mutant (intravitreal injection; approximately 3 μL, 3 × 1010 viral particles/mL), CNV 7 d + miRNA‐34a inhibitor (intravitreal injection; 1 μg) & CNV 7 d + AAV‐Klotho full‐length plasmid (intravitreal injection, 2 μL, 5 × 1010 viral particles/mL). A, Western blot was performed to measure HIF‐1 alpha , p‐p53 (S15), p53 (S20), p‐p53 (S46), p53 & Klotho protein levels. B, The relative protein levels of HIF‐1 alpha /GAPDH (B), p‐p53 (S15)/p53 (C), p‐p53 (S20)/p53 (D), p‐p53 (S46)/p53 (E), p53/GAPDH (F) & Klotho/GAPDH (G) were analysed. ***P < .001, CNV 7‐d group vs normal group. ##P < .01, compared with the CNV 7‐d group. NS, CNV 7 d + KL OE group vs CNV 7‐d group. H, RT‐PCR was performed to measure the expression of miRNA‐34a. **P < .01, CNV 7‐d group vs normal group. ##P < .01, compared with the CNV 7‐d group. NS, CNV 7 d + KL OE group vs CNV 7‐d group. I, FFA was performed to measure the leakage of CNV. J, The leakage of CNV was analysed. K, ICGA was performed to measure the area of CNV. L, The area of CNV was analysed. **P < .01, ***P < .001, compared with the CNV 7‐d group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33438362), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-76511_rabbit-polyclonal-klotho-antibody-310202416171454.jpg)
![Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - microrna‐34a inhibits the expression of Klotho. ARPE‐19 cells were divided into the following groups: normal, hypoxia, hypoxia + AAV vector, hypoxia + digoxin, hypoxia + digoxin + nutlin‐3a, hypoxia + AAV‐p53 mutant infection & hypoxia + miRNA‐34a inhibitor. A, Western blot assay of Klotho in ARPE‐19 cells was performed. B, The relative protein level of Klotho compared with the GAPDH level was analysed. ***P < .001, hypoxia group vs normal group. ###P < .001, ##P < .01, compared with the hypoxia group. %%P < .01, hypoxia + digoxin + nutlin‐3a group vs hypoxia + digoxin group. C, Schematic of the Klotho 3' UTR with the WT or mutated putative‐binding site of miRNA‐34a inserted into a luciferase (Luc) reporter. D, qRT‐PCR analysis of Klotho mRNA expression in HEK293T cells transfected with miRNA‐34a mimic for 24 h. **P < .01, miRNA‐34a mimic group vs control group. E, Western blot of Klotho in HEK293T cells transfected with a miRNA‐34a mimic for 24 h was performed. F, Immunoblotted Klotho was quantified & normalized to GAPDH. ***P < .001, miRNA‐34a mimic group vs control group. G Relative luciferase activity was detected in HEK293T cells co‐transfected with plasmids containing firefly luciferase & wild‐type (WT) or mutant (Mut) Klotho 3′‐UTR & miRNA‐34a mimic for 24 h. The luciferase activity values were normalized to Renilla reniformis luciferase (TK‐RL) activity. **P < .01, Luc‐Klotho 3′ UTR WT group vs vector group. Statistically non‐significant (NS), Luc‐Klotho 3′ UTR mutant group vs vector group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33438362), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-76511_rabbit-polyclonal-klotho-antibody-310202416154634.jpg)
![Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - High phytate-induced renal phosphate wasting is independent of FGF23.(A) The renal expression of downregulated genes associated with Ca2+ & phosphate homeostasis in rats fed control or phytate-supplemented diets (n = 8 per group). (B) Results of immunoblot analyses & protein levels of renal aKlotho, NHERF1, & NaPi-2a after 2 weeks for rats fed control, HP- LCa2+ , & HP-HCa2+ diets. (C) The levels of renal proteins were quantified using ImageJ software (n = 6 per group). (D, E) Time course analysis of serum levels of intact FGF23 (D) & C-terminal FGF23 (E) in rats fed control, HP-LCa2+ , & HP-HCa2+ diets. (F) Representative immunohistochemical staining of EDTA-decalcified femur sections for FGF23 from rats fed control, HP-LCa2+ , & HP-HCa2+ diets. All data are presented as the mean ± SD of each group (n = 3–8 per group). *, p<0.05; **, p<0.01; ***, p<0.001, compared with controls. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32271147), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-76511_rabbit-polyclonal-klotho-antibody-310202416171414.jpg)
![Age-related declines in alpha -Klotho are associated with cartilage degeneration in male mice. A Aging induced a progressive decline of alpha -Klotho in the murine medial tibia (n = 5/sex/age). White arrows indicate alpha -Klotho-positive chondrocytes. White dashed lines indicate cartilage surface. AC articular cartilage. Scale bar: 10 μm. alpha -Klotho expression per cell was quantified by immunofluorescence (50–100 cells per mouse). B Cartilage in older adults (≥65 years old; 71.9 ± 2.91 years; n = 7 [1 female]) displayed reduced alpha -Klotho expression compared to young adults (<40 years old; 27.6 ± 6.85 years; n = 7 [2 females]). Scale bar: 20 μm. alpha -Klotho expression per cell was quantified by immunofluorescence (30–70 cells per cartilage sample). C Loss-of function in Klotho (Klotho+/−) triggered murine cartilage degeneration in a sex-dependent manner (young, n = 7 for wild-type [3 females], n = 10 for Klotho+/− [5 females]; middle-aged, n = 10 for wild-type [5 females], n = 8 for Klotho+/− [5 females]). Black arrows indicate cartilage surface disruption. AC articular cartilage. Scale bar: 50 μm. Statistical analyses were performed using linear regression (A), two-way ANOVA (C), and a two-tailed Student t test (B). Age-sex interaction was not significant for alpha -Klotho expression in mice (A, p = 0.468). Data are presented as means ± 95% confidence intervals. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36627269), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab1819_mouse-klotho-mab-clone-236214-immunocytochemistry-immunofluorescence-12122025916596.jpg)
![Age-related declines in alpha -Klotho are associated with cartilage degeneration in male mice. A Aging induced a progressive decline of alpha -Klotho in the murine medial tibia (n = 5/sex/age). White arrows indicate alpha -Klotho-positive chondrocytes. White dashed lines indicate cartilage surface. AC articular cartilage. Scale bar: 10 μm. alpha -Klotho expression per cell was quantified by immunofluorescence (50–100 cells per mouse). B Cartilage in older adults (≥65 years old; 71.9 ± 2.91 years; n = 7 [1 female]) displayed reduced alpha -Klotho expression compared to young adults (<40 years old; 27.6 ± 6.85 years; n = 7 [2 females]). Scale bar: 20 μm. alpha -Klotho expression per cell was quantified by immunofluorescence (30–70 cells per cartilage sample). C Loss-of function in Klotho (Klotho+/−) triggered murine cartilage degeneration in a sex-dependent manner (young, n = 7 for wild-type [3 females], n = 10 for Klotho+/− [5 females]; middle-aged, n = 10 for wild-type [5 females], n = 8 for Klotho+/− [5 females]). Black arrows indicate cartilage surface disruption. AC articular cartilage. Scale bar: 50 μm. Statistical analyses were performed using linear regression (A), two-way ANOVA (C), and a two-tailed Student t test (B). Age-sex interaction was not significant for alpha -Klotho expression in mice (A, p = 0.468). Data are presented as means ± 95% confidence intervals. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36627269), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab1819_mouse-klotho-mab-clone-236214-immunocytochemistry-immunofluorescence-1212202585287.jpg)
![Age-related declines in alpha -Klotho are associated with cartilage degeneration in male mice. A Aging induced a progressive decline of alpha -Klotho in the murine medial tibia (n = 5/sex/age). White arrows indicate alpha -Klotho-positive chondrocytes. White dashed lines indicate cartilage surface. AC articular cartilage. Scale bar: 10 μm. alpha -Klotho expression per cell was quantified by immunofluorescence (50–100 cells per mouse). B Cartilage in older adults (≥65 years old; 71.9 ± 2.91 years; n = 7 [1 female]) displayed reduced alpha -Klotho expression compared to young adults (<40 years old; 27.6 ± 6.85 years; n = 7 [2 females]). Scale bar: 20 μm. alpha -Klotho expression per cell was quantified by immunofluorescence (30–70 cells per cartilage sample). C Loss-of function in Klotho (Klotho+/−) triggered murine cartilage degeneration in a sex-dependent manner (young, n = 7 for wild-type [3 females], n = 10 for Klotho+/− [5 females]; middle-aged, n = 10 for wild-type [5 females], n = 8 for Klotho+/− [5 females]). Black arrows indicate cartilage surface disruption. AC articular cartilage. Scale bar: 50 μm. Statistical analyses were performed using linear regression (A), two-way ANOVA (C), and a two-tailed Student t test (B). Age-sex interaction was not significant for alpha -Klotho expression in mice (A, p = 0.468). Data are presented as means ± 95% confidence intervals. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36627269), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab1819_mouse-klotho-mab-clone-236214-immunocytochemistry-immunofluorescence-12122025981219.jpg)
![Age-related declines in alpha -Klotho are associated with cartilage degeneration in male mice. A Aging induced a progressive decline of alpha -Klotho in the murine medial tibia (n = 5/sex/age). White arrows indicate alpha -Klotho-positive chondrocytes. White dashed lines indicate cartilage surface. AC articular cartilage. Scale bar: 10 μm. alpha -Klotho expression per cell was quantified by immunofluorescence (50–100 cells per mouse). B Cartilage in older adults (≥65 years old; 71.9 ± 2.91 years; n = 7 [1 female]) displayed reduced alpha -Klotho expression compared to young adults (<40 years old; 27.6 ± 6.85 years; n = 7 [2 females]). Scale bar: 20 μm. alpha -Klotho expression per cell was quantified by immunofluorescence (30–70 cells per cartilage sample). C Loss-of function in Klotho (Klotho+/−) triggered murine cartilage degeneration in a sex-dependent manner (young, n = 7 for wild-type [3 females], n = 10 for Klotho+/− [5 females]; middle-aged, n = 10 for wild-type [5 females], n = 8 for Klotho+/− [5 females]). Black arrows indicate cartilage surface disruption. AC articular cartilage. Scale bar: 50 μm. Statistical analyses were performed using linear regression (A), two-way ANOVA (C), and a two-tailed Student t test (B). Age-sex interaction was not significant for alpha -Klotho expression in mice (A, p = 0.468). Data are presented as means ± 95% confidence intervals. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36627269), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab7155_mouse-ikk-beta-mab-clone-725818-western-blot-121220258481615.jpg)
![N/A Klotho [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Klotho_DY533405_ELISA_2477.jpg "N/A Klotho [Biotin]")
![Immunohistochemistry-Paraffin: Klotho Antibody [NB100-2432] - Staining of Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/Klotho-Antibody-Immunohistochemistry-Paraffin-NB100-2432-img0007.jpg "Immunohistochemistry-Paraffin: Klotho Antibody [NB100-2432] - Staining of Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.")
![Immunohistochemistry-Paraffin: Klotho Antibody [NB100-2432] - Staining of Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/Klotho-Antibody-Immunohistochemistry-Paraffin-NB100-2432-img0005.jpg)
![ELISA: Mouse Klotho ELISA Kit (Colorimetric) [NBP3-18030] - Samples were spiked with high concentrations of Mouse Klotho and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-18030_mouse-klotho-elisa-kit-colorimetric-13220241612139.jpg "ELISA: Mouse Klotho ELISA Kit (Colorimetric) [NBP3-18030] - Samples were spiked with high concentrations of Mouse Klotho and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Klotho ELISA Kit (Colorimetric) [NBP3-18030] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-18030_mouse-klotho-elisa-kit-colorimetric-432024924058.jpg)
![Western Blot: Klotho Antibody [NBP1-55097] - Titration: 0.2-1 ug/ml, Positive Control: Human Thymus.](http://images.novusbio.com/fullsize/Klotho-Antibody-Western-Blot-NBP1-55097-img0003.jpg "Western Blot: Klotho Antibody [NBP1-55097] - Titration: 0.2-1 ug/ml, Positive Control: Human Thymus.")
![Western Blot: Klotho Overexpression Lysate (Adult Normal) [NBL1-12307] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Klotho.](http://images.novusbio.com/fullsize/Klotho-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12307-img0002.jpg "Western Blot: Klotho Overexpression Lysate (Adult Normal) [NBL1-12307] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Klotho.")
![Western Blot: Klotho Antibody (D1) [NBP3-42104] - Recombinant Rat Klotho](http://images.novusbio.com/fullsize/nbp3-42104_mouse-klotho-mab-d1-1412025151380.jpg "Western Blot: Klotho Antibody (D1) [NBP3-42104] - Recombinant Rat Klotho")
