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HAF109). A specific band was detected for Klotho at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
MAB1819) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Klotho Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1819) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox" target="_blank">
DY990). Goat Anti-Mouse Klotho Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1819) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox" target="_blank">
2604-FG) is immobilized at 5 µg/mL, 100 µL/well, Recombinant Human Klotho Mucin Stalk Chimera His-tag (Catalog # 10308-KL) binds with an ED50 of 0.2-1.8 µg/mL." class="big_lightbox" target="_blank">
The Klotho gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (