Klotho Antibody (775340)

Images

 
Recombinant Human Klotho (Catalog # 5334-KL) induces proliferation in the BaF3 mouse pro‑B cell line transfected with human FGF RIIIc in a dose-dependent manner (orange line). Proliferation elicited by Recombinant ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications B/N
Clone
775340
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Klotho Antibody (775340) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Klotho
Glu34-Ser981
Accession # Q9UEF7
Specificity
Detects human Klotho in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) Klotho (aa 35-982) is observed and no cross-reactivity with rmKlotho (aa 23-550), recombinant human (rh) Klotho beta (aa 53-997), or rhCytosolic beta‑Glucosidase/GBA3 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
KL
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Neutralization 0.15-0.6 ug/mL
Publications
Read Publications using
MAB5334 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Klotho Antibody (775340)

  • EC 3.2.1
  • EC 3.2.1.31
  • KL
  • Klotho

Background

Klotho, also called Klotho-alpha , is the founding member of the Klotho family within the glycosidase-1 superfamily (1, 2). Klotho is expressed in areas concerned with calcium regulation, predominantly in the kidney distal convoluted tubules, but also in the brain choroid plexus (which produces cerebrospinal fluid) and the parathyroid (1). The 1012 amino acid (aa) type I transmembrane protein contains a 32 aa signal sequence, a 948 aa extracellular domain (ECD) containing two extracellular glycosidase-like domains, a 21 aa transmembrane domain and an 11 aa intracellular domain. Within the ECD, human Klotho shares 87%, 90%, 90% and 86% aa identity with mouse, rat, bovine and equine Klotho, respectively. Although a truncated 549 aa isoform predicts a soluble 70 kDa form, the form found in plasma and cerebrospinal fluid is a 130 kDa form produced by proteolytic cleavage of the glycosylated 135 kDa full-length Klotho (3, 4). A prominent intracellular 120 kDa form of Klotho is localized to endoplasmic reticulum and Golgi membranes (4). Klotho is named for the Greek goddess who spins the thread of life. The phenotype of Klotho‑deficient mice resembles premature aging, including arteriosclerosis, osteoporosis, skin atrophy, infertility, emphysema and premature death (2). Conversely, excess Klotho extends lifespan (5). Klotho acts as a cofactor for interaction of FGF23 with FGF R1 (6). This interaction negatively regulates 1 alpha -hydroxylase, the rate‑limiting enzyme in the synthesis of 1,25(OH)2D3 (vitamin D)(7). Klotho‑deficient mice show severe hyperphosphatemia and ectopic calcification of soft tissues due to excess vitamin D (2, 7). Both Klotho and Klotho-beta are cofactors for FGF19 binding (8). Klotho also shows glucuronidase activity which activates the renal ion channel TRPV5 to reabsorb urinary calcium (9). Klotho has been reported to downregulate insulin or IGF-1 signaling in adipocytes, to bind and antagonize Wnt molecules, and to facilitate release of parathyroid hormone (10-12).
  1. Nabeshima, Y. (2006) Sci. Aging Knowl. Environ. 8:pe11.
  2. Kuro-o, M. et al. (1997) Nature 390:45.
  3. Shiraki-Iida, T. et al. (1998) FEBS Lett. 424:6.
  4. Imura, A. et al. (2004) FEBS Lett. 565:143.
  5. Kurosu, H. et al. (2005) Science 309:1829.
  6.  Kurosu, H. et al. (2006) J. Biol. Chem. 281:6120.
  7. Tsujikawa, H. et al. (2003) Mol. Endocrinol. 17:2393.
  8. Wu, X. et al. (2007) J. Biol. Chem. 282:29069.
  9. Chang, Q. et al. (2005) Science 310:490.
  10. Yamamoto, M. et al. (2005) J. Biol. Chem. 280:38029.
  11. Liu, H. et al. (2007) Science 317:803.
  12. Imura, A. et al. (2007) Science 316:1615.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

7466-SC/CF
Species: Hu
Applications: BA
2629-FG/CF
Species: Mu
Applications: BA
203-IL/CF
Species: Hu
Applications: BA
MAB7665
Species: Hu
Applications: IHC, WB
D6050
Species: Hu
Applications: ELISA
308-FK/CF
Species: Hu
Applications: BA
288-TPN/CF
Species: Hu
Applications: BA
NB600-1071
Species: Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
AF1356
Species: Hu, Mu
Applications: CyTOF-ready, Flow, IHC, Simple Western, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
H00138428-P01
Species: Hu
Applications: ELISA, AP, PA, WB
MEP00B
Species: Mu
Applications: ELISA
7954-GM/CF
Species: Hu
Applications: BA
7954-GM/CF
Species: Hu
Applications: BA
NBP2-80739
Species: Hu
Applications: CyTOF-ready, Flow, ICC, IHC, IHC-P, WB
207-IL/CF
Species: Hu
Applications: BA
NBP1-80670
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-92637
Species: Hu, Rt
Applications: ICC/IF, WB

Publications for Klotho Antibody (MAB5334)(2)

Reviews for Klotho Antibody (MAB5334) (0)

There are no reviews for Klotho Antibody (MAB5334). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Klotho Antibody (MAB5334). (Showing 1 - 1 of 1 FAQs).

  1. I study physiology (PhD) and I need a klotho kit.
    • A list of our klotho products can be found here. I am not entirely sure what you are looking for as I don't have your complete message for some reason. Can you please clarify what you are looking for?

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional Klotho Products

Bioinformatics Tool for Klotho Antibody (MAB5334)

Discover related pathways, diseases and genes to Klotho Antibody (MAB5334). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Klotho Antibody (MAB5334)

Discover more about diseases related to Klotho Antibody (MAB5334).
 

Pathways for Klotho Antibody (MAB5334)

View related products by pathway.

PTMs for Klotho Antibody (MAB5334)

Learn more about PTMs related to Klotho Antibody (MAB5334).

Blogs on Klotho

There are no specific blogs for Klotho, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Klotho Antibody (775340) and receive a gift card or discount.

Bioinformatics

Gene Symbol KL
Uniprot