Klotho Antibody (236214) [Unconjugated]

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Recombinant Mouse Klotho protein was serially diluted 2-fold and captured by Rat Anti-Mouse Klotho Monoclonal Antibody (Catalog # MAB1819) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA, IHC
Clone
236214
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Klotho Antibody (236214) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Klotho
Specificity
Detects mouse Klotho (aa 23-550 and aa 35-982) in direct ELISAs and Western blots. Does not cross-react with recombinant human Klotho or recombinant mouse Klotho B.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
KL
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry 8-25 ug/mL
Publications
Read Publication using
MAB1819 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Klotho Antibody (236214) [Unconjugated]

  • EC 3.2.1
  • EC 3.2.1.31
  • KL
  • Klotho

Background

Klotho, also called Klotho-alpha , is the founding member of the Klotho family within the glycosidase-1 superfamily (1, 2). Klotho is expressed in areas concerned with calcium regulation, predominantly in the kidney distal convoluted tubules, but also in the brain choroid plexus (which produces cerebrospinal fluid) and the parathyroid (1). The 1014 amino acid (aa) type I transmembrane protein contains a 34 aa signal sequence, a 948 aa extracellular domain (ECD) containing two extracellular glycosidase-like domains, a 21 aa transmembrane domain and an 11 aa intracellular domain. Within the ECD, mouse Klotho shares 95%, 87%, and 87% aa identity with rat, human, and equine Klotho, respectively. Although a truncated 554 aa isoform predicts a soluble 70 kDa form, the soluble form found in plasma and cerebrospinal fluid is a 130 kDa form produced by proteolytic cleavage of the glycosylated 135 kDa full-length Klotho (3, 4). A prominent intracellular 120 kDa form of Klotho is localized to endoplasmic reticulum and Golgi membranes (4). Klotho is named for the Greek goddess who spins the thread of life. The phenotype of Klotho-deficient mice resembles premature aging, including arteriosclerosis, osteoporosis, skin atrophy, infertility, emphysema, and premature death (2). Conversely, excess Klotho extends lifespan (5). Klotho acts as a cofactor for interaction of FGF-23 with FGF R1 (6). This interaction negatively regulates 1 alpha -hydroxylase, the rate-limiting enzyme in the synthesis of 1,25(OH)2D3 (vitamin D) (7). Klotho-deficient mice show severe hyperphosphatemia and ectopic calcification of soft tissues due to excess vitamin D (2, 7). Both Klotho and Klotho-beta are co-factors for FGF-19 binding (8). Klotho also shows glucuronidase activity which activates the renal ion channel TRPV5 to reabsorb urinary calcium (9). Klotho has been reported to downregulate insulin or IGF-I signaling in adipocytes, to bind and antagonize Wnt molecules, and to facilitate release of parathyroid hormone (10-12).

  1. Nabeshima, Y. (2006) Sci. Aging Knowl. Environ. 8:pe11. 
  2. Kuro-o, M. et al. (1997) Nature 390:45. 
  3. Shiraki-Iida, T. et al. (1998) FEBS Lett. 424:6. 
  4. Imura, A. et al. (2004) FEBS Lett. 565:143. 
  5. Kurosu, H. et al. (2005) Science 309:1829. 
  6. Kurosu, H. et al. (2006) J. Biol. Chem. 281:6120. 
  7. Tsujikawa, H. et al. (2003) Mol. Endocrinol. 17:2393.
  8. Wu, X. et al. (2007) J. Biol. Chem. 282:29069.
  9. Chang, Q. et al. (2005) Science 310:490.
  10. Yamamoto, M. et al. (2005) J. Biol. Chem. 280:38029.
  11. Liu, H. et al. (2007) Science 317:803.
  12. Imura, A. et al. (2007) Science 316:1615.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Klotho Antibody (MAB1819)(1)

We have publications tested in 1 confirmed species: C. elegans.

We have publications tested in 1 application: Immunoprecipitation.


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Bioinformatics

Gene Symbol KL