KIR2DL1/KIR2DS5 Antibody (143211) [Unconjugated]

Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human KIR2DL1/KIR2DS5 Monoclonal Antibody (Catalog # MAB1844) or (B) Mouse IgG1 Isotype Control Antibody (Catalog # MAB002) followed by Anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-reported
• Clone: 143211
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

KIR2DL1/KIR2DS5 Antibody (143211) [Unconjugated] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with human KIR2DL1/KIR2DS5
  Accession # P43626
• Specificity: Detects human KIR2DL1/KIR2DS5 on transfected cells. It does not stain cells transfected with KIR2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 2DS4, 3DL1, 3DL2, or 3DS1.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KIR2DL1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-reported
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KIR2DL1/KIR2DS5 Antibody (143211) [Unconjugated]

• CD158a
• KIR2DL1/KIR2DS5

Background

KIR2DL1 is one of several immunoglobulin-like receptors expressed on NK cells that bind MHC class I molecules and transmit inhibitory signals. KIR2DL1 is specific for HLA-C alleles with Asn77 and Lys80.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KIR2DL1/KIR2DS5 Antibody (MAB1844)(14)

Publications using MAB1844

• L Dubreuil, B Maniangou, P Chevallier, A Quéméner, N Legrand, MC Béné, C Willem, G David, M Alizadeh, DR Makanga, A Cesbron, K Gendzekhad, K Gagne, C Retière Centromeric KIR AA Individuals Harbor Particular KIR Alleles Conferring Beneficial NK Cell Features with Implications in Haplo-Identical Hematopoietic Stem Cell Transplantation Cancers, 2020;12(12):. 2020 [PMID: 33271841] (Flow Cytometry, Human)
• L Gimeno, EM Serrano-Ló, JA Campillo, MA Cánovas-Za, OS Acuña, F García-Cóz, MV Martínez-S, MD Martínez-H, MF Soto-Ramír, P López-Cubi, J Martínez-E, J Martínez-G, S Corbalan-G, MR Álvarez-Ló, A Minguela KIR+ CD8+ T Lymphocytes in Cancer Immunosurveillance and Patient Survival: Gene Expression Profiling Cancers (Basel), 2020;12(10):. 2020 [PMID: 33076479] (Flow Cytometry, Human)
• A Koenig, CC Chen, A Marçais, T Barba, V Mathias, A Sicard, M Rabeyrin, M Racapé, JP Duong-Van-, P Bruneval, A Loupy, S Dussurgey, S Ducreux, V Meas-Yedid, JC Olivo-Mari, H Paidassi, R Guillemain, JL Taupin, J Callemeyn, E Morelon, A Nicoletti, B Charreau, V Dubois, M Naesens, T Walzer, T Defrance, O Thaunat Missing self triggers NK cell-mediated chronic vascular rejection of solid organ transplants Nat Commun, 2019;10(1):5350. 2019 [PMID: 31767837] (Flow Cytometry, Human)
• AB Kristensen, SJ Kent, MS Parsons Contribution of NK cell education to both direct and anti-HIV-1 antibody-dependent NK cell functions J. Virol., 2018;0(0):. 2018 [PMID: 29514913] (Flow Cytometry, Human)
• S Nguyen, A Achour, L Souchet, S Vigouroux, P Chevallier, S Furst, A Sirvent, JO Bay, G Socié, P Ceballos, A Huynh, J Cornillon, S Francois, F Legrand, I Yakoub-Agh, G Michel, N Maillard, G Margueritt, S Maury, M Uzunov, CE Bulabois, M Michallet, L Clement, C Dauriac, K Bilger, J Lejeune, V Béziat, V Rocha, B Rio, S Chevret, V Vieillard Clinical impact of NK-cell reconstitution after reduced intensity conditioned unrelated cord blood transplantation in patients with acute myeloid leukemia: analysis of a prospective phase II multicenter trial on behalf of the Soci�t� Fran�aise de Greffe de Moelle Osseuse et Th�rapie Cellulaire and Eurocord Bone Marrow Transplant., 2017;0(0):. 2017 [PMID: 28650455] (Flow Cytometry, Human)
• MS Malnati, E Ugolotti, MC Monti, D Battista, I Vanni, D Bordo, F Sironi, P Larghero, ED Marco, P Biswas, G Poli, E Vicenzi, A Riva, M Tarkowski, G Tambussi, S Nozza, G Tripodi, F Marras, A Maria, A Pistorio, R Biassoni Activating Killer Immunoglobulin Receptors and HLA-C: a successful combination providing HIV-1 control Sci Rep, 2017;7(0):42470. 2017 [PMID: 28211903] (Flow Cytometry, Human)
• Raphaëlle Riou Severe symptomatic primary HCMV infection despite effective innate and adaptive immune responses J. Virol, 2016;0(0):. 2016 [PMID: 28031361] (Flow Cytometry, Human)
• Ashley Moffett Activating KIR2DS4 Is Expressed by Uterine NK Cells and Contributes to Successful Pregnancy J. Immunol., 2016;0(0):. 2016 [PMID: 27815424] (Flow Cytometry, Human)
• Horowitz A, Guethlein L, Nemat-Gorgani N, Norman P, Cooley S, Miller J, Parham P Regulation of Adaptive NK Cells and CD8 T Cells by HLA-C Correlates with Allogeneic Hematopoietic Cell Transplantation and with Cytomegalovirus Reactivation. J Immunol, 2015;195(9):4524-36. 2015 [PMID: 26416275] (CyTof, Human)
• Enqvist M, Ask E, Forslund E, Carlsten M, Abrahamsen G, Beziat V, Andersson S, Schaffer M, Spurkland A, Bryceson Y, Onfelt B, Malmberg K Coordinated expression of DNAM-1 and LFA-1 in educated NK cells. J Immunol, 2015;194(9):4518-27. 2015 [PMID: 25825444] (Flow Cytometry, Human)
• Huijskens M, Walczak M, Sarkar S, Atrafi F, Senden-Gijsbers B, Tilanus M, Bos G, Wieten L, Germeraad W Ascorbic acid promotes proliferation of natural killer cell populations in culture systems applicable for natural killer cell therapy. Cytotherapy, 0;17(5):613-20. 0 [PMID: 25747742] (Flow Cytometry, Human)
• Charoudeh H, Terszowski G, Czaja K, Gonzalez A, Schmitter K, Stern M Modulation of the natural killer cell KIR repertoire by cytomegalovirus infection. Eur J Immunol, 2013;43(2):480-7. 2013 [PMID: 23161492] (Flow Cytometry, Human)
• Denman CJ, Senyukov VV, Somanchi SS Membrane-bound IL-21 promotes sustained ex vivo proliferation of human natural killer cells. PLoS ONE, 2012;7(1):e30264. 2012 [PMID: 22279576] (Flow Cytometry, Human)
• Bari R, Bell T, Leung WH, Vong QP, Chan WK, Das Gupta N, Holladay M, Rooney B, Leung W Significant functional heterogeneity among KIR2DL1 alleles and a pivotal role of arginine 245. Blood, 2009;114(25):5182-90. 2009 [PMID: 19828694] (ICC, Human)

Bioinformatics

• Gene Symbol: KIR2DL1
• Uniprot:
  • P43626()