KIR2DL1/KIR2DS5 Antibody (143211) [Alexa Fluor® 700]

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human KIR2DL1/KIR2DS5 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB1844N) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). Quadrant markers were set based on control antibody staining (Catalog # IC002N). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 143211
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

KIR2DL1/KIR2DS5 Antibody (143211) [Alexa Fluor® 700] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with human KIR2DL1/KIR2DS5
  Accession # P43626
• Specificity: Detects human KIR2DL1/KIR2DS5 on transfected cells. It does not stain cells transfected with KIR2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 2DS4, 3DL1, 3DL2, or 3DS1.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KIR2DL1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KIR2DL1/KIR2DS5 Antibody (143211) [Alexa Fluor® 700]

• CD158a
• KIR2DL1/KIR2DS5

Background

KIR2DL1 is one of several immunoglobulin-like receptors expressed on NK cells that bind MHC class I molecules and transmit inhibitory signals. KIR2DL1 is specific for HLA-C alleles with Asn77 and Lys80.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KIR2DL1/KIR2DS5 Antibody (FAB1844N)(4)

Publications using FAB1844N

• VA Vieira, E Adland, DFG Malone, MP Martin, A Groll, MA Ansari, MC Garcia-Gue, MC Puertas, M Muenchhoff, CF Guash, C Brander, J Martinez-P, A Bamford, G Tudor-Will, T Ndung'u, BD Walker, V Ramsuran, J Frater, P Jooste, D Peppa, M Carrington, PJR Goulder An HLA-I signature favouring KIR-educated Natural Killer cells mediates immune control of HIV in children and contrasts with the HLA-B-restricted CD8+ T-cell-mediated immune control in adults PloS Pathogens, 2021-11-18;17(11):e1010090. 2021-11-18 [PMID: 34793581] (Flow Cytometry, Human)
• I Lisovsky, S Kant, A Tremblay-M, G Isitman, Z Kiani, FP Dupuy, L Gilbert, J Bruneau, NH Shoukry, B Lebouché, NF Bernard Differential contribution of education through KIR2DL1, KIR2DL3, and KIR3DL1 to antibody-dependent (AD) NK cell activation and ADCC J. Leukoc. Biol., 2019-01-30;0(0):. 2019-01-30 [PMID: 30698860] (Flow Cytometry, Human)
• D Feyaerts, T Kuret, B van Cranen, S van der Ze, OWH van der He, A van der Me, I Joosten, RG van der Mo Endometrial natural killer (NK) cells reveal a tissue-specific receptor repertoire Hum. Reprod., 2018-03-01;0(0):. 2018-03-01 [PMID: 29447367] (Flow Cytometry, Human)
• Charoudeh H, Terszowski G, Czaja K, Gonzalez A, Schmitter K, Stern M Modulation of the natural killer cell KIR repertoire by cytomegalovirus infection. Eur J Immunol, 2012-12-12;43(2):480-7. 2012-12-12 [PMID: 23161492] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: KIR2DL1
• Uniprot:
  • P43626()