KIR2DL1/KIR2DS5 Antibody (143211) [Fluorescein]

Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human KIR2DL1/KIR2DS5 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1844F) or (B) Mouse IgG1 Isotype Control Antibody (Catalog # IC002F) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 143211
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

KIR2DL1/KIR2DS5 Antibody (143211) [Fluorescein] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with human KIR2DL1/KIR2DS5
  Accession # P43626
• Specificity: Detects human KIR2DL1/KIR2DS5 on transfected cells. It does not stain cells transfected with KIR2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 2DS4, 3DL1, 3DL2, or 3DS1.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KIR2DL1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KIR2DL1/KIR2DS5 Antibody (143211) [Fluorescein]

• CD158a
• KIR2DL1/KIR2DS5

Background

KIR2DL1 is one of several immunoglobulin-like receptors expressed on NK cells that bind MHC class I molecules and transmit inhibitory signals. KIR2DL1 is specific for HLA-C alleles with Asn77 and Lys80.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KIR2DL1/KIR2DS5 Antibody (FAB1844F)(13)

Publications using FAB1844F

• Johannes Höfle, Timo Trenkner, Nadja Kleist, Vera Schwane, Sarah Vollmers, Bryan Barcelona et al. Engagement of TRAIL triggers degranulation and IFN gamma production in human natural killer cells EMBO reports 8/3/2022 [PMID: 35758160]
• V Mikhailova, P Grebenkina, E Khokhlova, A Davydova, Z Salloum, E Tyshchuk, V Zagainova, K Markova, I Kogan, S Selkov, D Sokolov Pro- and Anti-Inflammatory Cytokines in the Context of NK Cell-Trophoblast Interactions International Journal of Molecular Sciences, 2022-02-21;23(4):. 2/21/2022 [PMID: 35216502]
• Grzegorz Terszowski Human Cytomegalovirus Infection Enhances NK Cell Activity In Vitro Transplant Direct, 2016-06-17;2(7):e89. 2016-06-17 [PMID: 27830183] (Flow Cytometry, Human)
• P Rettman, C Willem, G David, R Riou, N Legrand, J Esbelin, A Cesbron, D Senitzer, K Gagne, C Retière New insights on the natural killer cell repertoire from a thorough analysis of cord blood cells J Leukoc Biol, 2016-04-11;0(0):. 2016-04-11 [PMID: 27067451] (Flow Cytometry, Human)
• Sharkey A, Xiong S, Kennedy P, Gardner L, Farrell L, Chazara O, Ivarsson M, Hiby S, Colucci F, Moffett A Tissue-Specific Education of Decidual NK Cells. J Immunol, 2015-08-28;195(7):3026-32. 2015-08-28 [PMID: 26320253] (Flow Cytometry, Human)
• Marquardt N, Ivarsson M, Blom K, Gonzalez V, Braun M, Falconer K, Gustafsson R, Fogdell-Hahn A, Sandberg J, Michaelsson J The Human NK Cell Response to Yellow Fever Virus 17D Is Primarily Governed by NK Cell Differentiation Independently of NK Cell Education. J Immunol, 2015-08-17;195(7):3262-72. 2015-08-17 [PMID: 26283480] (Flow Cytometry, Human)
• Pesce S, Carlomagno S, Moretta A, Sivori S, Marcenaro E Uptake of CCR7 by KIR2DS4(+) NK cells is induced upon recognition of certain HLA-C alleles. J Immunol Res, 2015-04-16;2015(0):754373. 2015-04-16 [PMID: 25961063] (Flow Cytometry, Human)
• Dunphy S, Guinan K, Chorcora C, Jayaraman J, Traherne J, Trowsdale J, Pende D, Middleton D, Gardiner C 2DL1, 2DL2 and 2DL3 all contribute to KIR phenotype variability on human NK cells. Genes Immun, 2015-05-07;16(5):301-10. 2015-05-07 [PMID: 25950617] (Flow Cytometry, Human)
• Terszowski G, Klein C, Stern M KIR/HLA interactions negatively affect rituximab- but not GA101 (obinutuzumab)-induced antibody-dependent cellular cytotoxicity. J Immunol, 2014-05-02;192(12):5618-24. 2014-05-02 [PMID: 24795454] (Flow Cytometry, Human)
• Achour , Abla, Baychelier , Florence, Besson , Caroline, Arnoux , Armelle, Marty , Michel, Hannoun , Laurent, Samuel , Didier, Debre , Patrice, Vieillard , Vincent Expansion of CMV-mediated NKG2C+ NK cells associates with the development of specific de novo malignancies in liver-transplanted patients. J Immunol, 2013-12-04;192(1):503-11. 2013-12-04 [PMID: 24307732] (Flow Cytometry, Human)
• Charoudeh H, Terszowski G, Czaja K, Gonzalez A, Schmitter K, Stern M Modulation of the natural killer cell KIR repertoire by cytomegalovirus infection. Eur J Immunol, 2012-12-12;43(2):480-7. 2012-12-12 [PMID: 23161492] (Flow Cytometry, Human)
• Eller MA, Koehler RN, Kijak GH Human Immunodeficiency Virus Type 1 Infection Is Associated with Increased NK Cell Polyfunctionality and Higher Levels of KIR3DL1+ NK Cells in Ugandans Carrying the HLA-B Bw4 Motif. J. Virol., 2011-03-16;85(10):4802-11. 2011-03-16 [PMID: 21411516] (Flow Cytometry, Human)
• Bjorkstrom NK, Lindgren T, Stoltz M, Fauriat C, Braun M, Evander M, Michaelsson J, Malmberg KJ, Klingstrom J, Ahlm C, Ljunggren HG Rapid expansion and long-term persistence of elevated NK cell numbers in humans infected with hantavirus. J. Exp. Med., 2010-12-20;208(1):13-21. 2010-12-20 [PMID: 21173105] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: KIR2DL1
• Uniprot:
  • P43626()