KIR2DL1/KIR2DS5 Antibody (143211) [Allophycocyanin]

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human KIR2DL1/KIR2DS5 APC-conjugated Monoclonal Antibody (Catalog # FAB1844A) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 143211
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

KIR2DL1/KIR2DS5 Antibody (143211) [Allophycocyanin] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with human KIR2DL1/KIR2DS5
  Accession # P43626
• Specificity: Detects human KIR2DL1/KIR2DS5 on transfected cells. It does not stain cells transfected with KIR2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 2DS4, 3DL1, 3DL2, or 3DS1.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KIR2DL1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KIR2DL1/KIR2DS5 Antibody (143211) [Allophycocyanin]

• CD158a
• KIR2DL1/KIR2DS5

Background

KIR2DL1 is one of several immunoglobulin-like receptors expressed on NK cells that bind MHC class I molecules and transmit inhibitory signals. KIR2DL1 is specific for HLA-C alleles with Asn77 and Lys80.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KIR2DL1/KIR2DS5 Antibody (FAB1844A)(10)

Publications using FAB1844A

• Geanes, ES;Greening, G;Aggelakopoulou, M;Truong, LH;Khanal, S;LeMaster, C;Herman, M;McLennan, R;Borrow, P;Bradley, T; Bromodomain and extra-terminal protein inhibitors modulate natural killer cell function and differentiation Scientific reports 2026-01-03 [PMID: 41484412] (Immunocytochemistry, Human)
• Mai Anh Thi Nguyen, Kohei Hosokawa, Takeshi Yoroidaka, Hiroyuki Maruyama, J. Luis Espinoza, Mahmoud I. Elbadry et al. Resistance of KIR Ligand–Missing Leukocytes to NK Cells In Vivo in Patients with Acquired Aplastic Anemia ImmunoHorizons 7/1/2020 [PMID: 32680908]
• Todd Bradley, Dimitra Peppa, Isabela Pedroza-Pacheco, Dapeng Li, Derek W. Cain, Ricardo Henao et al. RAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses Cell 10/4/2018 [PMID: 30270043]
• NK Cell Responses to Human Tick-Borne Encephalitis Virus Infection J Immunol, 2016-08-19;0(0):. 2016-08-19 [PMID: 27543616] (Flow Cytometry, Human)
• Marquardt N, Beziat V, Nystrom S, Hengst J, Ivarsson M, Kekalainen E, Johansson H, Mjosberg J, Westgren M, Lankisch T, Wedemeyer H, Ellis E, Ljunggren H, Michaelsson J, Bjorkstrom N Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells. J Immunol, 2015-02-11;194(6):2467-71. 2015-02-11 [PMID: 25672754] (Flow Cytometry, Human)
• Killig M, Friedrichs B, Meisig J, Gentilini C, Bluthgen N, Loddenkemper C, Labopin M, Basara N, Pfrepper C, Niederwieser D, Uharek L, Romagnani C Tracking in vivo dynamics of NK cells transferred in patients undergoing stem cell transplantation. Eur J Immunol, 2014-06-30;44(9):2822-34. 2014-06-30 [PMID: 24895051] (Flow Cytometry, Human)
• Charoudeh H, Terszowski G, Czaja K, Gonzalez A, Schmitter K, Stern M Modulation of the natural killer cell KIR repertoire by cytomegalovirus infection. Eur J Immunol, 2012-12-12;43(2):480-7. 2012-12-12 [PMID: 23161492] (Flow Cytometry, Human)
• Beziat V, Dalgard O, Asselah T, Halfon P, Bedossa P, Boudifa A, Hervier B, Theodorou I, Martinot M, Debre P, Bjorkstrom NK, Malmberg KJ, Marcellin P, Vieillard V CMV drives clonal expansion of NKG2C+ NK cells expressing self-specific KIRs in chronic hepatitis patients. Eur. J. Immunol., 2011-12-16;42(2):447-57. 2011-12-16 [PMID: 22105371] (Flow Cytometry, Human)
• Beziat V, Descours B, Parizot C, Debre P, Vieillard V NK cell terminal differentiation: correlated stepwise decrease of NKG2A and acquisition of KIRs. PLoS ONE, 2010-08-06;5(8):e11966. 2010-08-06 [PMID: 20700504] (Flow Cytometry, Human)
• Alici E, Sutlu T, Bjorkstrand B, Gilljam M, Stellan B, Nahi H, Quezada HC, Gahrton G, Ljunggren HG, Dilber MS Autologous antitumor activity by NK cells expanded from myeloma patients using GMP-compliant components. Blood, 2008-01-11;111(6):3155-62. 2008-01-11 [PMID: 18192509] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: KIR2DL1
• Uniprot:
  • P43626()