KIFC1 Antibody - Azide and BSA Free

Western Blot: KIFC1 Antibody [NBP3-04585] - Analysis of extracts of various cell lines, using KIFC1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Immunocytochemistry/Immunofluorescence: KIFC1 Antibody [NBP3-04585] - Analysis of U-2 OS cells using KIFC1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP3-04585] - Paraffin-embedded mouse testis using KIFC1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP3-04585] - Paraffin-embedded rat testis using KIFC1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP3-04585] - Paraffin-embedded human colon carcinoma using KIFC1 antibody at dilution of 1:100 (40x lens).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

KIFC1 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit KIFC1 Antibody - Azide and BSA Free (NBP3-04585) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human KIFC1 (NP_002254.2). MDPQRSPLLEVKGNIELKRPLIKAPSQLPLSGSRLKRRPDQMEDGLEPEKKRTRGLGATTKITTSHPRVPSLTTVPQTQGQTTAQKVSKKTGPRCSTAIATGLKNQKPVPAVPVQKSGTSGVPPMAGGKKPSKRPAWDLKGQLCDLNAELKRCRERTQTLDQENQQLQDQLRDAQQQVKALGTERTTLEGHLAKVQAQAEQGQQELKNLRACVLELEERLSTQEGLVQELQKKQVELQEERRGLMSQLEE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KIFC1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Theoretical MW: 73 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for KIFC1 Antibody - Azide and BSA Free

• HSETMGC1202
• kinesin family member C1
• kinesin-like 2
• Kinesin-like protein 2
• kinesin-like protein KIFC1
• Kinesin-related protein HSET
• KNSL2MGC149736
• MGC149737

Background

Minus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: KIFC1