| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47407-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-04585-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06407-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49370-25ul | |||
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NB100-40844] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- KIFC1/MKLP2 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-NB100-40844-img0005.jpg "Immunohistochemistry-Paraffin: KIFC1 Antibody [NB100-40844] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- KIFC1/MKLP2 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB")
![Immunoprecipitation: KIFC1 Antibody [NB100-40844] - Detection of Human KIFC1/MKLP2 on HeLa whole cell lysate using NB100-40844. KIFC1/MKLP2 was also immunoprecipitated by other rabbit anti-KIFC1/MKLP2 antibodies and NB100-40843.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunoprecipitation-NB100-40844-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: KIFC1 Antibody (2B9) [H00003833-M01] - Analysis of monoclonal antibody to KIFC1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/KIFC1-Antibody-2B9-Immunocytochemistry-Immunofluorescence-H00003833-M01-img0005.jpg "Immunocytochemistry/Immunofluorescence: KIFC1 Antibody (2B9) [H00003833-M01] - Analysis of monoclonal antibody to KIFC1 on HeLa cell. Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: KIFC1 Antibody (2B9) [H00003833-M01] - Analysis of monoclonal antibody to KIFC1 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/KIFC1-Antibody-2B9-Immunohistochemistry-Paraffin-H00003833-M01-img0007.jpg)
![ELISA: KIFC1 Antibody (2B9) [H00003833-M01] - Detection limit for recombinant GST tagged KIFC1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/KIFC1-Antibody-2B9-ELISA-H00003833-M01-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: KIFC1 Antibody [NBP2-47407] - Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47407-img0003.jpg "Immunocytochemistry/Immunofluorescence: KIFC1 Antibody [NBP2-47407] - Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome.")
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP2-47407] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP2-47407-img0007.jpg)
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP2-47407] - Staining of human tonsil shows moderate nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP2-47407-img0004.jpg)
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP2-47407] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP2-47407-img0005.jpg)
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP2-47407] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP2-47407-img0006.jpg)
![Western Blot: KIFC1 Antibody (10M10J4) [NBP3-15248] - Western blot analysis of extracts of various cell lines, using KIFC1 antibody (NBP3-15248) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/KIFC1-Antibody-10M10J4-Western-Blot-NBP3-15248-img0004.jpg "Western Blot: KIFC1 Antibody (10M10J4) [NBP3-15248] - Western blot analysis of extracts of various cell lines, using KIFC1 antibody (NBP3-15248) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: KIFC1 Antibody (10M10J4) [NBP3-15248] - Immunofluorescence analysis of U-2 OS cells using KIFC1 Rabbit mAb (NBP3-15248) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/KIFC1-Antibody-10M10J4-Immunocytochemistry-Immunofluorescence-NBP3-15248-img0003.jpg)
![Immunohistochemistry-Paraffin: KIFC1 Antibody (10M10J4) [NBP3-15248] - Immunohistochemistry of paraffin-embedded human appendix using KIFC1 Rabbit mAb (NBP3-15248) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/KIFC1-Antibody-10M10J4-Immunohistochemistry-Paraffin-NBP3-15248-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: KIFC1 Antibody (10M10J4) [NBP3-15248] - Immunofluorescence analysis of NIH-3T3 cells using KIFC1 Rabbit mAb (NBP3-15248) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/KIFC1-Antibody-10M10J4-Immunocytochemistry-Immunofluorescence-NBP3-15248-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: KIFC1 Antibody (10M10J4) [KIFC1] - Immunofluorescence analysis of NIH-3T3 cells using KIFC1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15248_rabbit-kifc1-mab-10m10j4-5220251726070.jpg)
![Immunocytochemistry/ Immunofluorescence: KIFC1 Antibody (10M10J4) [KIFC1] - Immunofluorescence analysis of U-2 OS cells using KIFC1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15248_rabbit-kifc1-mab-10m10j4-52202517251987.jpg)
![Western Blot: KIFC1 Antibody [NBP3-04585] - Analysis of extracts of various cell lines, using KIFC1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Western-Blot-NBP3-04585-img0005.jpg "Western Blot: KIFC1 Antibody [NBP3-04585] - Analysis of extracts of various cell lines, using KIFC1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![Immunocytochemistry/Immunofluorescence: KIFC1 Antibody [NBP3-04585] - Analysis of U-2 OS cells using KIFC1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04585-img0001.jpg)
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP3-04585] - Paraffin-embedded mouse testis using KIFC1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP3-04585-img0004.jpg)
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP3-04585] - Paraffin-embedded rat testis using KIFC1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP3-04585-img0002.jpg)
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP3-04585] - Paraffin-embedded human colon carcinoma using KIFC1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP3-04585-img0003.jpg)
![Western Blot: KIFC1 Antibody (JE00-46) [NBP3-32504] - Western blot analysis of KIFC1 on different lysates with Rabbit anti-KIFC1 antibody (NBP3-32504) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HT-29 cell lysate Lane 3: HeLa cell lysate Lane 4: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74 kDa Observed band size: 70 kDa Exposure time: 43 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32504) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32504_rabbit-kifc1-mab-je00-46-278202415242619.jpg "Western Blot: KIFC1 Antibody (JE00-46) [NBP3-32504] - Western blot analysis of KIFC1 on different lysates with Rabbit anti-KIFC1 antibody (NBP3-32504) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HT-29 cell lysate Lane 3: HeLa cell lysate Lane 4: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74 kDa Observed band size: 70 kDa Exposure time: 43 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32504) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: KIFC1 Antibody (JE00-46) [NBP3-32504] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-KIFC1 antibody (NBP3-32504) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32504) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32504_rabbit-kifc1-mab-je00-46-298202414262691.jpg)
![Immunocytochemistry/ Immunofluorescence: KIFC1 Antibody (JE00-46) [NBP3-32504] - Immunocytochemistry analysis of Jurkat cells labeling KIFC1 with Rabbit anti-KIFC1 antibody (NBP3-32504) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KIFC1 antibody (NBP3-32504) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32504_rabbit-kifc1-mab-je00-46-29202410334073.jpg)
![Flow Cytometry: KIFC1 Antibody (JE00-46) [NBP3-32504] - Flow cytometric analysis of Jurkat cells labeling KIFC1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32504, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32504_rabbit-kifc1-mab-je00-46-29202416453263.jpg)
![Western Blot: KIFC1 Antibody (S03-3E4) [NBP3-15052] - Western blot detection of KIFC1 in K562, 3T3, Hela cell lysates using KIFC1 Rabbit mAb (1:1000 diluted). Predicted band size: 74kDa. Observed band size: 74kDa.](http://images.novusbio.com/fullsize/KIFC1-Antibody-S03-3E4-Western-Blot-NBP3-15052-img0001.jpg "Western Blot: KIFC1 Antibody (S03-3E4) [NBP3-15052] - Western blot detection of KIFC1 in K562, 3T3, Hela cell lysates using KIFC1 Rabbit mAb (1:1000 diluted). Predicted band size: 74kDa. Observed band size: 74kDa.")
![Immunohistochemistry-Paraffin: KIFC1 Antibody (S03-3E4) [NBP3-15052] - Immunohistochemistry of KIFC1 in paraffin-embedded Human tonsil using KIFC1 Rabbit mAb at dilution 1/2](http://images.novusbio.com/fullsize/KIFC1-Antibody-S03-3E4-Immunohistochemistry-Paraffin-NBP3-15052-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: KIFC1 Antibody (S03-3E4) [NBP3-15052] - Immunofluorescence of KIFC1 (green) in Hela cells using NBP3-15052 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-15052_rabbit-kifc1-mab-s03-3e4-113202515145429.jpg)
![Western Blot: KIFC1 Antibody [NBP3-06407] - KIFC1 Antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size:74 kDa.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Western-Blot-NBP3-06407-img0001.jpg "Western Blot: KIFC1 Antibody [NBP3-06407] - KIFC1 Antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size:74 kDa.")
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP3-06407] - Immunochemical staining of human KIFC1 in human tonsil with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP3-06407-img0003.jpg)
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP3-06407] - Immunochemical staining of human KIFC1 in human lymph node with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP3-06407-img0002.jpg)
![Immunohistochemistry: KIFC1 Antibody [NBP2-49370] - Staining of human tonsil shows nuclear positivity.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-NBP2-49370-img0001.jpg "Immunohistochemistry: KIFC1 Antibody [NBP2-49370] - Staining of human tonsil shows nuclear positivity.")
![Western Blot: KIFC1 Overexpression Lysate (Adult Normal) [NBP2-08132] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KIFC1.](http://images.novusbio.com/fullsize/KIFC1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08132-img0001.jpg "Western Blot: KIFC1 Overexpression Lysate (Adult Normal) [NBP2-08132] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KIFC1.")