Eg5 Antibody

Images

 
Western Blot: Eg5 Antibody [NB500-181] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY KIF11 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE ...read more
Immunocytochemistry/ Immunofluorescence: Eg5 Antibody [NB500-181] - Staining of HeLa cells fixed in 3.5% paraformaldehyde. Cytoplasmic staining during interphase and spindle staining during mitosis.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Dr, PlSpecies Glossary
Applications WB, IB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Eg5 Antibody Summary

Immunogen
A recombinant segment of the coiled-coil domain of human Eg5 [Uniprot: P52732]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KIF11
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunoprecipitation 1:10-1:500
Application Notes
A band at ~125 kDa can be detected by Western blot. Use in immunoblotting reported in scientific literature (PMID: 28392145).
Theoretical MW
125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Eg5 Overexpression Lysate
Reviewed Applications
Read 2 Reviews rated 2.5
using
NB500-181 in the following applications:

Publications
Read Publications using
NB500-181 in the following applications:

  • 8 publications
  • WB
    7 publications

Reactivity Notes

Predicted to react with most mammalian species. Drosophila reactivity reported in scientific literature (PMID: 23888285). Mouse reactivity reported in scientific literature (PMID: 27117404). Plant reactivity reported in scientific literature (PMID: 19527496).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Purity
Unpurified

Alternate Names for Eg5 Antibody

  • Eg5
  • HKSP
  • kinesin family member 11
  • kinesin-like 1
  • Kinesin-like protein 1
  • kinesin-like protein KIF11
  • Kinesin-like spindle protein HKSP
  • Kinesin-related motor protein Eg5
  • KNSL1TRIP-5
  • thyroid receptor interacting protein 5
  • Thyroid receptor-interacting protein 5
  • TR-interacting protein 5
  • TRIP5EG5

Background

Eg5 is a mitotic motor protein required for bipolar spindle formation. The mitotic spindle is crucial for chromosome segregation during cell division.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Eg5 Antibody (NB500-181)(15)

We have publications tested in 5 confirmed species: Human, Mouse, Drosophila, Plant, Porcine.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(8)
WB
(7)
All Applications
Filter By Species
Human
(6)
Mouse
(2)
Drosophila
(1)
Plant
(1)
Porcine
(3)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB500-181 Applications Species
Letort G, Bennabi I, Dmitrieff S et al. A computational model of the early stages of acentriolar meiotic spindle assembly. Mol. Biol. Cell Jan 16 2019 [PMID: 30650011] (ICC/IF, Mouse) ICC/IF Mouse
Wan X, Zhang Y, Lan M et al. Meiotic arrest and spindle defects are associated with altered KIF11 expression in porcine oocytes Environ. Mol. Mutagen. Aug 28 2018 [PMID: 30151839] (WB, Porcine) WB Porcine
Mann BJ, Wadsworth P. Distribution of Eg5 and TPX2 in Mitosis: Insight from CRISPR tagged Cells Cytoskeleton (Hoboken) Aug 19 2018 [PMID: 30123975] (WB, ICC/IF, Human) WB, ICC/IF Human
Bickel KG, Mann BJ, Waitzman JS et al. Src family kinase phosphorylation of the motor domain of the human kinesin-5, Eg5. Cytoskeleton (Hoboken). 2017 Jun 23 [PMID: 28646493] (WB, Human) WB Human
Nalawansha DA, Gomes ID, Wambua MK, Pflum MKH. HDAC Inhibitor-Induced Mitotic Arrest Is Mediated by Eg5/KIF11 Acetylation. Cell Chem Biol. Apr 20 2017 [PMID: 28392145]
Liu Y, Wang Y, Du Z et al. Fbxo30 Regulates Mammopoiesis by Targeting the Bipolar Mitotic Kinesin Eg5. Cell Rep. 2016 Apr 20 [PMID: 27117404] (ICC/IF, Mouse) ICC/IF Mouse
Drechsler H, McAinsh AD. Kinesin-12 motors cooperate to suppress microtubule catastrophes and drive the formation of parallel microtubule bundles. Proc. Natl. Acad. Sci. U.S.A. 2016 Mar 22 [PMID: 26969727] (WB, Human) WB Human
Wilson Patricia G, Payne Tiffany. Genetic reprogramming of human amniotic cells with episomal vectors: neural rosettes as sentinels in candidate selection for validation assays. PeerJ. 2014 Nov 18 [PMID: 25426336] (ICC/IF, Human) ICC/IF Human
Salemi JD, McGilvray PT, Maresca TJ et al. Development of a Drosophila Cell-Based Error Correction Assay. Front Oncol 2013 Jul 23 [PMID: 23888285] (WB, Drosophila) WB Drosophila
Kaseda K, McAinsh AD, Cross RA. Dual pathway spindle assembly increases both the speed and the fidelity of mitosis. Biol Open 2012 Jan 15 [PMID: 23213363] (ICC/IF, Human) ICC/IF Human
Show All 15 Publications.

Reviews for Eg5 Antibody (NB500-181) (2) 2.52

Average Rating: 2.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB500-181:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot Eg5 NB500-181
Enlarge
reviewed by:
Emily Miller
WB Human 04/28/2014
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Summary

ApplicationWestern Blot
Sample TestedHeLa, Mouse brain
SpeciesHuman
LotC-2
CommentsThis antibody performs well most of the time. Occasionally there will be one non specific band, but this is rare.

Blocking

Blocking Details5% milk in TBST-30 min room temp

Primary Anitbody

Dilution Ratio1:100 2h room temp

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, HRP
Secondary Manufacturer Cat#Amersham
Secondary Concentration1:10000

Details

Detection NotesECL

Comments

CommentsThis antibody performs well most of the time. Occasionally there will be one non specific band, but this is rare.
Western Blot Eg5 NB500-181
Enlarge
reviewed by:
Kristin Werner
WB Human 12/16/2013
View

Summary

ApplicationWestern Blot
Sample TestedMouse tissues, MiaPaCa2, BxPC3, T3M4
SpeciesHuman

Blocking

Blocking Details1h 5%Milkpowder in TBS-T0,1%, room temperature

Primary Anitbody

Dilution Ratio1h 1:500-1:1000 in 5%Milkpowder in TBS-T0,1%, over night, 4°C

Secondary Antibody

Secondary DescriptionGoat Anti-rabbit IgG, HRP
Secondary Manufacturer Cat#7074 CellSignaling
Secondary Concentration1:1000 in 5%Milkpowder in TBS-T0,1%, 1h, room temperature

Details

Detection NotesImmobion Western Blot Detection Solution, 30sec-10min, knockdown experiments as controls

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Eg5 Antibody (NB500-181) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for Eg5 Antibody (NB500-181)

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Pathways for Eg5 Antibody (NB500-181)

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Recent Reviews

2.5
5
0
4
1
3
0
2
0
1
1

Emily Miller
04/28/2014
Application: WB
Species: Human

Kristin Werner
12/16/2013
Application: WB
Species: Human

Bioinformatics

Gene Symbol KIF11
Entrez
Uniprot