Eg5 Antibody

Images

 
Western Blot: Eg5 Antibody [NB500-181] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY KIF11 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE ...read more
Immunocytochemistry/ Immunofluorescence: Eg5 Antibody [NB500-181] - Staining of HeLa cells fixed in 3.5% paraformaldehyde. Cytoplasmic staining during interphase and spindle staining during mitosis.
Western Blot: Eg5 Antibody [NB500-181] - Human Eg5 rescues depletion of the Drosophila kinesin-5 family member Klp61F. Western blot analysis of cell lysates prepared from induced and uninduced; control and Klp61F ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Dr, Ma, PlSpecies Glossary
Applications WB, IB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Eg5 Antibody Summary

Immunogen
A recombinant segment of the coiled-coil domain of human Eg5 [Uniprot: P52732]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KIF11
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:1000
Application Notes
A band at ~125 kDa can be detected by Western blot. Use in immunoblotting reported in scientific literature (PMID: 28392145).
Theoretical MW
125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Eg5 Overexpression Lysate
Reviewed Applications
Read 2 Reviews rated 2.5
using
NB500-181 in the following applications:

Publications
Read Publications using
NB500-181 in the following applications:

  • 8 publications
  • IP
    1 publication
  • WB
    7 publications

Reactivity Notes

Predicted to react with most mammalian species. Drosophila reactivity reported in scientific literature (PMID: 23888285). Mouse reactivity reported in scientific literature (PMID: 27117404). Plant reactivity reported in scientific literature (PMID: 19527496).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Purity
Unpurified

Alternate Names for Eg5 Antibody

  • Eg5
  • HKSP
  • KIF11
  • kinesin family member 11
  • kinesin-like 1
  • Kinesin-like protein 1
  • kinesin-like protein KIF11
  • Kinesin-like spindle protein HKSP
  • Kinesin-related motor protein Eg5
  • KNSL1
  • KNSL1TRIP-5
  • thyroid receptor interacting protein 5
  • Thyroid receptor-interacting protein 5
  • TR-interacting protein 5
  • TRIP5
  • TRIP-5
  • TRIP5EG5

Background

Eg5 is a mitotic motor protein required for bipolar spindle formation. The mitotic spindle is crucial for chromosome segregation during cell division.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Eg5 Antibody (NB500-181)(18)

We have publications tested in 5 confirmed species: Human, Mouse, Drosophila, Plant, Porcine.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(8)
IP
(1)
WB
(7)
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Filter By Species
Human
(7)
Mouse
(2)
Drosophila
(1)
Plant
(1)
Porcine
(3)
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Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NB500-181 Applications Species
Uniyal A, Shantanu PA, Vaidya S et al. Tozasertib Attenuates Neuropathic Pain by Interfering with Aurora Kinase and KIF11 Mediated Nociception ACS chemical neuroscience Jun 2 2021 [PMID: 34027667]
Zalenski AA, Majumder S, De K, Venere M An interphase pool of KIF11 localizes at the basal bodies of primary cilia and a reduction in KIF11 expression alters cilia dynamics Sci Rep Aug 18 2020 [PMID: 32811879] (IP, Human) IP Human
Vargas-Hurtado D, Brault JB, Piolot T et al. Differences in Mitotic Spindle Architecture in Mammalian Neural Stem Cells Influence Mitotic Accuracy during Brain Development Curr. Biol. Sep 23 2019 [PMID: 31495584]
Letort G, Bennabi I, Dmitrieff S et al. A computational model of the early stages of acentriolar meiotic spindle assembly. Mol. Biol. Cell Jan 16 2019 [PMID: 30650011] (ICC/IF, Mouse) ICC/IF Mouse
Wan X, Zhang Y, Lan M et al. Meiotic arrest and spindle defects are associated with altered KIF11 expression in porcine oocytes Environ. Mol. Mutagen. Aug 28 2018 [PMID: 30151839] (WB, Porcine) WB Porcine
Mann BJ, Wadsworth P. Distribution of Eg5 and TPX2 in Mitosis: Insight from CRISPR tagged Cells Cytoskeleton (Hoboken) Aug 19 2018 [PMID: 30123975] (WB, ICC/IF, Human) WB, ICC/IF Human
Bickel KG, Mann BJ, Waitzman JS et al. Src family kinase phosphorylation of the motor domain of the human kinesin-5, Eg5. Cytoskeleton (Hoboken). 2017 Jun 23 [PMID: 28646493] (WB, Human) WB Human
Nalawansha DA, Gomes ID, Wambua MK, Pflum MKH. HDAC Inhibitor-Induced Mitotic Arrest Is Mediated by Eg5/KIF11 Acetylation. Cell Chem Biol. Apr 20 2017 [PMID: 28392145]
Liu Y, Wang Y, Du Z et al. Fbxo30 Regulates Mammopoiesis by Targeting the Bipolar Mitotic Kinesin Eg5. Cell Rep. 2016 Apr 20 [PMID: 27117404] (ICC/IF, Mouse) ICC/IF Mouse
Drechsler H, McAinsh AD. Kinesin-12 motors cooperate to suppress microtubule catastrophes and drive the formation of parallel microtubule bundles. Proc. Natl. Acad. Sci. U.S.A. 2016 Mar 22 [PMID: 26969727] (WB, Human) WB Human
Show All 18 Publications.

Reviews for Eg5 Antibody (NB500-181) (2) 2.52

Average Rating: 2.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB500-181:
Filter by Applications
Western Blot
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot Eg5 NB500-181
Enlarge
4
reviewed by:
Verified Customer
Western Blot Human 04/28/2014
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Summary

ApplicationWestern Blot
Sample TestedHeLa, Mouse brain
SpeciesHuman
LotC-2
Western Blot Eg5 NB500-181
Enlarge
1
reviewed by:
Verified Customer
Western Blot Human 12/16/2013
View

Summary

ApplicationWestern Blot
Sample TestedMouse tissues, MiaPaCa2, BxPC3, T3M4
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Eg5 Antibody (NB500-181) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Eg5 Antibody (NB500-181)

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Recent Reviews

2.5
5
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4
1
3
0
2
0
1
1

Verified Customer
04/28/2014
Application: Western Blot
Species: Human

Verified Customer
12/16/2013
Application: Western Blot
Species: Human

Bioinformatics

Gene Symbol KIF11
Entrez
Uniprot