NuMA Antibody

Images

 
Western Blot: NuMA Antibody [NB500-174] - Analysis of NuMA expression in Cos7 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: NuMA Antibody [NB500-174] - Human HeLa cells fixed with 3.5% formaldehyde and stained with NB500-174 at 1:1000. The staining pattern shows the typical pattern for NuMA in the ...read more
Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human placenta using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. Strong nuclear with moderate cytoplasmic ...read more
Immunocytochemistry/ Immunofluorescence: NuMA Antibody [NB500-174] - NuMA antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunocytochemistry/ Immunofluorescence: NuMA Antibody [NB500-174] - NuMA antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunocytochemistry/ Immunofluorescence: NuMA Antibody [NB500-174] - Dynactin and NuMA display specific, steady-state binding at mitotic minus-ends. See also Figure 1-figure supplement 1 and Videos 1-3. Representative ...read more
Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human normal breast using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. The breast ductal/acinar epithelial cells, ...read more
Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human normal spleen using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. Most of the cells developed a strong ...read more
Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human normal skin using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. Almost all the cells in the epidermal layer ...read more
Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human normal brain using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. The cells in the brain tissue depicted ...read more
Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human normal kidney using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. The cells of various tubules/ducts and ...read more
Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human normal prostate using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. The prostatic alveolar glandular ...read more
Simple Western: NuMA Antibody [NB500-174] - Image shows a specific band for NuMA in 0.2 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ma, PmSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NuMA Antibody Summary

Immunogen
Full-length recombinant human NuMA expressed in insect Sf9 cells using baculovirus expression system, then purified to near homogeneity prior to immunization.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NUMA1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:500
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:10-1:500
Application Notes
In Western blot a band can be seen at 220 kDa. IHC-Fr was reported in scientific literature.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
HeLa Whole Cell Lysate
Publications
Read Publications using
NB500-174 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for NuMA Antibody

  • centrophilin stabilizes mitotic spindle in mitotic cells
  • NMP-22
  • nuclear mitotic apparatus protein 1
  • NuMA protein
  • NuMA
  • NUMA1
  • SP-H Antigen
  • structural nuclear protein

Background

NuMA (nuclear mitotic apparatus) is a long coil-coiled protein that plays a role in the interphase and mitosis phases of a cell cycle. In interphase, it has been hypothesized to play a structural role in the nucleoskeleton that may be important in nuclear organization and functions. During mitosis, it is associated with spindle microtubule organization and chromosome positioning. Therefore, this antibody is useful as a cell cycle marker.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NuMA Antibody (NB500-174)(19)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Mammal.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(12)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
WB
(4)
All Applications
Filter By Species
Human
(9)
Mouse
(5)
Rat
(1)
Mammal
(1)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NB500-174 Applications Species
Gemble S, Simon A, Pennetier C et al. Centromere Dysfunction Compromises Mitotic Spindle Pole Integrity Curr. Biol. Sep 23 2019 [PMID: 31495582] (Human) Human
Hueschen CL, Galstyan V, Amouzgar M et al. Microtubule End-Clustering Maintains a Steady-State Spindle Shape Curr. Biol. Feb 18 2019 [PMID: 30744975] (ICC/IF, Human) ICC/IF Human
Hueschen CL, Kenny SJ, Xu K, Dumont S. NuMA recruits dynein activity to microtubule minus-ends at mitosis Elife. 2017 Nov 29 [PMID: 29185983] (ICC/IF, WB) ICC/IF, WB
di Pietro F, Valon L, Li Y et al. An RNAi Screen in a Novel Model of Oriented Divisions Identifies the Actin-Capping Protein Z beta as an Essential Regulator of Spindle Orientation. Curr. Biol. 2017 Aug 21 [PMID: 28803871] (ICC/IF, Human) ICC/IF Human
Elting MW, Prakash M, UdY DB, Dumont S. Mapping Load-Bearing in the Mammalian Spindle Reveals Local kinetochore Fiber Anchorage that Provides Mechanical Isolation and Redundancy. Curr. Biol. Jul 24 2017 [PMID: 28690110] (ICC/IF, Mammal) ICC/IF Mammal
Serra-Marques A, Houtekamer R, Hintzen D, Canty JT The mitotic protein NuMA plays a spindle-independent role in nuclear formation and mechanics bioRxiv Jan 1 2020
Sosunov A, Wu X, McGovern R et al. Abnormal mitosis in reactive astrocytes Acta Neuropathol Commun Apr 15 2020 [PMID: 32293551] (ICC/IF, Rat) ICC/IF Rat
Wang Y, Stear JH, Swain A et al. Drug Targeting the Actin Cytoskeleton Potentiates the Cytotoxicity of Low Dose Vincristine by Abrogating Actin-Mediated Repair of Spindle Defects Mol. Cancer Res. Apr 8 2020 [PMID: 32269073] (Mouse) Mouse
Decarreau J, Wagenbach M, Lynch E et al. The tetrameric kinesin Kif25 suppresses pre-mitotic centrosome separation to establish proper spindle orientation. Nat. Cell Biol. Apr 1 2017 [PMID: 28263957]
Mahale S, Kumar M, Sharma A et al. The Light Intermediate Chain 2 Subpopulation of Dynein Regulates Mitotic Spindle Orientation. Sci Rep. Dec 1 2016 [PMID: 28003657]
Show All 19 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NuMA Antibody (NB500-174). (Showing 1 - 1 of 1 FAQs).

  1. Is this antibody raised against full-length recombinant NuMA expressed in Sf9 cells?
    • With regard to the immunogen used for this antibody, I was able to verify that the product was generated against the full length protein. The antibody immunization is described in the following paper (page 695): NCBI site

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Blogs on NuMA.

NuMA: The Key to Asymmetric Cell Division
Nuclear Mitotic Apparatus protein (NuMA) is a cell cycle-related protein that acts as an organizer of the mitotic spindle during mitosis. It may be involved in coordinating the alignment of the mitotic spindle to the cellular polarity axis, which is a...  Read full blog post.

Nucleus and Mitotic Apparatus (NuMA) Protein in Cell Cycle Regulation
NuMA, the major protein of the "Nucleus and Mitotic Apparatus", is a structural protein in vertebrates involved in cell cycle regulation. It localizes to the nucleus during interphase, and accumulates at the spindle poles during mitosis and NuMA has b...  Read full blog post.

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Bioinformatics

Gene Symbol NUMA1
Entrez
Uniprot