NuMA Antibody

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Western Blot: NuMA Antibody [NB500-174] - Western blot analysis of NuMA expression in Cos7 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: NuMA Antibody [NB500-174] - NuMA antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - IHC analysis of formalin-fixed paraffin-embedded tissue section of a malignant stromal tumor of the human small bowel using rabbit polyclonal NuMA antibody ...read more
Immunocytochemistry/ Immunofluorescence: NuMA Antibody [NB500-174] - Immunofluorescent image of human HeLa cells fixed with 3.5% formaldehyde and stained with NB500-174 at 1:1000 dilution. The staining pattern shows the ...read more
Immunocytochemistry/ Immunofluorescence: NuMA Antibody [NB500-174] - NuMA antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal breast using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/ml ...read more
Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal spleen using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/ml ...read more
Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal skin using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/ml concentration. ...read more
Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal brain using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/ml ...read more
Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal kidney using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/ml ...read more
Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal prostate using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/ml ...read more
Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human placenta using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/ml concentration. ...read more
Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human bladder cancer using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/ml ...read more
Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human breast cancer using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/ml ...read more
Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using rabbit polyclonal NuMA antibody ...read more
Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human liver cancer using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/ml ...read more
Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human pulmonary squamous cell carcinoma using rabbit polyclonal NuMA antibody (NB500-174) at ...read more
Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human rectal adenocarcinoma using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/ml ...read more
Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - IHC analysis of formalin-fixed paraffin-embedded tissue section of a poorly differentiated endometrial carcinoma of human using rabbit polyclonal NuMA antibody ...read more
Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - IHC analysis of formalin-fixed paraffin-embedded tissue section of adenocarcinoma of human stomach using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/ml ...read more
Simple Western: NuMA Antibody [NB500-174] - Simple Western lane view shows a specific band for NuMA in 0.2 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ma, PmSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NuMA Antibody Summary

Immunogen
Full-length recombinant human NuMA expressed in insect Sf9 cells using baculovirus expression system, then purified to near homogeneity prior to immunization.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NUMA1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:500
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:10-1:500
Application Notes
This NuMA antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western blot, where a band can be seen at 220 kDa. Immunohistochemistry-Frozen was reported in scientific literature.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
HeLa Lysate (NB800-PC1)
Publications
Read Publications using
NB500-174 in the following applications:

Reactivity Notes

Reactive to most mammalian species tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for NuMA Antibody

  • centrophilin stabilizes mitotic spindle in mitotic cells
  • nuclear mitotic apparatus protein 1
  • NuMA protein
  • NUMA
  • SP-H antigen
  • structural nuclear protein

Background

NuMA (nuclear mitotic apparatus) is a long coil-coiled protein that plays a role in the interphase and mitosis phases of a cell cycle. In interphase, it has been hypothesized to play a structural role in the nucleoskeleton that may be important in nuclear organization and functions. During mitosis, it is associated with spindle microtubule organization and chromosome positioning. Therefore, this antibody is useful as a cell cycle marker.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NuMA Antibody (NB500-174)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(7)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
WB
(3)
All Applications
Filter By Species
Human
(6)
Mouse
(4)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB500-174 Applications Species
Decarreau J, Wagenbach M, Lynch E et al. The tetrameric kinesin Kif25 suppresses pre-mitotic centrosome separation to establish proper spindle orientation. Nat. Cell Biol. Apr 1 2017 [PMID: 28263957]
Mahale S, Kumar M, Sharma A et al. The Light Intermediate Chain 2 Subpopulation of Dynein Regulates Mitotic Spindle Orientation. Sci Rep. Dec 1 2016 [PMID: 28003657]
Sercin O, Larsimont JC, Karambelas AE et al. Transient PLK4 overexpression accelerates tumorigenesis in p53-deficient epidermis. Nat. Cell Biol. 2015 Nov 23 [PMID: 26595384] (IHC-P, Mouse) IHC-P Mouse
Kaneko Y, Suenaga Y, Islam SM et al. Functional interplay between MYCN, NCYM, and OCT4 promotes aggressiveness of human neuroblastomas. Cancer Sci. 2015 Apr 16 [PMID: 25880909] (ICC/IF, Human) ICC/IF Human
Elting MW, Hueschen CL, Udy DB, Dumont S. Force on spindle microtubule minus ends moves chromosomes. J. Cell Biol. 2014 Jul 21 [PMID: 25023517] (ICC/IF, Human)

Details:
NuMa antibody used for ICC-IF at 1:300 dilution in experiments involving PtK2 GFP-a-tubulin cells (stable line expressing human alpha-tubulin in pEGFP-C1).
ICC/IF Human
Uchida C, Hattori T, Takahashi H et al. Interaction between RB protein and NuMA is required for proper alignment of spindle microtubules. Genes Cells 2013 Dec 19 [PMID: 24350565] (ICC/IF, WB, Human) ICC/IF, WB Human
Izumi H, Kaneko Y. Evidence of asymmetric cell division and centrosome inheritance in human neuroblastoma cells Proc Natl Acad Sci 2012 Oct 11 [PMID: 23064640] (ICC/IF, Human) ICC/IF Human
Beck B, Driessens G, Goossens S et al. A vascular niche and a VEGF-Nrp1 loop regulate the initiation and stemness of skin tumours. Nature;478(7369):399-403. 2011 Oct 20. [PMID: 22012397] (IHC-Fr, Mouse) IHC-Fr Mouse
Croyle MJ, Lehman JM, O'Connor AK et al. Role of epidermal primary cilia in the homeostasis of skin and hair follicles Development 2011 May [PMID: 21429982] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Nguyen CL, Munger K. Human papillomavirus E7 protein deregulates mitosis via an association with nuclear mitotic apparatus protein 1. J Virol;83(4):1700-7. 2009 Feb. [PMID: 19052088] (WB, ICC/IF, Human, Mouse) WB, ICC/IF Human, Mouse
Show All 11 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NuMA Antibody (NB500-174). (Showing 1 - 1 of 1 FAQs).

  1. Is this antibody raised against full-length recombinant NuMA expressed in Sf9 cells?
    • With regard to the immunogen used for this antibody, I was able to verify that the product was generated against the full length protein. The antibody immunization is described in the following paper (page 695): NCBI site

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on NuMA.

NuMA: The Key to Asymmetric Cell Division
Nuclear Mitotic Apparatus protein (NuMA) is a cell cycle-related protein that acts as an organizer of the mitotic spindle during mitosis. It may be involved in coordinating the alignment of the mitotic spindle to the cellular polarity axis, which is a...  Read full blog post.

Nucleus and Mitotic Apparatus (NuMA) Protein in Cell Cycle Regulation
NuMA, the major protein of the "Nucleus and Mitotic Apparatus", is a structural protein in vertebrates involved in cell cycle regulation. It localizes to the nucleus during interphase, and accumulates at the spindle poles during mitosis and NuMA has b...  Read full blog post.

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Bioinformatics

Gene Symbol NUMA1
Entrez
Uniprot