KIFC1 Antibody (10M10J4)

Western Blot: KIFC1 Antibody (10M10J4) [NBP3-15248] - Western blot analysis of extracts of various cell lines, using KIFC1 antibody (NBP3-15248) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunocytochemistry/Immunofluorescence: KIFC1 Antibody (10M10J4) [NBP3-15248] - Immunofluorescence analysis of U-2 OS cells using KIFC1 Rabbit mAb (NBP3-15248) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: KIFC1 Antibody (10M10J4) [NBP3-15248] - Immunohistochemistry of paraffin-embedded human appendix using KIFC1 Rabbit mAb (NBP3-15248) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/Immunofluorescence: KIFC1 Antibody (10M10J4) [NBP3-15248] - Immunofluorescence analysis of NIH-3T3 cells using KIFC1 Rabbit mAb (NBP3-15248) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: KIFC1 Antibody (10M10J4) [KIFC1] - Immunofluorescence analysis of NIH-3T3 cells using KIFC1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: KIFC1 Antibody (10M10J4) [KIFC1] - Immunofluorescence analysis of U-2 OS cells using KIFC1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clone: 10M10J4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

KIFC1 Antibody (10M10J4) Summary

• Description: Novus Biologicals Rabbit KIFC1 Antibody (10M10J4) (NBP3-15248) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human KIFC1 (Q9BW19). MDPQRSPLLEVKGNIELKRPLIKAPSQLPLSGSRLKRRPDQMEDGLEPEKKRTRGLGATTKITTSHPRVPSLTTVPQTQGQTTAQKVSKKTGPRCSTAIA
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KIFC1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for KIFC1 Antibody (10M10J4)

• HSETMGC1202
• kinesin family member C1
• kinesin-like 2
• Kinesin-like protein 2
• kinesin-like protein KIFC1
• Kinesin-related protein HSET
• KNSL2MGC149736
• MGC149737

Background

Minus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KIFC1