Ki67/MKI67 Antibody - BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody [NB110-90592] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody [NB110-90592] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunohistochemistry: Ki67/MKI67 Antibody [NB110-90592] - Deregulated MYC transforms breast acini into invasive breast carcinoma in vivo. Tumours harvested in Figure 1C were stained (above) for Ki67/MKI67 and TUNEL and ...read more
Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody [NB110-90592] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were ...read more
Immunohistochemistry: Ki67/MKI67 Antibody [NB110-90592] - Detection of human lymph node. (20X)
Immunohistochemistry: Ki67/MKI67 Antibody [NB110-90592] - Detection of human lymph node. (40X)

Product Details

Summary
Reactivity Hu, Mu, ZeSpecies Glossary
Applications ChIP, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Ki67/MKI67 Antibody - BSA Free Summary

Immunogen
The immunogen for this KI67/MKI67 Antibody was made using a synthetic peptide from the internal region of Human KI67/MKI67, between amino acids 1200-1300 [Uniprot: P46013].
Localization
Nuclear
Marker
Proliferation Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MKI67
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:3200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:3200
Application Notes
Ki67/MKI67 Antibody was used for IHC-Fr (PMID: 25647012) and IHC-P (PMID: 24599134).
Theoretical MW
359 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NB110-90592 in the following applications:

Publications
Read Publications using
NB110-90592 in the following applications:

Reactivity Notes

Use in Zebrafish reported in scientific literature (PMID:32647136).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ki67/MKI67 Antibody - BSA Free

  • antigen identified by monoclonal Ki-67
  • Antigen Ki67
  • antigen KI-67
  • Ki67
  • Ki-67
  • KIA
  • Marker Of Proliferation Ki-67
  • MIB-
  • MIB-1
  • MKI67
  • PPP1R105
  • Proliferation Marker Protein Ki-67
  • proliferation-related Ki-67 antigen
  • Protein Phosphatase 1
  • Regulatory Subunit 105
  • TSG126

Background

Ki67 is a nuclear protein associated with proliferation, named after its discovery in a Hodgkin's lymphoma-derived cell line by Scholzer and Gerdes at Kiel University, Germany (1). This proliferation marker is expressed during active phases of the cell cycle (late G1, S, G2 and M), whereas the protein remains undetectable in the G0 phase. Ki67 has two main isoforms with theoretical molecular weights of 319 kDa and 359 kDa. Its abundance is tightly regulated by synthesis and degradation with an estimated half-life of 60-90 min, regardless of its position in the cell cycle. Ki67 has been shown to be involved in ribosomal biogenesis, heterochromatin maintenance, and mitotic chromosome separation (2).

Detection of Ki67 by immunostaining is commonly used as a proliferation marker in solid tumors, as well as certain hematological malignancies (3-5). The Ki67 index, which reports on positive Ki67 stained tumor cell nuclei, has been extensively studied as a prognostic biomarker in cancers such as breast cancer and cervical cancer.

References

1. Gerdes J, Schwab U, Lemke H, Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 31:13-20. PMID: 6339421

2. Starborg M, Gell K, Brundell E and Hoog C. (1996) The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J Cell Sci. 109:143-153. 1996

3. Karamitopoulou E, Perentes E, Tolnay M, Probst A. (1998) Prognostic significance of MIB-1, p53, and bcl-2 immunoreactivity in meningiomas. Hum Pathol. 29(2):140-5. PMID: 9490273

4. Geyer FC, Rodrigues DN, Weigelt B and Reis-Filho JS. (2012) Molecular classification of estrogen receptor-positive/luminal breast cancers. Adv Anat Pathol. 19(1):39-53. PMID: 22156833

5. Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, von Knebel Doeberitz M, Neumann HH, Puig-Tintore LM, Sideri M, Rehm S, Ridder R; PALMS Study Group. (2013) Screening for cervical cancer precursors with p16/Ki-67 dual-stained cytology: results of the PALMS study. J Natl Cancer Inst. 105(20):1550-7. PMID: 24096620

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ki67/MKI67 Antibody (NB110-90592)(16)

We have publications tested in 3 confirmed species: Human, Mouse, Zebrafish.

We have publications tested in 3 applications: IHC, IHC-Fr, IHC-P.


Filter By Application
IHC
(8)
IHC-Fr
(1)
IHC-P
(5)
All Applications
Filter By Species
Human
(11)
Mouse
(4)
Zebrafish
(1)
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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB110-90592 Applications Species
Ahmadipour M, Taniguchi D, Duchesneau P Et al. Use of high-rate ventilation results in enhanced recellularization of bio-engineered lung scaffolds Tissue engineering. Part C, Methods Nov 30 2021 [PMID: 34847779]
Rehman SK, Haynes J, Collignon E, et al. Colorectal Cancer Cells Enter a Diapause-like DTP State to Survive Chemotherapy Cell Jan 7 2021 [PMID: 33417860] (IHC, Human) IHC Human
Mealiea D, Boudreau E, De Silva N et al. Modeling oncolytic virus dynamics in the tumor microenvironment using zebrafish Cancer Gene Ther. Jul 10 2020 [PMID: 32647136] (IHC-P, Zebrafish) IHC-P Zebrafish
Lourenco C, Kalkat M, Houlahan KE et al. Modelling the MYC-driven normal-to-tumour switch in breast cancer Dis Model Mech Jul 26 2019 [PMID: 31350286] (IHC-P, Human) IHC-P Human
Lima-Fernandes, E;Murison, A;da Silva Medina, T;Wang, Y;Ma, A;Leung, C;Luciani, GM;Haynes, J;Pollett, A;Zeller, C;Duan, S;Kreso, A;Barsyte-Lovejoy, D;Wouters, BG;Jin, J;Carvalho, DD;Lupien, M;Arrowsmith, CH;O'Brien, CA; Targeting bivalency de-represses Indian Hedgehog and inhibits self-renewal of colorectal cancer-initiating cells Nat Commun Mar 29 2019 [PMID: 30926792] (IHC, Human) IHC Human
Kalkat M, Resetca D, Lourenco C et al. MYC Protein Interactome Profiling Reveals Functionally Distinct Regions that Cooperate to Drive Tumorigenesis. Mol. Cell. Oct 30 2018 [PMID: 30415952] (IHC, Human) IHC Human
Kato T, Jin CS, Lee D et al. Preclinical investigation of folate receptor-targeted nanoparticles for photodynamic therapy of malignant pleural mesothelioma. Int. J. Oncol. Nov 1 2018 [PMID: 30226590] (IHC, Human) IHC Human
Kato T, Lee D, Huang H et al. Personalized siRNA-nanoparticle Systemic Therapy using Metastatic Lymph Node Specimens Obtained with EBUS-TBNA in Lung Cancer. Mol. Cancer Res. 2017 Oct 09 [PMID: 28993508] (Human) Human
Ernsting MJ, Hoang B, Lohse I et al. Targeting of metastasis-promoting tumor-associated fibroblasts and modulation of pancreatic tumor-associated stroma with a carboxymethylcellulose-docetaxel nanoparticle. J Control Release. 2015 Mar 21 [PMID: 25804872] (IHC, Mouse) IHC Mouse
Tan Q, Joshua AM, Saggar JK et al. Effect of pantoprazole to enhance activity of docetaxel against human tumour xenografts by inhibiting autophagy Br. J. Cancer. 2015 Mar 03 [PMID: 25647012] (IHC-Fr, Human)

Details:
Ki-67/MKI67 antibody used for IHC-Fr on PC3 xenograft cryosections obtained from mice that were subjected or not to 24 hours of treatments with pantoprazole/PTP, docetaxel/DOC or PTP+DOC (docetaxel-treatment after pretreatment with pantoprazole). Ki67 was used as a marker of cell proliferation and the latter was found to be reduced in antimals treated with a combination of pantoprazole and docetaxel compared to the ones treated with either drugs alone (Figure 2D).
IHC-Fr Human
Show All 16 Publications.

Review for Ki67/MKI67 Antibody (NB110-90592) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB110-90592:
Filter by Applications
IF
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
IF Mouse 12/22/2010
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Summary

ApplicationImmunofluorescence
Sample Testedmouse small intestinal epithelial cell line
SpeciesMouse

Product General Protocols

View specific protocols for Ki67/MKI67 Antibody (NB110-90592): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Ki67/MKI67 Antibody (NB110-90592). (Showing 1 - 1 of 1 FAQs).

  1. Is there a clone number for this Ki67 antibody?
    • This antibody is polyclonal, and thus it does not have a clone number.

Secondary Antibodies

 

Isotype Controls

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Blogs on Ki67/MKI67.

The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

Ki67 - an established marker for labelling proliferating cells
Ki-67/MKI67 is an antigen which is expressed during G1, S, G2, and M phases of the cell cycle (mitotically active cells), but not during G0 phase (resting cells). It is a large protein with expected molecular weight of about 395 kDa, and it has a v...  Read full blog post.

Ki67 - A Crucial Cellular Proliferation Marker
The Ki67 antigen is a prototypic cell cycle-related protein expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M). It is a non-histone nuclear protein originally identified in a Hodgkin's lymphoma-derived cell line....  Read full blog post.

The Ki67 Antibody in Cell Marker Studies
The MK167, or Ki67 antibody recognizes a nuclear protein encoded by the MK167 gene. Ki167 is involved with RNA transcription and essential to cellular proliferation, being expressed by proliferating cells at all stages of the active cell cycle; it is ...  Read full blog post.

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Verified Customer
12/22/2010
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol MKI67
Entrez
Uniprot